Reverse Phase Chromatography (C18)
Chromatography is the separation of an analyte (sample) between two immiscible phases stationary phase (packing agent) and mobile phase based on their partitioning equilibrium.
Among chromatography methods, reverse phase chromatography uses a packing agent with low polarity and a mobile phase with high polarity, while normal phase chromatography uses a packing agent with high polarity and a mobile phase with low polarity. Reverse phase chromatography often uses a packing agent chemically modified with alkyl chains on the silica gel surface, and octadecylsilyl modified silica gel (C18, ODS) are widely used for the packing agent.
Fujifilm Wako offers a wide variety of ODS columns with special characteristics.
More Information
How to Select an ODS Column
1. Select by physical properties of packing agent*1
2. Select by purpose*1
You can select the most suitable column by referring to the flowchart below.
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Highest priority
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Durability to eluents
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Separation performance
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For UHPLC
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Sharp peaks
(high number of theoretical plates) |
High retention
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3. Select by features*1
Features and applications of each column series are compared. Please make use of this comparison when selecting a column.
| Wakopak® Ultra C18 | Wakopak® Navi C18, C22, C30 | Wakosil®-II 5C18, 3C18 HG | Wakosil®-II 5C18, 3C18 RS | Wakosil®-II 5C18, 3C18 AR | Wakopak® Wakosil® Series | |
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| Features |
・Higher pressure resistance and base resistance than Wakosil®-II AR
・Available by UHPLC
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・Analysis by different alkyl chain lengths of C18, C22, and C30 is possible (C22 and C30 are highly stereoselective for compounds with similar structures)
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・High number of theoretical plates
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・High retention time
・High resolution in aqueous eluents
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・High pressure resistance, and high base resistance
・High stereoselectivity
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・Can be selected by end capping ratio and carbon content
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| Applications |
・Analysis using acidic and basic eluents
・Analysis using UHPLC
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・Analysis of analytes with similar structures
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・First choice for Wakopak® series
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・Analysis of analytes with a high range of polarity
・Separation of proximity peaks
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・Analysis using acidic and basic eluents
・Analysis of analytes with similar structures
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・Comparative verification of retention capacity and analysis time for different end-capping ratio and carbon content
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| Particle size | 2 μm, 3 μm, 5 μm | 5 μm | 3 μm, 5 μm | 3 μm, 5 μm | 3 μm, 5 μm | 5 μm, 7 μm, 10 μm |
| GMP analytical validation support*2 | - | - | ✓ | ✓ | ✓ | - |
| Prep series (for preparative chromatography)*2 | - | - | ✓ | ✓ | ✓ | - |
*1 Demo columns for our HPLC columns are available for loan. Please feel free to contact us.
*2 Please refer to the various links for overview of validation support and prep series.
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