Anti CD63, Monoclonal Antibody (3-13)
- for Immunochemistry
- Manufacturer :
- FUJIFILM Wako Pure Chemical Corporation
- Storage Condition :
- Keep at -20 degrees C.
- Source :
- Human
- Host :
- Mouse
- Clone No. :
- 3-13
- Classification :
- Monoclonal
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- Structural Formula
- Label
- Packing
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100uL
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In stock in Japan |
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Document
Features
- High-sensitive detection
- Low cost
- Specific to human CD63*1
*1 Does not recognize mouse, rat, and bovine CD63.
Product Information
| Cross Reactivity | Human Note: This antibody doesn’t recognize the mouse, rat and bovine CD63. |
|---|---|
| Clone No. | 3-13 |
| Host | Mouse |
| Subclass | IgG1 |
| Formulation | 1×TBS aqueous solution with 50% glycerol, 0.05w/v% sodium azide, pH 7.2 |
| Label | Unconjugated |
▼Evaluating a reactivity
The reactivity of this antibody against a serum of each organisms was measured by ELISA.
When the signal value was the same level as the blank, it was judged as "non-reactive".
Application
| ELISA | 1: 1,000 - 1: 16,000 |
|---|---|
| Western Blot | 1: 500 - 1: 2,000 *Please use this antibody under non-reducing condition |
| Flow cytometry | 1: 100 - 1: 1,000 |
| Immunoprecipitation | 5 - 50 μg/assay |
Please optimize the most appropriate concentration for your analysis and samples.
Application Data
Exosomes were purified from COLO201 cell culture supernatant and used for comparison of detection sensitivity of Western Blotting.
This product was capable of detecting CD63 protein on exosome surface at a sensitivity higher than those of Competitors A and B.
| Exosome isolation | MagCapture™ Exosome Isolation Kit PS (Code No. 293-77601) |
| Gel | SuperSep™Ace 5-20%, 17 wells (Code No. 194-15021) |
| Running buffer | SDS-PAGE Buffer, pH8.5 (Code No. 192-16801) |
| Blocking | 3% Skimmed milk/PBS-T |
| Chemiluminescent reagent | ImmunoStar® Zeta (Code No. 295-72404) |
| Primary antibody | diluted 1,000-fold |
| Secondary antibody | anti-mouse IgG (H+L, peroxidase-conjugated, diluted 10,000- fold) (for Competitor B, the secondary antibody included in the product was used) |
Overview / Applications
| Outline | This product is for research use only. Do not administer it to human. It is a mouse monoclonal antibody against CD63 (lysosome-associated membrane glycoprotein 3: LAMP3) produced from hybridoma (Clone No. 3-13). CD63 is a membrane protein with four transmembrane domains and is used as a marker protein for activated blood platelets. It is also present in monocytes and macrophages, and it is known to be weakly expressed in granulocytes, B cells and T cells. In recent years, CD63 has been used as a marker of extracellular vesicles, and proteins, microRNAs, DNA and the like contained in these vesicles attract attention. [Solution composition] 1 x TBS (pH 7.2), 50% glycerol, 0.05 w/v% sodium azide [Immunized animals] mouse [Clone No.] 3-13 [Bound antibody subclass] IgG1 [Storage conditions] -20°C |
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Property
| Specificity | Human CD63 *This antibody doesn't react with Mouse, Rat and Bovine CD63. [Reactivity confirmation condition] The reactivity of this antibody against the serum of each organisms was measured by ELISA. When the signal value was the same level as the blank, it was judged as "non-reactive. |
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Antibody Information
| Antigen | CD63 |
|---|---|
| Antigen Alias | CD63 Melanoma Assoc. Ag Melanoma Assoc. Antigen GRANULOPHYSIN OMA81H TETRASPANIN-30 TSPAN-30 LIMP Lysosomal Membrane Glycoprotein CD63 (CD63) Lysosomal Membrane Glycoprotein CD63 Lysosomal-associated Membrane Protein 3 (LAMP3) MLA1 LAMP-3 Melanoma-associated antigen ME491 Pltgp40 gp55 Melanoma Associated Ag |
| Subclass | IgG1 |
| Labeling | Unlabeled |
Manufacturer Information
Alias
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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