Wakopak® Wakosil®-Ⅱ 3C18 HG, RS, AR
- Completely porous, spherical packing agent with a diameter of 3 μm.
- The number of theoretical plates and resolution are improved compared with the 5 μm packing agent (≥100,000 theoretical plates/m). Therefore, it is possible to shorten the column.
- Line-up three types, HG, RS, and AR, as in the case of the 5C18 Series, and facilitate the migration of analytical conditions.
- Solvent usage is reduced, thus economizing on resources.
- Sample microsizing and high-sensitivity analysis are possible.
- Can be used in LC/MS.
Applications
Investigation of column shortening (analysis of preservative (paraben))
-
Sample
Eluent
Flow Rate
Detection：1）Methyl p-Hydroxybenzoate
2）Ethyl p-Hydroxybenzoate
3）Isopropyl p-Hydroxybenzoate
4）n-Propyl p-Hydroxybenzoate
5）sec-Butyl p-Hydroxybenzoate
6）Isobutyl p-Hydroxybenzoate
7）n-Butyl p-Hydroxybenzoate
：CH3OH/5 mM Citrate buffer（pH4.0）
：0.8 mL/min. at 40℃
：UV 270 nm
Features of semi-micro columns
Semi-micro columns with an inside diameter of 1.0 to 2.0 mm can be used at a lower flow rate than a general-purpose column to reduce the amount of solvent used. And semi-micro columns are suitable for use in LC/MS. In addition, analysis is possible with a small amount of sample by improved sensitivity. For example, when columns with inside diameters of 2.0 mm and 4.6 mm are compared, the ratio of cross-sectional areas is approximately 1/5; therefore, the sensitivity is increased by a factor of approximately 5, and the amount of solvent consumed is approximately 1/5.
Data comparison between 3 mm and 4.6 mm internal diameter columns
A general-purpose column with an internal diameter of 3.0 mm achieves a solvent consumption of approximately 1/2 and approximately double the sensitivity with the equipment available presently, in contrast to the equipment responding to the semi-micro column with the detector cell volume.
Example of shortened analysis time (Veterinary Drug Mixture Standard Solution PL-2-1: 24 species mixture, 20 μg/mL each of methanol solution)
Column: Wakopak® Wakosil®-Ⅱ 3C18 HG
positive mode 23 ingredients 0.1 ppm
negative mode 1 ingredients 0.1 ppm
HPLC conditionss
| Column Eluent |
：Wakopak® Wakosil®-Ⅱ 3C18 HG ：A）0.1％ HCOOH in H2O* B）0.1％ HCOOH in CH3CN** |
|||||||||||||||
| Time Program |
|
|||||||||||||||
| Flow Rate Injection Vol. |
：0.2 mL/min. at 40℃ ：0.1 ppm, 4 μL (3.0 x 150 mm), 3 μL (2.0 x 100 mm) |
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| * ** |
Formic Acid (abt. 99%) for LC/MS (Code No. 067-04531) Ultrapure Water for LC/MS (Code No. 214-01301) 0.1 vol% Formic Acid-Acetonitrile (Code No. 062-04721) |
MS/MS conditions
ESI, MRM
| Ion Spray Voltage Temperature Curtain Gas Collision Gas Ion Source Gas 1 Ion Source Gas 2 System |
：5500 V (pos), -4500 V (neg) ：400℃ (po9), 500℃ (neg) ：20 ：6 ：50 ：80 ：3200Q TRAP (ABI) |
Veterinary Drug Mixture Standard Solution PL-2-1: 24 species mixture
| Peak No. |
Analyte Peak Name | Analyte Mass Ranges (amu) |
Analyte Retention Time (min) |
|||
|---|---|---|---|---|---|---|
| Precursor | Product | Mode | 3 x 150 mm | 2 x 100 ｍｍ | ||
| 1 | 5-Propylsulfonyl-1H-benzimidazole-2-amine | 240.1 | 133.2 | + | 11.2 | 4.7 |
| 2 | Levamisole | 207.1 | 91.2 | + | 11.3 | 4.7 |
| 3 | Thiabendazole | 202.1 | 175.1 | + | 11.8 | 4.9 |
| 4 | Trimethoprim | 291.1 | 230.3 | + | 12.0 | 4.9 |
| 5 | Sulfadiazine | 251.1 | 92.2 | + | 12.3 | 5.0 |
| 6 | Ormetoprim | 275.2 | 123.1 | + | 12.5 | 5.1 |
| 7 | Sulfathiazole | 256.0 | 156.0 | + | 12.8 | 5.2 |
| 8 | Sulfapyridine | 250.0 | 156.2 | + | 13.2 | 5.3 |
| 9 | Sulfamerazine | 265.1 | 92.2 | + | 13.8 | 5.5 |
| 10 | Thiamphenicol | 355.9 | 308.0 | + | 14.4 | 5.7 |
| 11 | Sulfadimidine | 279.1 | 92.1 | + | 15.0 | 5.9 |
| 12 | Sulfamethoxypyridiazine | 281.1 | 156.1 | + | 15.0 | 5.9 |
| 13 | Sulfamonomethoxine | 281.1 | 156.1 | + | 16.1 | 6.2 |
| 14 | Sulfachlorpyridazine | 286.1 | 157.1 | + | 16.7 | 6.4 |
| 15 | Sulfamethoxazole | 254.0 | 156.0 | + | 17.5 | 6.6 |
| 16 | Sulfadoxine | 311.1 | 156.1 | + | 17.4 | 6.6 |
| 17 | Ethopabate | 238.2 | 136.0 | + | 19.3 | 7.2 |
| 18 | Sulfaquinoxaline | 301.0 | 156.2 | + | 19.4 | 7.2 |
| 19 | Sulfadimethoxine | 311.1 | 156.2 | + | 19.4 | 7.2 |
| 20 | Sulfanitran | 336.1 | 134.1 | + | 21.9 | 7.9 |
| 21 | β-Trenbolone | 271.2 | 165.3 | + | 23.2 | 8.3 |
| 22 | α-Trenbolone | 271.2 | 165.2 | + | 23.7 | 8.5 |
| 23 | Melengestrol Acetate | 397.2 | 279.4 | + | 31.3 | 10.7 |
| 1 | Zeranol | 321.1 | 277.2 | - | 24.2 | 8.7 |
Veterinary Drug Mixture Standard Solution PL-2-1 (Code No. 227-01691)
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Wakopak® Wakosil®-Ⅱ 3C18 HG
Wakopak® Wakosil®-Ⅱ 3C18 RS
Wakopak® Wakosil®-Ⅱ 3C18 AR
For research use or further manufacturing use only. Not for use in diagnostic procedures.
Product content may differ from the actual image due to minor specification changes etc.
If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.
The prices are list prices in Japan.Please contact your local distributor for your retail price in your region.