High Molecular Amyloid β Oligomer ELISA Kit Wako Ver.2
- for Immunochemistry
- Manufacturer :
- FUJIFILM Wako Pure Chemical Corporation
- Storage Condition :
- Keep at 2-10 degrees C.
- Structural Formula
- Label
- Packing
- SDS
Comparison
|
Product Number
|
Package Size
|
Price
|
Inventory
|
||
---|---|---|---|---|---|---|
|
|
96Tests
|
|
In stock in Japan |
|
Document
Kit component
96 tests
Antibody (BAN50)-coated Plate | 1 plate |
---|---|
Standard Solution (10 nM) | 100 μL |
Buffer | 60 mL |
Biotin-conjugated Antibody(BAN50) Solution | 100 μL |
Peroxidase-conjugated Streptavidin Solution | 100 μL |
Luminescent Reagent 1 | 6 mL |
Luminescent Reagent 2 | 6 mL |
Wash Solution (10 x) | 100 mL |
Sample Buffer for Serum/Plasma | 50 mL |
Plate Seal | 4 sheets |
Overview
Features
- Detects in serum and plasma, CSF sample
- Specifically reactive with ≧9mer Aβ oligomer
Performance
-
Standard curve range 0.41-100 pM (Cerebrospinal fluid)
0.16-40 pM (serum or plasma(EDTA))Reactive Aβ oligomers ≧9mer Aβ oligomer Sample Serum, Plasma(EDTA),
Cerebrospinal fluid(CSF),
In vitro Aβ oligomerCross-reactivity Human Sample volume CSF:25μL(4 times dilution)
Serum, plasma:50μL(2 times dilution)Assay time 4.5 hours Detection method Luminescence method** - Calculated based on 16-mer MAP peptide.
- * Requires luminescence plate reader for measurement.
-
Assay principal
This ELISA kit is designed to measure ≧ 9-mer Aβ oligomer specifically by using same Aβ antibody (BAN50)
for capture and detection3)4). As a standard, a 16-mer MAP peptide with 16 epitopes of anti Aβ antibody (BAN50) is used4).
Specificity for Aβ oligomers in the ELISA
Aβ monomers were incubated the solution at 4℃ overnight in order to synthesize various size of Aβ oligomers. They were separated Fraction A and Fraction B by ultrafiltration. The fractions were analyzed by western blotting and the Aβ oligomer ELISA.
-
Western blotting using Aβ antibody(BAN50)
-
Aβ oligomer ELISA
A strong signal was exhibited in the fraction A containing 9-mer and more (>30 kDa), whereas few signal was detected in fraction B containing 8-mer and smaller (<30 kDa).
Example of measurements
Example of measurement 1 - spiked recovery test and dilution linearity -
Human plasma | Not spiked | +0.5pM Std. | +0.1pM Std. | +5pM Std. | +30pM Std. | ||||
---|---|---|---|---|---|---|---|---|---|
Assay value | Assay value | Recovery | Assay value | Recovery | Assay value | Recovery | Assay value | Recovery | |
1.85pM | 2.36pM | 102% | 2.85pM | 100% | 7.11pM | 105% | 31.2pM | 97.8% | |
Human serum | Not spiked | +0.5pM Std. | +0.1pM Std. | +5pM Std. | +30pM Std. | ||||
Assay value | Assay value | Recovery | Assay value | Recovery | Assay value | Recovery | Assay value | Recovery | |
1.58pM | 2.07pM | 98% | 2.59pM | 101% | 6.64pM | 101% | 31.9pM | 101% |
Human CSF1 | Not spiked | +3pM Std. | +6pM Std. | +15pM Std. | |||
---|---|---|---|---|---|---|---|
Assay value | Assay value | Recovery | Assay value | Recovery | Assay value | Recovery | |
5.16pM | 8.01pM | 95% | 10.6pM | 91% | 18.1pM | 86% | |
Human CSF2 | Not spiked | +3pM Std. | +6pM Std. | +15pM Std. | |||
Assay value | Assay value | Recovery | Assay value | Recovery | Assay value | Recovery | |
29.1pM | 38.8pM | 97% | 44.0pM | 99.3% | 59.9pM | 88% |
Example of measurement 2 - CSF sample -
Cerebrospinal fluids(CSF) of patients with Alzheimer disease (AD) and Mild Cognitive Impairment (MCI), non-dementia patients(control), were measured with our ELISA kit and competitor’s kit.
-
Age Sex MMSE※3 CSF
Aβ oligomer(pM)Control
n=569 M 30 2.37 60 F 30 1.65 59 M 30 2.82 46 M 28 2.24 60 F 29 1.34 MCI
n=569 F 24 3.6 76 M 23 2.62 82 M 26 2.33 69 F 23 2.67 73 F 25 2.34 AD
n=879 F 10 3.56 88 M 24 3.13 62 M 16 4.13 78 M 13 3.95 63 M 14 3.88 74 F 16 3.25 76 M 19 3.69 84 M 16 4.34 - ***MMSE score:diagnosis Inquiry test score of cognitive function
30-28 Normal
27-24 Suspected of mild cognitive impairment
≦23 Suspected of dementia
- ***MMSE score:diagnosis Inquiry test score of cognitive function
-
- There was a significant difference between Control, MCI and AD.
- High molecular Aβ oligomer correlated with MMSE score.
Property
Manufacturer Information
Alias
- Aβoligomer ELISA Kit
For research use or further manufacturing use only. Not for use in diagnostic procedures.
Product content may differ from the actual image due to minor specification changes etc.
If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.