Anti CD9, Monoclonal Antibody (1K)
- for Immunochemistry
- Manufacturer :
- FUJIFILM Wako Pure Chemical Corporation
- Storage Condition :
- Keep at -20 degrees C.
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- Structural Formula
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20uL
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Discontinued
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100uL
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In stock in Japan |
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Document
Product Information
| Cross Reactivity | Human, Bovine [Note] This antibody doesn’t recognize the mouse and rat CD9. |
|---|---|
| Clone No. | 1K |
| Host | Mouse |
| Subclass | IgG1 |
| Formulation | 1×TBS aqueous solution with 50% glycerol, 0.05w/v% sodium azide, pH 7.4 |
| Label | Unconjugated |
▼Evaluating a reactivity
The reactivity of this antibody against a serum of each organisms was measured by ELISA.
When the signal value was the same level as the blank, it was judged as "non-reactive".
Application
| ELISA | 1:1,000 – 1:16,000 |
|---|---|
| Western Blot | 1:500 - 1:2,000 *Please use this antibody under non-reducing condition |
| Flow cytometry | 1:500 – 1:2,000 |
| Immunoprecipitation | 5-20 μg/assay |
Please optimize the most appropriate concentration for your analysis and samples.
Application Data
Western Blot
CD9 proteins on exosome isolated using our kit were detected with this product and competitor antibody using a western blot method in non-reducing conditions.
| Sample | Exosomes from cell culture supernatant of COLO201 |
|---|---|
| Exosome isolation |
MagCapture™ Exosome Isolation Kit PS [code No.: 293-77601] |
| Primary antibody |
Anti CD9, Monoclonal Antibody (1K) [code No.014-27763] and competitive antibodies. (each 1,000 ng/mL) |
| Secondary antibody |
Anti Mouse IgG (H+L), Peroxidase-conjugated (dilution ratio is 1: 1,000) |
| Detection reagent |
ImmunoStar® Zeta [code No.291-72401] |
| This product is higher detection sensitivity of CD9 than competitor antibody. |
ELISA
Using this product and competitor antibody as primary antibody, preprocessed exosomes* from cell culture supernatant of COLO201 was measured by ELISA kit of our product.
*Preproess: 10,000×g, 30 min
| Sample | exosomes* from cell culture supernatant of COLO201 |
|---|---|
| ELISA Kit | PS Capture™ Exosome ELISA Kit (Anti Mouse IgG POD) [code No. 297-79201] |
| Primary antibody |
Anti CD9, Monoclonal Antibody (1K) [014-27763] and competitive antibody. |
| This product is higher detection sensitivity of CD9 than competitor antibody. |
Flow cytometry
Preprocessed exosomes* from cell culture supernatant of COLO201 was isolated using our flow cytometry kit. After this product, competitor antibody and fluorescence-labeled anti mouse antibody (secondary antibody) reacted with exosomes, antigens on exosome were detected by flow cytometry.
*Preprocess: 10,000×g, 30 min
| Sample | Exosomes from cell culture supernatant of COLO201 |
|---|---|
| Exosome isolation |
PS Capture™ Exosome Flow Cytometry Kit [code No.: 297-79701] |
| Primary antibodies |
Anti CD9, Monoclonal Antibody (1K)［code No.014-27763］and competitive antibodies. (each dilution ratio is 1:1,000) |
| Secondary antibody |
Fluorescence-labeled anti mouse antibody [code No.: 569-79631] |
| Detection reagent |
HRP-Streptavidin, ImmunoStar® Zeta [code No.291-72401] |
| This product can detect CD9 on enxosomes with sensitivity equal to or higher than competitor antibody. |
Overview / Applications
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Manufacturer Information
Alias
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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