for the Analysis of polar compounds etc.

Wakopak® Wakosil®-II C8 Series (5C8 HG, RS, 3C8 RS)

Wakosil®-II C8 Series uses a packing agent of high-purity silica gel chemically modified with an octyl (C8) group. As with Wakosil®-II C18 series, the raw material is high-purity silica gel with a low metal content, and it enables high-resolution analysis of basic compounds and coordination compounds.
Since the alkyl chain is shorter than ODS (C18) and the retention of highly hydrophobic compounds is lower, compounds with differences in polarity can be analyzed in a short time.
As with Wakosil®-II C18 series, there are two types: HG and RS.

Features

  • Compounds with differences in polarity can be analyzed in a short time.
  • A high-purity silica gel with a lower metal content was used.
  • Analytical validation is supported.

Applications

Essential performance test for comparison between C8 and C18 (ODS) packing agents

01233_img01.png
Sample


Eluent
Flow Rate
Detection
:1)Uracil
 2)Benzene
 3)Naphthalene
:CH3CN/H2O=60/40 (v/v)
:1.0 mL/min. at 35℃
:UV 254 nm
N=Plates for Last Peak,
K'=Capacity factor,
S=Assymetry factor (10% Height of Last Peak)

Comparison of retention behavior between C8 and C18 (ODS) packing agents

  • Analysis of aromatic hydrocarbons
    01233_img02.png
  •  
    Eluent
    Flow Rate
    Detection
    :CH3CN/H2O=60/40 (v/v)
    :1.0 mL/min. at 35℃
    :UV 254 nm 0.16 AUFS

 

  • Analysis of tricyclic antidepressants
    01233_img03.png
  •  
    Eluent
    Flow Rate
    Detection
    :CH3OH/20 mM KH2PO4,K2HPO4 (pH6.5)=62/38 (v/v)
    :1.0 mL/min. at 40℃
    :UV 254 nm

 

Example of comparison between Wakosil®-II 5C8 RS and 5C18 RS: Analysis of water-soluble vitamins

  • 01233_img04.png
  • Sample







    Eluent
    Flow Rate
    Detection
    Inj. Volume
    SHS
     1)L-Ascorbic acid (V.C.)
     2)Nicotinic acid
     3)Nicotinamide
     4)Pyridoxine・HCI (V.B6)
     5)Thiamine・HCI (V.B1)
     6)Caffeine
     7)D-Biotin (V.H)
     8)Riboflavin (V.B2)
    :CH3CN/0.1% H3PO4, 50 mM SHS=8/92 (v/v)
    :1.0 mL/min. at 40℃
    :UV 210 nm 0.128 AUFS
    :3 μL
    :Hexanesulfonic Acid Sodium Salt

    The analysis time can be shortened by using a C8 column without compromising the peak separation. This is particularly effective for analyzing highly hydrophobic compounds.

Product List

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Wakopak® Wakosil®-Ⅱ 5C8 HG

C8 column for reverse-phase chromatography. Compatible with analytical validation.

Wakopak® Wakosil®-Ⅱ 5C8 RS

C8 column for reverse-phase chromatography. Compatible with analytical validation.

Wakopak® Wakosil®-Ⅱ 3C8 RS

C8 column for reverse-phase chromatography. Compatible with analytical validation.

For research use or further manufacturing use only. Not for use in diagnostic procedures.

Product content may differ from the actual image due to minor specification changes etc.

If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.

The prices are list prices in Japan.Please contact your local distributor for your retail price in your region.

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