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Anti CD9, Rat Monoclonal Antibody(77B)

for Immunochemistry
Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at -20 degrees C.
  • Structural Formula
  • Label
  • Packing

If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.

SDS

Basic Information

Comparison
Product Number
Package Size
Price
Inventory
Distributor
013-28171
Barcode No
4548995082411
20uL
List Price
99.00 USD

In stock in Japan

Distributor
019-28173
Barcode No
4548995082428
100uL
List Price
330.00 USD

In stock in Japan

Document

SDS
Product Specification Sheet
Spectral Data
Certificate of Analysis
Calibration Certificate

Product Information

Cross Reactivity Human Note: This antibody doesn’t recognize the mouse and bovine CD9. It shows a little reactivity with rat CD9.
Clone No. 77B
Host Rat
Subclass IgG2b
Formulation 1×TBS aqueous solution with 50% glycerol, 0.05w/v% sodium azide, pH 7.2.
Label Unconjugated

▼Evaluating the reactivity

The reactivity of this antibody against a serum of each organisms was measured by ELISA.
When the signal value was the same level as the blank, it was judged as "non-reactive”.

Application

ELISA (Detection) 1: 2,000-1: 16,000
(Immobilization) 1: 100- 1: 1,000
Western Blot 1: 1,000 – 1: 8,000
*Please use this antibody under non-reducing condition.
Flow Cytometry 1: 200 – 1: 2,000

Please optimize the most appropriate concentration for your analysis and samples.

Application Data

▼ELISA

Immobilized this product and competitors anti CD9 antibodies, and then compared the capture performance using ELISA.

04008803_img01.png

Immobilized antibodies: Anti CD9, Rat Monoclonal Antibody(77B), Competitor’s anti CD9 antibody (each 5 μg/mL)
Exosome samples: Exosome, from COLO201 cells, purified [Code No. 052-09301]
Detection antibody: Anti CD9, Rat Monoclonal Antibody(77B), Biotin-conjugated

This product shows higher signal value and S/B ratio than competitors.

▼Western Blot

CD9 proteins from exosomes were detected by western blot.

04008803_img02.png

Samples: Exosomes from cell culture supernatant of COLO201, human serum and human heparin plasma
Primary antibody: Anti CD9, Rat Monoclonal Antibody(77B) (1 μg/mL)
Secondary antibody: Anti Rat IgG(H+L), Donkey, Peroxidase-conjugated [Code No. 564-73961] (×20,000 diution)
Detection reagent: Immunostar® Zeta [Code No. 291-72401]

CD9 protein (24kDa) in each sample (non-reducing condition) was detected with this product.

Overview / Applications

Outline This product is monoclonal antibody to CD9 that was produced from hybridoma (clone No. 77B). CD9 is a cell surface glycoprotein which belongs to the tetraspanin superfamily (TM4SF) and, is found on the surface of exosomes. This is known to form a complex with integrins and other TM4SF, can modulate cell adhesion and migration. Also, this induces platelet activation and aggregation.
This product is intended for laboratory use only.
This product is monoclonal antibody to CD9 that was produced from hybridoma (clone No. 77B). CD9 is a cell surface glycoprotein which belongs to the tetraspanin superfamily (TM4SF) and, is found on the surface of exosomes. This is known to form a complex with integrins and other TM4SF, can modulate cell adhesion and migration. Also, this induces platelet activation and aggregation.
This antibody does't show cross reactivity with bovine. And, This can be used as immobilized antibody in ELISA.
This product is intended for laboratory use only.
Application Western blotting 1: 1,000-1: 8,000
*Use this antibody under non-reducing condition.
ELISA (detection) 1: 2,000-1: 16,000 (immobilization) 1: 100-1: 1,000
Flow cytometry 1: 200-1: 2,000

Please optimize the most appropriate concentration for your analysis and samples.

Property

Specificity Human CD9
Note: It shows a little reactivity with rat CD9.
Composition 1xTBS aqueous solution with 50w/v% glycerol, 0.05w/v% sodium azide.
Concentration Indicated on the label.

Manufacturer Information

Alias

Product content may differ from the actual image due to minor specification changes etc.

877-714-1920

Hours of Operation: 8:00 - 17:00 (EST)For other hours than the above, please contact us via the inquiry form.