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Endotoxin Detection ReagentsLimulus Color KY Series

  • Chromogenic Technique
  • Endotoxin Specific

Limulus Color KY series is intended for the detection of endotoxin specific by Quantitative Kinetic Chromogenic Assay(KCA).
These series offer a highly sensitive measurement.

  • Endotoxin-specific lysate, avoids false positive results from glucans
  • Available in multi-tests vials or single-test vials
  • Quantitative Kinetic-Chromogenic Assay (KCA) reagent
  • KCA can be performed in tube reader or microplate reader
  • KCA quantitative range detection limit of 0.0002 EU/mL (Single test) and 0.0005 EU/mL (Multi test).
  • Available with matched control standard endotoxin (CSE)
  • 0.1 mL or 0.2 mL sample size when used with tube reader:0.05 mL sample size when used with microplate reader
  • Single test:
    The lysate reagent for one measurement is dispensed in advance in a test tube.
  • Multi test:
    The lysate reagent which dissolve in water for BET(bacterial endotoxin test), dispense the necessary amount in reaction tubes or plates.
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Principle

The reagent is based on the color development mechanism, which is activated by the presence of endotoxin. First, a series of activations of serine protease precursors in the reagent occur, consequently triggering the final reaction, in which a clotting enzyme hydrolyzes the chromogenic substrate to release a yellow chromogen (pNA).

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Color Development Cascade Mechanism of the Limulus Color Reagent

Single test Type
Catalog No. Product Name KCA Quantitative Range
(EU/mL)
Quantity
291-53601
with CSE
Limulus Color KY Single Test Wako 0.0002 - 5 25 tests

Kit: Limulus Color reagent, 1 sample for 0.2 mL x 25 vials; Control Standard Endotoxin (CSE) x 1 vial

Multi test Type
Catalog No. Product Name KCA Quantitative Range
(EU/mL)
Quantity
291-53101
with CSE
Limulus Color KY Test Wako 0.0005 - 5 60 tests
(120 tests with microplate reader)

Kit: Limulus Color reagent for 2 mL (20 tests with tube reader / 40 tests with microplate reader) x 3 vials; Control Standard Endotoxin (CSE) x 1 vial
LAL reagent solution can be stored below −80 ℃ for up to 2 weeks.

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Tube Reader

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Microplate Reader

Topics

  • LAL Basic Knowledge
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