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Anti CD9, Monoclonal Antibody (1K)

for Immunochemistry
Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at -20 degrees C.
  • Structural Formula
  • Label
  • Packing
SDS
Comparison
Product Number
Package Size
Price
Availability
Certificate of Analysis
Purchase
Distributor
018-27761
Barcode No
4548995071040
20uL
Discontinued
Distributor
014-27763
Barcode No
4548995071057
100uL
List Price
330.00 USD

In stock in Japan

Document

SDS
Product Specification Sheet
Package Insert
Spectral Data
Certificate of Analysis
Calibration Certificate

Product Information

Cross Reactivity Human, Bovine
[Note] This antibody doesn’t recognize the mouse and rat CD9.
Clone No. 1K
Host Mouse
Subclass IgG1
Formulation 1×TBS aqueous solution with 50% glycerol, 0.05w/v% sodium azide, pH 7.4
Label Unconjugated

▼Evaluating a reactivity
The reactivity of this antibody against a serum of each organisms was measured by ELISA.
When the signal value was the same level as the blank, it was judged as "non-reactive".

Application

ELISA 1:1,000 – 1:16,000
Western Blot 1:500 - 1:2,000 *Please use this antibody under non-reducing condition
Flow cytometry 1:500 – 1:2,000
Immunoprecipitation 5-20 μg/assay

Please optimize the most appropriate concentration for your analysis and samples.

Application Data

Western Blot

CD9 proteins on exosome isolated using our kit were detected with this product and competitor antibody using a western blot method in non-reducing conditions.

03560178_img01R.png



Sample Exosomes from cell culture supernatant of COLO201
Exosome
isolation
MagCapture™ Exosome Isolation Kit PS [code No.: 293-77601]
Primary
antibody
Anti CD9, Monoclonal Antibody (1K) [code No.014-27763] and competitive antibodies. (each 1,000 ng/mL)
Secondary
antibody
Anti Mouse IgG (H+L), Peroxidase-conjugated (dilution ratio is 1: 1,000)
Detection
reagent
ImmunoStar® Zeta [code No.291-72401]
This product is higher detection sensitivity of CD9 than competitor antibody.

ELISA

Using this product and competitor antibody as primary antibody, preprocessed exosomes* from cell culture supernatant of COLO201 was measured by ELISA kit of our product.

*Preproess: 10,000×g, 30 min

03560178_img02R.png








Sample exosomes* from cell culture supernatant of COLO201
ELISA Kit PS Capture™ Exosome ELISA Kit (Anti Mouse IgG POD) [code No. 297-79201]
Primary
antibody
Anti CD9, Monoclonal Antibody (1K) [014-27763] and competitive antibody.
This product is higher detection sensitivity of CD9 than competitor antibody.

Flow cytometry

Preprocessed exosomes* from cell culture supernatant of COLO201 was isolated using our flow cytometry kit. After this product, competitor antibody and fluorescence-labeled anti mouse antibody (secondary antibody) reacted with exosomes, antigens on exosome were detected by flow cytometry.

*Preprocess: 10,000×g, 30 min

03560178_img05.png





Sample Exosomes from cell culture supernatant of COLO201
Exosome
isolation
PS Capture™ Exosome Flow Cytometry Kit [code No.: 297-79701]
Primary
antibodies
Anti CD9, Monoclonal Antibody (1K)[code No.014-27763]and competitive antibodies.
(each dilution ratio is 1:1,000)
Secondary
antibody
Fluorescence-labeled anti mouse antibody [code No.: 569-79631]
Detection
reagent
HRP-Streptavidin, ImmunoStar® Zeta [code No.291-72401]
This product can detect CD9 on enxosomes with sensitivity equal to or higher than competitor antibody.

Overview / Applications

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Manufacturer Information

Alias

For research use or further manufacturing use only. Not for use in diagnostic procedures.

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