Wakopak® Fluofix®-II 120E
Fluofix® is a column packed with a carrier of high-purity spherical silica gel chemically modified with fluorinated silicone. As with hydrocarbon-based chemically modified packing agents (C18, C8, etc.), separation is basically performed in the reversed-phase distribution mode. However, the separation behavior is specific to each compound due to the strong water- and oil-repellent and rigid molecular structure of fluorinated silicon.
Features
- Easier application of basic compounds to LC/MS analysis
- Specific ability to recognize halogen atoms
- Shows excellent structural recognition with rigid structure
Essential Performance Test
- <Sample>
1. Uracil
2. Methyle Benzoate
3. Toluene
4. Triphenylene
5. o-Terphenyl
6. Hexafluorobenzene in CH3OHColumn size
Eluent
Flow rate
Detection
Injection:4.6 mm x 250 mm
:CH3OH/H2O=60/40 (v/v)
:1.0 mL/min. at 40℃
:UV 254 nm
:5 μL
Application
Analysis for fluorinated anilines
- <Sample>
1. p-Fluoroaniline
2. o-Fluoroaniline
3. m-FluoroanilineColumn size
Eluent
Flow rate
Detection
Injection:4.6 mm x 250 mm
:CH3OH/20 mmol/L TBABr*=20/80 (v/v)
:1.0 mL/min. at 40℃
:UV 254 nm
:10 μL
*: Tetrabutylammonium bromide
Analysis for fluorinated benzenes
- <Sample>
1. Uracil
2. Benzene
3. Fluorobenzene
4. p-Difluorobenzene
5. o-Difluorobenzene6. m-Difluorobenzene
7. 1, 3, 5-Trifluorobenzene
8. 1, 2, 3, 4-Tetrafluorobenzene
9. Pentafluorobenzene
10. Hexafluorobenzene in CH3OHColumn size
Eluent
Flow rate
Detection
Injection:4.6 mm x 250 mm
:CH3OH/H2O=60/40 (v/v)
:1.0 mL/min. at 40℃
:UV 254 nm
:10 μL
Analysis for fluorinated anisoles
- <Sample>
1. o-Fluoroanisole
2. p-Fluoroanisole
3. m-Fluoroanisole
4. 2, 4-Difluoroanisole in CH3OHColumn size
Eluent
Flow rate
Detection
Injection:4.6 mm x 250 mm
:CH3OH/H2O=60/40 (v/v)
:1.0 mL/min. at 40℃
:UV 254 nm
:10 μL
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