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Technical Report

Analytical Chemistry Technical Report

[Chromatography Q & A] What solvents are used for washing and storage after using the column?

Most of the HPLC columns currently used are commercially available packed columns packed with packing agents. In this case, the solvent filling the column at the time of release needs to be replaced w...

Analytical Chemistry Technical Report

[Chromatography Q & A] Which buffer solution is used for the HPLC mobile phase?

A mixture of water and organic solvents is often used for the mobile phase of HPLC. However, when the dissociation state of the target component changes depending on the pH, the pH should be kept cons...

Analytical Chemistry Technical Report

[Chromatography Q & A] Differences between silica gel plate and amino silica gel plate used in thin-layer chromatography (TLC)

Thin-layer chromatography (TLC) is a thin layer of silica gel, amino silica gel, alumina, polyamide resin, etc. applied on a glass plate. It is mainly used for simple and rapid confirmation of the pro...

Analytical Chemistry Technical Report

[Chromatography Q & A] What is a difference in use of acetonitrile and methanol, which are used frequently in reverse-phase HPLC analysis?

A water and organic solvents is used frequently as an eluate of reverse-phase chromatography. In particular, methanol and acetonitrile are often used as organic solvents. Methanol is cheaper than acet...

Analytical Chemistry Technical Report

[Chromatography Q & A] HPLC columns for nonionic surfactants analysis

"Solid-phase extraction-high performance liquid chromatography method" was added to the measurement method of nonionic surfactants in the Japanese water quality standard items (Notification No. 66 of ...

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