Wakopak® Wakosil®-II C8 Series (5C8 HG, RS, 3C8 RS)
Wakosil®-II C8 Series uses a packing agent of high-purity silica gel chemically modified with an octyl (C8) group. As with Wakosil®-II C18 series, the raw material is high-purity silica gel with a low metal content, and it enables high-resolution analysis of basic compounds and coordination compounds.
Since the alkyl chain is shorter than ODS (C18) and the retention of highly hydrophobic compounds is lower, compounds with differences in polarity can be analyzed in a short time.
As with Wakosil®-II C18 series, there are two types: HG and RS.
Features
- Compounds with differences in polarity can be analyzed in a short time.
- A high-purity silica gel with a lower metal content was used.
- Analytical validation is supported.
Applications
Essential performance test for comparison between C8 and C18 (ODS) packing agents
| Sample Eluent Flow Rate Detection |
：1）Uracil 2）Benzene 3）Naphthalene ：CH3CN/H2O=60/40 (v/v) ：1.0 mL/min. at 35℃ ：UV 254 nm |
| N=Plates for Last Peak, K'=Capacity factor, S=Assymetry factor (10% Height of Last Peak) |
Comparison of retention behavior between C8 and C18 (ODS) packing agents
-
Analysis of aromatic hydrocarbons
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Eluent
Flow Rate
Detection：CH3CN/H2O=60/40 (v/v)
：1.0 mL/min. at 35℃
：UV 254 nm 0.16 AUFS
-
Analysis of tricyclic antidepressants
-
Eluent
Flow Rate
Detection：CH3OH/20 mM KH2PO4,K2HPO4 (pH6.5)=62/38 (v/v)
：1.0 mL/min. at 40℃
：UV 254 nm
Example of comparison between Wakosil®-II 5C8 RS and 5C18 RS: Analysis of water-soluble vitamins
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Sample
Eluent
Flow Rate
Detection
Inj. Volume
SHS1）L-Ascorbic acid (V.C.)
2）Nicotinic acid
3）Nicotinamide
4）Pyridoxine・HCI (V.B6)
5）Thiamine・HCI (V.B1)
6）Caffeine
7）D-Biotin (V.H)
8）Riboflavin (V.B2)
：CH3CN/0.1% H3PO4, 50 mM SHS=8/92 (v/v)
：1.0 mL/min. at 40℃
：UV 210 nm 0.128 AUFS
：3 μL
：Hexanesulfonic Acid Sodium SaltThe analysis time can be shortened by using a C8 column without compromising the peak separation. This is particularly effective for analyzing highly hydrophobic compounds.
Product List
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Wakopak® Wakosil®-Ⅱ 5C8 HG
Wakopak® Wakosil®-Ⅱ 5C8 RS
Wakopak® Wakosil®-Ⅱ 3C8 RS
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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