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Detail Information
  1. Top
  2. Life Science
  3. Genetics
  4. microRNA
  5. Anti-Ago Monoclonal Antibodies
  6. Anti Ago1, Monoclonal Antibody(2A7)

Anti Ago1, Monoclonal Antibody(2A7)

for Immunochemistry
Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at 2-10 degrees C.
Host :
Mouse
Clone No. :
2A7
Classification :
Monoclonal
Citations (10)
PubMed Central® search
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SDS
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Product Number
Package Size
Price
Availability
Certificate of Analysis
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Distributor
015-22411
Barcode No
4987481488144
50uL
List Price
330.00 USD

In stock in Japan
Distributor

※Check  availability  in the US with the distributor.

Document

SDS
Product Specification Sheet
Package Insert
Spectral Data
Certificate of Analysis
Calibration Certificate

Overview / Applications

Outline This product is for research use only. Do not administer it to human.


Argonaute1(Ago1) was isolated as one of the component of RISC (RNA-induced silencing complex). Ago1 captures microRNA which are working as a guide molecule for interaction with target mRNAs in RNAi pathway. This monoclonal antibody is used for immunoprecipitation(IP) of Ago1 protein and microRNA interacted with Ago1.
Features:

  1. For Immunoprecipitation (IP)

  2. Cross reactivity with Ago1 protein of mouse and human

  3. For purification of RNA captured by RISC

  • Concentration (protein): Indicated on each label. (the 1st Lot.: 1.0 mg/mL)

  • The antibody contains 0.05 % Sodium Azide, 10 % Glycerol with 1 x TBS, pH 7.4.

  • Clone No.: 2A7

  • Subclass: IgG2a·κ

  • Antigen: Sythesized peptide of 19 N-terminal Ago1

  • Storage: Keep at 2 ~ 10 °C in the dark.

  • Avoid freezing and thawing.

    • [Working Dilution]




    ApplicationWorking Dilution
    Immunoprecipitation5 ~ 10 μg/20 μL 10% Protein G slurry

    [Reactivities]




    OrganismHumanMouse
    CellHeLaFM3A
    ImmunoprecipitationYesYes
    microRNA PurificationYesYes

    [Product List for microRNA Research]

































    Description
    Wako Cat. # (Pkg. Size)
    microRNA Purification kits based on Immunoprecipitation method using antibody against Argonaute protein
    microRNA Isolation Kit, Human/Mouse Ago1291-70201 (10 reactions)
    microRNA Isolation Kit, Human Ago2292-66701 (10 reactions)
    microRNA Isolation Kit, Mouse Ago2292-67301 (10 reactions)
    microRNA Isolation Kit, Human Ago3297-70301 (10 reactions)
    Preparation kits of cDNA encoding microRNA
    microRNA Cloning Kit Wako290-66501 (8 reactions)
    Single Strand DNA Ligase, thermostable, recombinant, Solution298-65103 (200 units); 292-65101 (500 units)
    Target mRNA Cloning Kit Wako298-67901 (30 reactions)
    PCR Purification Kit Wako298-68001 (10 reactions)
    THUNDER Taq Gold DNA Polymerase (containing Mg2+ free buffer)317-07081 (250 U)
    Antibodies
    Anti Ago1, Monoclonal Antibody (1F2) 018-22401 (50 μL)
    Anti Ago1, Monoclonal Antibody (2A7) 015-22411 (50 μL)
    Anti Human Ago2, Monoclonal Antibody (4G8)011-22033 (50 μL); 015-22031 (100 μL)
    Anti Mouse Ago2, Monoclonal Antibody (2D4) 014-22023 (50 μL); 018-22021 (100 μL)
    Anti Human Ago3, Monoclonal Antibody available soon!
    Features 1) For IP
    2) Cross reactivity with Ago1 protein of mouse and human
    3) For purification of RNA captured by RISC
    Instructions Immunoprecipitation:5-10ug/20uL 10% Protein G slurry
    Precautions for Use Even if some aggregates can be seen, quality of this antibody solution was confirmed in nomal use.

    Property

    Specificity Cross Reactivity:Human and mouse Ago1
    Composition 0.05% Sodium Azide, 10% Glycerol with 1xTBS, pH7.4
    Concentration Indicated on the label. (1st Lot. 1.0mg/mL)

    Antibody Information

    Antigen Ago1
    Antigen Alias Ago1
    EIF2C1
    Argonaute-1 (eIF2C1)
    eukaryotic translation initiation factor 2C, 1
    EIF2C1, AGO1 DKFZp686M13167 EIF2C GERP95 Q99
    AGO1/EIF2C1

    Manufacturer Information

    Alias

    • Anti Ago1, Monoclonal Antibody
      Anti Argonaute1, Monoclonal Antibody

    For research use or further manufacturing use only. Not for use in diagnostic procedures.

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