This site may not be displayed properly in the browser being used.
We recommend that you update to the latest browser.

It has been added to the cart.

It has been added to the comparison table.

Detail Information
  1. Top
  2. Life Science
  3. Genetics
  4. microRNA
  5. Anti-Ago Monoclonal Antibodies
  6. Anti Human Ago2, Monoclonal Antibody

Anti Human Ago2, Monoclonal Antibody

for Immunochemistry
Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at 2-10 degrees C.
Source :
Human
Host :
Mouse
Clone No. :
4G8
Classification :
Monoclonal
GHS :
Citations (33)
PubMed Central® search
  • Close
    Close
  • Close
    Close
  • Structural Formula
  • Label
  • Packing
SDS
Comparison
Product Number
Package Size
Price
Availability
Certificate of Analysis
Purchase
Distributor
011-22033
Barcode No
4987481480698
50uL
List Price
330.00 USD

In stock in Japan
Distributor
Distributor
015-22031
Barcode No
4987481480452
100uL
List Price
550.00 USD

In stock in Japan
Distributor

※Check  availability  in the US with the distributor.

Document

SDS
Product Specification Sheet
Package Insert
Spectral Data
Certificate of Analysis
Calibration Certificate

Overview / Applications

Outline This product is for research use only. Do not administer it to human.


Argonaute2 (AGO2) was isolated as one of the main components of RISC (RNA-induced silencing complex). AGO2 captures siRNA and microRNA which are working as a guide molecule for interaction with target mRNAs in RNAi pathway. In this pathway, AGO2 catalyzes the nicking of target mRNAs and binding between RISC and target mRNAs.
This monoclonal antibody is not only used for western blot and immunohistochemistry, but also immunoprecipitation of hAGO2.


Monoclonal Anti human AGO2 antibody is produced by hybridoma clone 4G8, established by fusing mouse myeloma cells P3U1 and lymphocytes of BALB/c mice immunized with recombinant human AGO2 produced in E. coli This antibody was purified by an affinity column from cell culture supernatant.


[Concentration] Indicated on each label.
[Formulation] 1 x TBS (pH 7.4) aqueous solution with 10% glycerol containing 0.09% sodium azide
[Clone No.] 4G8
[Specificity] Specific to human AGO2 [Subclass] IgG1
[Working Dilution]

  • Western blot 1:100 ~ 1:200

  • Immunoprecipitation 10 μg/20 μL 10% Protein G beads slurry

  • Immunocytochemistry 1:20 ~ 1:50

[Note] Avoid repeated freezing and thawing.
[Product List for microRNA Research]

































Description
Wako Cat. # (Pkg. Size)
microRNA Purification kits based on Immunoprecipitation method using antibody against Argonaute protein
microRNA Isolation Kit, Human/Mouse Ago1291-70201 (10 reactions)
microRNA Isolation Kit, Human Ago2292-66701 (10 reactions)
microRNA Isolation Kit, Mouse Ago2292-67301 (10 reactions)
microRNA Isolation Kit, Human Ago3297-70301 (10 reactions)
Preparation kits of cDNA encoding microRNA
microRNA Cloning Kit Wako290-66501 (8 reactions)
Single Strand DNA Ligase, thermostable, recombinant, Solution298-65103 (200 units); 292-65101 (500 units)
Target mRNA Cloning Kit Wako298-67901 (30 reactions)
PCR Purification Kit Wako298-68001 (10 reactions)
THUNDER Taq Gold DNA Polymerase (containing Mg2+ free buffer)317-07081 (250 U)
Antibodies
Anti Ago1, Monoclonal Antibody (1F2) 018-22401 (50 μL)
Anti Ago1, Monoclonal Antibody (2A7) 015-22411 (50 μL)
Anti Human Ago2, Monoclonal Antibody (4G8)011-22033 (50 μL); 015-22031 (100 μL)
Anti Mouse Ago2, Monoclonal Antibody (2D4) 014-22023 (50 μL); 018-22021 (100 μL)
Anti Human Ago3, Monoclonal Antibody available soon!
Features 1) For IP, Western Blot
2) Cross reactivity with AGO2 protein of rat and hamster
3) For purification of RNA captured by RISC

Property

Appearance Colorless liquid
Origin / Source This antibody was purified by an affinity column from cell culture supernatant.
Specificity Specific for human AGO2.
Composition 0.09% Sodium Azide, 10% Glycerol with TBS, pH7.4.
Concentration Indicated on the label.

Antibody Information

Antigen AGO2
Antigen Alias Ago 2
eukaryotic translation initiation factor 2C, 2
AGO2, MGC3183, Q10
EIF2C2
Eukaryotic Translation Initiation Factor 2C, 2 (EIF2C2)
Subclass IgG1

Manufacturer Information

Alias

  • Anti Human Argonaute 2, Monoclonal Antibody (4G8)

For research use or further manufacturing use only. Not for use in diagnostic procedures.

Product content may differ from the actual image due to minor specification changes etc.

If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.

Ask for help