Anti CD81, Monoclonal Antibody (17B1)
- for Immunochemistry
- Manufacturer :
- FUJIFILM Wako Pure Chemical Corporation
- Storage Condition :
- Keep at -20 degrees C.
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20uL
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Discontinued
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100uL
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In stock in Japan |
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Document
Product Information
| Cross Reactivity | Human, Bovine [Note] It dose not react with mouse and rat CD81 |
|---|---|
| Clone No. | 17B1 |
| Host | Mouse |
| Subclass | IgG2b |
| Formulation | 1×TBS aqueous solution with 50% glycerol, 0.05%w/v sodium azide, pH7.4 |
| Label | unlabeled |
▼Evaluating a reactivity
The reactivity of this antibody against a serum of each organisms was measured by ELISA.
When the signal value was the same level as the blank, it was judged as "non-reactive".
Application
| ELISA | 1 : 2,000 – 1 : 16,000 |
|---|---|
| Western Blot | 1 : 1,000 – 1 : 8,000 *Please use this antibody under non-reducing condition |
| Flow Cytometry | 1 : 250 – 1 : 1,000 |
| Immunoprecipitation | 5 ‒ 50μg/assay |
Please optimize the most appropriate concentration for your analysis and samples.
Application Data
Western Blot
Exosomes (1.25 to 20 ng) isolated and purified from COLO201 cell conditioned medium using MagCapture™ Exosome Isolation Kit PS (Code No. 293-77601) were detected with this antibody or competitor A.
Isolation of Exosomes: MagCapture™ Exosome Isolation Kit PS (Code No. 293-77601)
Gel: SuperSep™ ACE 10-20%, 17well (Code No. 198-15041)
Runnning Buffer: SDS-PAGE Buffer, pH8.5 (Code No. 192-16801)
Blocking: 3% Skim milk / PBS-T
Chemiluminescent reagent: ImmunoStar® Zeta (Code No. 295-72404)
Primary Antibody: 2,000-fold dilution
Secondary Antibody: Anti Mouse IgG (H+L), Peroxidase Conjugated, 10,000-fold dilution.
ELISA
Exosomes in pretreated※ 500-fold diluted COLO201 cell conditioned medium were measured using this antibody or competitor A as the primary antibody. As reagents other than the primary antibody, ones included in PS Capture™ Exosome ELISA Kit (Anti Mouse IgG POD) (Code No. 297-79201) were used.
※ Pretreatment condition: 10,000 x g, 30min.
Flow Cytometry
From pretreated※ 8-fold diluted COLO201 cell conditioned medium, exosomes were isolated using PS Capture™ Exosome Flow Cytometry Kit (Code No. 297-79701) and subjected to detection of the exosome surface antigen by flow cytometry using this antibody or competitor A and fluorescence-labeled anti-mouse IgG antibody (Code No. 569-79631).
※ Pretreatment condition: 10,000 x g, 30min.
Immunoprecipitation
Exosomes in pretreated※ COLO201 cell conditioned medium were captured by immunoprecipitation using this antibody or competitor A and detected using HRP-labeled anti-CD9 antibody (competitor B).
※ Pretreatment condition: 10,000 x g, 30min.
Gel: SuperSep™ ACE 10-20%, 17well (Code No. 198-15041)
Running Buffer: SDS-PAGE Buffer, pH8.5 (Code No. 192-16801)
Blocking: 3% Skim milk/PBS-T
Chemiluminescent reagent: ImmunoStar® Zeta (Code No. 295-72404)
HRP conjugated Anti-CD81 antibody: 1,000-fold dilution
COLO201 cell culture supernatant: 1 mL
Antibody amount: 5 μg
This product successfully detected CD81 on the exosome surface at a high sensitivity. Also, CD81 positive exosomes were efficiently collected.
Overview / Applications
Property
| Specificity | Human CD81, Bovine CD81 *Does not react with mouse and rat CD81. [Reactivity confirmation condition] The reactivity of this antibody against the serum of each organisms was measured by ELISA. When the signal value was the same level as the blank, it was judged as "non-reactive. |
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Manufacturer Information
Alias
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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