Phosphorylated Tau T181 ELISA Kit Wako
- for Immunochemistry
- Manufacturer :
- FUJIFILM Wako Pure Chemical Corporation
- Storage Condition :
- Keep at 2-10 degrees C.
- GHS :
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96Tests
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In stock in Japan |
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Kit component
96 tests
| Antibody-coated Plate | 1 plate |
|---|---|
| Phosphorylated Tau Standard | 1 tube |
| Buffer | 60 mL |
| Biotin-conjugated Antibody Solution | 100 μL |
| Peroxidase-conjugated Streptavidin Solution | 100 μL |
| Luminescent Reagent 1 | 6 mL |
| Luminescent Reagent 2 | 6 mL |
| Wash Solution(20×) | 50 mL |
| Standard Buffer | 12 mL |
| Sample Buffer | 50 mL |
| Plate Seal | 3 sheets |
Overview
Tau is a microtubule-associated protein that is mainly expressed in neurons in the central nervous system and regulates stability of microtubules. In the brain of patients with Alzheimer’s disease, the aggregates of phosphorylated Tau, and neurofibrillary tangles are formed, and the extent of formation has been reported to correlate with severity of dementia. Therefore, Tau has been studied to investigate causes of Alzheimer’s disease and to develop drugs for treating the disease. As the concentration of Tau and phosphorylated Tau in cerebrospinal fluid (CSF) of Alzheimer’s disease patients is higher than that of non-dementia patients, it is important to measure the concentration of Tau and phosphorylated Tau in CSF. This ELISA is high sensitive kit to measure Tau including phosphorylated threonine 181 (pT181) in CSF.
Features
- Small sample volume : 20 μL
- High sensitivity : 4.40 - 500 pg/mL
- Detects differences between dementia stages
Performance
| Range | 4.40 - 500 pg/mL |
|---|---|
| Specifity | Phosphorylated Tau T181 |
| Sample | Human cerebrospinal fluid(CSF)* |
| Sample volume | 20 μL |
| Assay time | Approx. 20 hours |
| Detection method | Luminescence method** |
* Not recommended for plasma and serum.
** Requires luminescence plate reader for measurement.
Assay Principle
This kit is sandwich ELISA measuring phosphorylated Tau T181 by using 2 monoclonal antibodies. One recognizes phosphorylated Tau T181, and another reacts middle region of Tau.
Measurement example
Spike-recovery test
| Human CSF1 | Not spiked | ＋20pg/mL pTau | ＋100pg/mL pTau | ＋200 pg/mL pTau | |||
|---|---|---|---|---|---|---|---|
| Measured | Measured | Recovery | Measured | Recovery | Measured | Recovery | |
| 53.7pg/mL | 73.4pg/mL | 98.5% | 147pg/mL | 93.3% | 271pg/mL | 109% | |
| Human CSF2 | Not spiked | ＋20pg/mL pTau | ＋100pg/mL pTau | ＋200 pg/mL pTau | |||
| Measured | Measured | Recovery | Measured | Recovery | Measured | Recovery | |
| 14.0pg/mL | 32.8pg/mL | 94.0％ | 107pg/mL | 93.0％ | 225pg/mL | 106% | |
Linearity of dilution test
In each test, this product showed high quantitativeness.
Human CSF measurements at different dementia stages
Cerebrospinal fluids(CSF) from patients were measured with our ELISA kit and competitor’s kit.
The samples are from Alzheimer disease (AD) , mild cognitive impairment(MCI) and non-dementia patients.
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MMSE Score Wako Company A Company B Non-AD1 30 22.8 12.1 15.4 Non-AD2 22 14.6 22.8 ND Non-AD3 29 9.7 13.2 ND Non-AD4 30 17.2 27 2.48 Non-AD5 30 24.8 33.5 0.88 MCI1 26 23.5 37.8 ND MCI2 23 67.7 85.6 0.58 MCI3 23 38.4 38.9 ND MCI4 24 ND ND ND MCI5 25 63.6 77.2 ND AD1 16 35.2 73.4 5.58 AD2 19 36.2 21.1 ND AD3 14 193 226 17.2 AD4 24 75 46.1 ND AD5 10 204 184 9.81 Red: Low reliability because of not detected or out of standard curve.
A significant difference observed between non-dementia, MCI and Alzheimer's disease.
Correlation between Tau pT181 concentration and MMSE score
MMSE score
Diagnosis Inquiry test score of cognitive function
30-28: Normal
27-24: Suspected of mild cognitive impairment
≦23: Suspected of dementia
The high correlation was observed between Tau pT181 concentration and MMSE score.
Overview / Applications
| Outline | Tau is a microtubule-associated protein that is mainly expressed in neurons in the central nervous system and regulates stability of microtubules. In the brain of patients with Alzheimer's disease, the aggregates of phosphorylated Tau, and neurofibrillary tangles are formed, and the extent of formation has been reported to correlate with severity of dementia. Therefore, Tau has been studied to investigate causes of Alzheimer's disease and to develop drugs for treating the disease. As the concentration of Tau and phosphorylated Tau in cerebrospinal fluid(CSF) of Alzheimer's disease patients is higher than that of non-dementia patients, it is important to measure the concentration of Tau and phosphorylated Tau in CSF. This ELISA is high sensitive kit to measure Tau including phosphorylated threonine 181(pT181) in CSF. Range: 4.4-500pg/mL Sample: Human CSF Sample Volume: 20uL Assay time: Approx. 20 hours Detection method: Luminescence method |
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Property
Manufacturer Information
Alias
- Tau pT181 ELISA Kit
MAPT pT181 ELISA Kit
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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