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Anti CD63, Monoclonal Antibody (3-13), Red Fluorochrome(635) Conjugated

for Immunochemistry
Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at 2-10 degrees C.
  • Structural Formula
  • Label
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SDS
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Price
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Certificate of Analysis
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Distributor
011-27751
Barcode No
4548995070883
25Tests
List Price
143.00 USD

Out of Stock

Distributor
017-27753
Barcode No
4548995070890
100Tests
List Price
495.00 USD

Out of Stock

Document

SDS
Product Specification Sheet
Package Insert
Spectral Data
Certificate of Analysis
Calibration Certificate

Product Information

Cross Reactivity Human
[Note] This antibody doesn’t recognize the mouse, rat and bovine CD63.
Clone No. 3-13
Host Mouse
Subclass IgG1
Formulation 1×TBS aqueous solution with Tween 20, the stabilizer, and preservative
Label Red Fluorochrome(635)
Excitation: 634 nm, Emission: 654 nm

▼Evaluating a reactivity
The reactivity of this antibody against a serum of each organisms was measured by ELISA.
When the signal value was the same level as the blank, it was judged as "non-reactive".

Application

Flow Cytometric analysis of exosomes: 10uL (1 test) product for 33uL or more culture supernatant/biofluids os recommended for the exosome flow cytometry using PS Capture Exosome Flow Cytometry Kit [code No. 297-79701].

Please optimize the most appropriate concentration for your analysis and samples.

Application Data

Flow cytometry

Ex / Em = 634 nm / 654 nm

FCM analysis of COLO201 cells

Flow cytometric analysis of COLO201 cells was performed using Wako (3-13) and competitor A.

Sample amount: 1 x 106 cells
Antibody amount: 10 μL (1 test)

Detection of CD63 on the surface of exosome in COLO201 cell culture supernatant and human serum

From pretreated COLO201 cell conditioned medium (8-fold dilution) and human serum, exosomes were isolated using PS Capture™ Exosome Flow Cytometry Kit (Code No. 297-79701) and analyzed by flow cytometry using this antibody or the other company product.

※ Pretreatment condition: 10,000 x g, 30min.

Sample amount (supernatant / serum): 33 μL

This product achieved detection of CD63 on the COLO201 cell surface and exosome surface comparable to or more sensitive than the other company product.

Overview / Applications

Outline Anti human CD63 (lysosome-associated membrane glycoprotein 3 : LAMP3) mouse monoclonal antibody was produced by hybridoma, clone number; 3-13. CD63 is the four-transmembrane protein and used for the marker protein for activated platelet.
Recently, CD63 is also used for a marker protein for extracellular vesicles, which contain proteins, DNAs, mRNAs, microRNAs and are expected as a novel biomarker.
This product is antibody conjugated red fluorochrome (635) which is similar to Cy5 and FUJIFILM Wako's original compound to Anti CD63, Monoclonal Antibody (3-13).
It is suitable for Flow Cytometry experiment.
Excitation : 634 nm, Emission : 654 nm
Purpose Flow Cytometry
Instructions Flow Cytometric analysis of exosomes: 10uL (1 test) product for 33uL or more culture supernatant/biofluids os recommended for the exosome flow cytometry using PS Capture Exosome Flow Cytometry Kit [code No. 297-79701].

Note: The particle size of exosome is too small to detect by Flow Cytometry. To using Anti CD63, Monoclonal Antibody(3-13), Red Fluorochrome conjugated, please use with the exosome capture beads.
Please optimize the most appropriate concentration for your analysis and samples.

Flow cytometric analysis of cells: 10uL (1 test) product for 1 x 10^6 cells.

Property

Specificity Specific for human CD63.
*This antibody doesn't recognize the mouse, rat, and bovine CD63.
[Reactivity confirmation condition]
The reactivity of these antibodies against the serum of each organisms was measured by ELISA.
When the signal value was the same level as the blank, it was judged as "non-reactive.
Composition 1 x TBS aqueous solution with Tween 20, the stabilizer, and the preservative.

Manufacturer Information

Alias

For research use or further manufacturing use only. Not for use in diagnostic procedures.

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