Anti CD81, Rat Monoclonal Antibody (9B), Biotin-conjugated
- for Immunochemistry
- Manufacturer :
- FUJIFILM Wako Pure Chemical Corporation
- Storage Condition :
- Keep at -20 degrees C.
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50uL
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In stock in Japan |
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Product Information
| Cross Reactivity | Human [Note] This antibody doesn’t recognize the mouse and rat CD81, but slightly recognize bovine CD81. |
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| Clone No. | 9B |
| Host | Rat |
| Subclass | IgG2b |
| Formulation | 1×TBS aqueous solution with 50% glycerol, 0.05w/v% sodium azide, pH 7.2 |
| Label | Biotin |
▼Evaluating a reactivity
The reactivity of this antibody against a serum of each organisms was measured by ELISA.
When the signal value was the same level as the blank, it was judged as "non-reactive".
Application
| ELISA | 1: 2,000 – 1: 16,000 |
|---|---|
| Western Blot | 1: 1,000 - 1: 8,000 *Please use this antibody under non-reducing condition |
| Immunoprecipitation | 5-20 μg/assay |
Please optimize the most appropriate concentration for your analysis and samples.
Application Data
Detection of exosomes by ELISA
Detection sensitivity of EVs was compared using ELISA kit of our product.
| EVs Sample | Exosomes, from COLO201 cells, purified [code No. 052-09301] |
|---|---|
| ELISA Kit | PS CaptureTM Exosome ELISA Kit (Streptavidin HRP)[code No. 298-80601] |
| Primary Antibody |
Anti CD81, Rat Monoclonal Antibody (9B), Biotin-conjugated [code No.011-28111] and Competitive biotin-conjugated antibodies (each 250 ng/mL) |
| This product is lower blank value and higher EVs signal value than competitor antibodies. |
Directly detection of exosomes in biofluids by ELISA
Using this product as primary antibody, Exosomes from biofluids samples was detected by ELISA kit of our product.
| Sample | human serum and EDTA plasma |
|---|---|
| ELISA Kit | PS Capture™ Exosome ELISA Kit (Streptavidin HRP) [code No. 298-80601] |
| Primary antibody |
Anti CD81, Rat Monoclonal Antibody (9B), Biotin-conjugated [code No. 011-28111] (250 ng/mL) |
| Exosomes in biofluids were detected concentration-dependently with this product. |
Western Blot
CD81 proteins on exosomes isolated using our kit were detected with this product using western blot method in non-reducing conditions.
| Sample | Exosomes from cell culture supernatant of COLO201, human serum and human heparin plasma |
|---|---|
| Exosome isolation |
MagCapture™ Exosome Isolation Kit PS [code No.: 293-77601] |
| Detection antibody |
Anti CD81, Rat Monoclonal Antibody (9B), Biotin-conjugated [code No.011-28111] (500 ng/mL) |
| Detection reagent |
HRP-Streptavidin, ImmunoStar® Zeta [code No.291-72401] |
| CD81 protein (26kDa) in each samples was detected with this product. |
Overview / Applications
| Outline | CD81 is 26 kDa cell surface protein and is known as TAPA-1 (Target of the Antiproliferative Antibody 1) and Tetraspanin-28 (Tspan-28). CD81 interacts directly with immunoglobulin superfamily member 8 (IGSF8, CD316) and CD36. It also forms a signal transduction complex with CD19, CD21 and Leu-13 (CD225) on the surface of B cell. In addition, on T cells CD81 associates with CD4 and CD8 and provides a costimulatory signal with CD3. Anti CD81, Rat Monoclonal Antibody (9B) is established against human CD81 with Rat. This antibody is labeled with biotin. [Clone No.] 9B [Immunized animal] Rat [Antibody subclass] IgG2b [Label] Biotin |
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| Instructions | ELISA 1 : 2,000 - 1 : 16,000 Western Blot 1 : 1,000 - 1 : 8,000 Immunoprecipitation 5-20ug/assay Please optimize the most appropriate concentration for your analysis and samples. |
Property
| Specificity | React with Human CD81. *Not react with Mouse and Rat CD81. *React only slightly with Bovine CD81. [Reactivity confirmation condition] The reactivity of this antibody against the serum of each organisms was measured by ELISA. When the signal value was the same level as the blank, it was judged as "not react". |
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| Composition | 1xTBS aqueous solution with 50w/v% glycerol, 0.05w/v% sodium azide, pH7.2. |
Manufacturer Information
Alias
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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