Column for HPLC

High performance liquid chromatography (HPLC) is mainly used for fractionation and analysis, and separation of the analyte is performed by the column.
Wakopak® series is a column for HPLC and UHPLC using a packing agent based on a fully porous high-purity silica gel. FUJIFILM Wako offers columns for various separation modes, including application-specific columns for analysis (pesticides, amino acids, aldehyde-ketones, surfactants, etc.) as well as polymer columns, optical isomer separation columns, SFC columns, and core-shell columns.

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How to Select HPLC Columns

The separation of HPLC differs depending on the combination of column and mobile phase. There are various factors for selection of mobile phase such as type of solvent, mobile phase composition ratio and pH, and the corresponding column selection is necessary. In addition, column length, inside diameter and particle size are also important factors.
The following table summarizes the information on Wakopak® series (packing agent, particle size, column pressure, pH range, etc.). Please use it when selecting columns.

Select from USP L Column Listing

The USP classifies the packing agents used for HPLC by attribute. The correspondence between the columns of FUJIFILM Wako and USP L number is summarized below. Please use it when selecting columns.

USP L## Functional group Definitions Wakopak®
L1 C18 Octadecylsilane chemically bonded to porous or non-porous silica or
ceramic microparticles, 1.5 to 10 µm in diameter.
Combi ODS
Combi ODS fast
eco-ODS
Handy ODS
Navi C18-5
Ultra C18-2
Ultra C18-3
Ultra C18-5
Wakosil-Ⅱ 3C18 AR
Wakosil-Ⅱ 3C18 HG
Wakosil-Ⅱ 3C18 RS
Wakosil 5C18
Wakosil-Ⅱ 5C18
Wakosil-Ⅱ 5C18-100
Wakosil 5C18-200
Wakosil 5C18-200N
Wakosil 5C18-200T
Wakosil 5C18AR
Wakosil-Ⅱ 5C18 AR
Wakosil-Ⅱ 5C18 AR Prep
Wakosil-Ⅱ 5C18 HG
Wakosil-Ⅱ 5C18 HG Prep
Wakosil 5C18N
Wakosil-Ⅱ 5C18 RS
Wakosil-Ⅱ 5C18 RS Prep
Wakosil 5C18T
Wakosil 7C18
Wakosil 10C18
Wakosil 10C18-200
Wakosil-Cu
Wakosil-PAHs
L3 SIL Porous silica particles, 1.5 to 10 µm in diameter. Wakosil-Ⅱ 5SIL-AQ
Wakosil 5SIL
Wakosil 5SIL-120
Wakosil-Ⅱ 5SIL Prep
Wakosil 7SIL-120
Wakosil 10SIL
L7 C8 Octylsilane chemically bonded to totally or superficially porous silica particles,
1.5 to 10 µm in diameter.
Wakosil-Ⅱ 3C8 RS
Wakosil 5C8
Wakosil-Ⅱ 5C8 HG
Wakosil-Ⅱ 5C8 RS
L8 NH2 Aminopropylsilane chemically bonded to totally porous silica gel support,
1.5 to 10 µm in diameter.
Wakosil 5NH2
L10 CN Nitrile groups chemically bonded to porous silica particles,
1.5 to 10 µm in diameter.
Combi CN
Wakosil 5CN
Wakosil-Ⅱ 5CN
L11 Ph Phenyl groups chemically bonded to porous silica particles,
1.5 to 10 µm in diameter.
Wakosil 5Ph
L13 C1 Trimethylsilane chemically bonded to porous silica particles,
3 to 10 µm in diameter.
Wakosil 5TMS
L20 Diol Dihydroxypropane groups chemically bonded to porous silica or hybrid particles,
1.5 to 10 µm in diameter.
Wakosil 5Diol-60
Wakosil 5Diol-120
Wakosil 5Diol-200
L26 C4 Butylsilane chemically bonded to totally porous silica particles, 1.5 to 10 µm in diameter. Wakosil 5C4
Wakosil 5C4-200
L33 Diol Packing having the capacity to separate proteins of 4 to 500 kDa.
It is spherical, silica-based and processed to provide pH stability.
Wakosil 5Diol-60
Wakosil 5Diol-120
Wakosil 5Diol-200
Wakosil 5Diol-300
L59 Diol Packing for the size-exclusion separations of proteins
(separation by molecular weight) over the range of 5 to 7,000 kDa.
Thepacking is spherical 1.5-10 µm, silica or hybrid packing with a hydrophilic coating.
Wakosil 5Diol-60
Wakosil 5Diol-120
Wakosil 5Diol-200
Wakosil 5Diol-300
L62 C30 C30 silane bonded phase on a fully porous spherical silica, 3-15 µm in diameter. Navi C30-5
L118 C18 Aqueous polymerized C18 groups on silica particles, 1.2 to 5 µm in diameter. Wakosil-PAHs
L## Polyfluoroalkyl (Ethylhexyl triazone, FluoFix)-Fluorocarbon chains chemically bonded to
5 µm spherical silica particles.
Fluofix-Ⅱ 120E

For research use or further manufacturing use only. Not for use in diagnostic procedures.

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