Reverse Phase Chromatography (C18)

Chromatography is the separation of an analyte (sample) between two immiscible phases stationary phase (packing agent) and mobile phase based on their partitioning equilibrium.
Among chromatography methods, reverse phase chromatography uses a packing agent with low polarity and a mobile phase with high polarity, while normal phase chromatography uses a packing agent with high polarity and a mobile phase with low polarity. Reverse phase chromatography often uses a packing agent chemically modified with alkyl chains on the silica gel surface, and octadecylsilyl modified silica gel (C18, ODS) are widely used for the packing agent.

Fujifilm Wako offers a wide variety of ODS columns with special characteristics.

More Information

How to Select an ODS Column

1. Select by physical properties of packing agent*1

Reverse_Phase_Chromatography_img03.png

Click to enlarge

2. Select by purpose*1

You can select the most suitable column by referring to the flowchart below.

Highest priority
Durability to eluents
Separation performance
For UHPLC
Sharp peaks
(high number of theoretical plates)
High retention

3. Select by features*1

Features and applications of each column series are compared. Please make use of this comparison when selecting a column.

Wakopak® Ultra C18 Wakopak® Navi C18, C22, C30 Wakosil®-II 5C18, 3C18 HG Wakosil®-II 5C18, 3C18 RS Wakosil®-II 5C18, 3C18 AR Wakopak® Wakosil® Series
Features
・Higher pressure resistance and base resistance than Wakosil®-II AR
・Available by UHPLC
・Analysis by different alkyl chain lengths of C18, C22, and C30 is possible (C22 and C30 are highly stereoselective for compounds with similar structures)
・High number of theoretical plates
・High retention time
・High resolution in aqueous eluents
・High pressure resistance, and high base resistance
・High stereoselectivity
・Can be selected by end capping ratio and carbon content
Applications
・Analysis using acidic and basic eluents
・Analysis using UHPLC
・Analysis of analytes with similar structures
・First choice for Wakopak® series
・Analysis of analytes with a high range of polarity
・Separation of proximity peaks
・Analysis using acidic and basic eluents
・Analysis of analytes with similar structures
・Comparative verification of retention capacity and analysis time for different end-capping ratio and carbon content
Particle size 2 μm, 3 μm, 5 μm 5 μm 3 μm, 5 μm 3 μm, 5 μm 3 μm, 5 μm 5 μm, 7 μm, 10 μm
GMP analytical validation support*2 - - -
Prep series (for preparative chromatography)*2 - - -

*1 Demo columns for our HPLC columns are available for loan. Please feel free to contact us.
*2 Please refer to the various links for overview of validation support and prep series.  

For research use or further manufacturing use only. Not for use in diagnostic procedures.

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