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Anti CD63, Monoclonal Antibody (3-13)

for Immunochemistry
Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at -20 degrees C.
Source :
Human
Host :
Mouse
Clone No. :
3-13
Classification :
Monoclonal
  • Structural Formula
  • Label
  • Packing
SDS
Comparison
Product Number
Package Size
Price
Availability
Certificate of Analysis
Purchase
Distributor
012-27063
Barcode No
4548995060907
100uL
List Price
330.00 USD

Out of Stock

Document

SDS
Product Specification Sheet
Package Insert
Spectral Data
Certificate of Analysis
Calibration Certificate

Features

  • High-sensitive detection
  • Low cost
  • Specific to human CD63*1

*1 Does not recognize mouse, rat, and bovine CD63.

Product Information

Cross Reactivity Human
Note: This antibody doesn’t recognize the mouse, rat and bovine CD63.
Clone No. 3-13
Host Mouse
Subclass IgG1
Formulation 1×TBS aqueous solution with 50% glycerol, 0.05w/v% sodium azide, pH 7.2
Label Unconjugated

▼Evaluating a reactivity
The reactivity of this antibody against a serum of each organisms was measured by ELISA.
When the signal value was the same level as the blank, it was judged as "non-reactive".

Application

ELISA 1: 1,000 - 1: 16,000
Western Blot 1: 500 - 1: 2,000 *Please use this antibody under non-reducing condition
Flow cytometry 1: 100 - 1: 1,000
Immunoprecipitation 5 - 50 μg/assay

Please optimize the most appropriate concentration for your analysis and samples.

Application Data

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Exosomes were purified from COLO201 cell culture supernatant and used for comparison of detection sensitivity of Western Blotting.


This product was capable of detecting CD63 protein on exosome surface at a sensitivity higher than those of Competitors A and B.

Exosome isolation MagCapture™ Exosome Isolation Kit PS (Code No. 293-77601)
Gel SuperSep™Ace 5-20%, 17 wells (Code No. 194-15021)
Running buffer SDS-PAGE Buffer, pH8.5 (Code No. 192-16801)
Blocking 3% Skimmed milk/PBS-T
Chemiluminescent reagent ImmunoStar® Zeta (Code No. 295-72404)
Primary antibody diluted 1,000-fold
Secondary antibody anti-mouse IgG (H+L, peroxidase-conjugated, diluted 10,000- fold) (for Competitor B, the secondary antibody included in the product was used)

Overview / Applications

Outline This product is for research use only. Do not administer it to human.
It is a mouse monoclonal antibody against CD63 (lysosome-associated membrane glycoprotein 3: LAMP3) produced from hybridoma (Clone No. 3-13).
CD63 is a membrane protein with four transmembrane domains and is used as a marker protein for activated blood platelets. It is also present in monocytes and macrophages, and it is known to be weakly expressed in granulocytes, B cells and T cells.
In recent years, CD63 has been used as a marker of extracellular vesicles, and proteins, microRNAs, DNA and the like contained in these vesicles attract attention.
[Solution composition] 1 x TBS (pH 7.2), 50% glycerol, 0.05 w/v% sodium azide
[Immunized animals] mouse
[Clone No.] 3-13
[Bound antibody subclass] IgG1
[Storage conditions] -20°C

Property

Specificity Human CD63
*This antibody doesn't react with Mouse, Rat and Bovine CD63.
[Reactivity confirmation condition]
The reactivity of this antibody against the serum of each organisms was measured by ELISA.
When the signal value was the same level as the blank, it was judged as "non-reactive.

Antibody Information

Antigen CD63
Antigen Alias CD63
Melanoma Assoc. Ag
Melanoma Assoc. Antigen
GRANULOPHYSIN
OMA81H
TETRASPANIN-30
TSPAN-30
LIMP
Lysosomal Membrane Glycoprotein CD63 (CD63)
Lysosomal Membrane Glycoprotein CD63
Lysosomal-associated Membrane Protein 3 (LAMP3)
MLA1
LAMP-3
Melanoma-associated antigen
ME491
Pltgp40
gp55
Melanoma Associated Ag
Subclass IgG1
Labeling Unlabeled

Manufacturer Information

Alias

For research use or further manufacturing use only. Not for use in diagnostic procedures.

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