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Endotoxin Detection ReagentsPYROSTAR™ ES-F/Plate

  • Turbidimetric Technique
  • Endotoxin Specific
  • FDA licensed
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PYROSTAR™ ES-F / Plate is a specific Turbidimetric lysate reagent specifically for 96-well microplates.
By using an absorbance microplate reader, simultaneous measurement of multiple samples is easily made possible.
The quantitative range is wide, from 0.01 to 10 EU / mL, and it can be applied to water used to manufacture pharmaceuticals, as well as to the testing of pharmaceuticals that require dilution.

Features

  • It is possible to specifically detect endotoxin without it being affected by (1 → 3) - β-D - Glucan in the sample.
  • Regarding U.S. exports, it can also be applied to the final product testing.
  • Since it can be measured with a sample volume of 50 μL per well, even a precious sample can be measured in a small amount.
  • Stable endotoxin measurement is possible, due to the minor pH impact of the measurement sample on testing.
Multi test
Catalog No. Product Name Quantitative Range
(EU/mL)
Quantity
543-10331
with CSE
PYROSTAR™ ES-F/PLATE TEST 2 mL x 4 vials 0.01-10 160 tests
540-10341
with CSE
PYROSTAR™ ES-F/PLATE TEST 5.2 mL x 4 vials 0.01-10 400 tests

CSE: 1 vial (500ng/vial)

Kit: ES-F/Plate(543-10331) reagent for 2 mL (40 tests with microplate reader) x 4 vials; Control Standard Endotoxin (CSE) x 1 vial
Kit: ES-F/Plate(540-10341) reagent for 5.2 mL (100 tests with microplate reader) x 4 vials; Control Standard Endotoxin (CSE) x 1 vial
LAL reagent solution can be stored below −15 ±5℃ for up to 2 weeks.

standard curve information

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Regression equation: log(Tg) = ー0.260424 log(C) +1.10351
Correlation coefficient: ー0.9943

Endotoxin concentration
(EU/mL)
Gelation time (min.)
0.0100 46.0
46.7
43.3
0.1000 21.2
21.5
21.2
1.000 12.0
12.1
11.9
10.000 7.4
7.4
7.5

Recovery rate of spiked endotoxins with the Turbidimetric technique

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Topics

  • LAL Basic Knowledge
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