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Anti Mouse Ago2, Monoclonal Antibody

for Immunochemistry
Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at 2-10 degrees C.
Source :
Mouse
Host :
Mouse
Clone No. :
2D4
Classification :
Monoclonal
GHS :
  • Structural Formula
  • Label
  • Packing
SDS
Comparison
Product Number
Package Size
Price
Availability
Certificate of Analysis
Purchase
Distributor
014-22023
Barcode No
4987481480629
50uL
List Price
330.00 USD

In stock in Japan

Distributor
018-22021
Barcode No
4987481480445
100uL
List Price
550.00 USD

In stock in Japan

Document

SDS
Product Specification Sheet
Package Insert
Spectral Data
Certificate of Analysis
Calibration Certificate

Overview / Applications

Outline This product is for research use only. Do not administer it to human.

Argonaute2 (AGO2) was isolated as one of the main components of RISC (RNA-induced silencing complex). AGO2 captures siRNA and microRNA, which are working as a guide molecule for interaction with target mRNAs in RNAi pathway. In this pathway, AGO2 catalyzes the nicking of target mRNAs and binding between RISC and target mRNAs.

This monoclonal antibody is not only used for western blot and immunohistochemistry, but also immunoprecipitation of mAGO2.

[Preparation]
Purified by an affinity column from mouse ascites and prepared in TBS (pH 7.4) aqueous solution with 10% glycerol containing 0.05% sodium azide.

[Antigen]
Synthetic partial peptides of mouse Ago2 protein coupled with bovine thyroglobulin (BTG)

[Concentration] Shown on each label
[Specificity]
React to mouse AGO2. Cross-react with AGO2 protein of rat and hamster.
Does not cross-react with human AGO2.
[Clone No.] 2D4
[Working Dilution]

  • Western blot: 1:200 ~ 1:1,000

  • Immunoprecipitation: 10 μg/20μL 10 % Protein G beads slurry

[Product List for microRNA Research]

































Description
Wako Cat. # (Pkg. Size)
microRNA Purification kits based on Immunoprecipitation method using antibody against Argonaute protein
microRNA Isolation Kit, Human/Mouse Ago1291-70201 (10 reactions)
microRNA Isolation Kit, Human Ago2292-66701 (10 reactions)
microRNA Isolation Kit, Mouse Ago2292-67301 (10 reactions)
microRNA Isolation Kit, Human Ago3297-70301 (10 reactions)
Preparation kits of cDNA encoding microRNA
microRNA Cloning Kit Wako290-66501 (8 reactions)
Single Strand DNA Ligase, thermostable, recombinant, Solution298-65103 (200 units); 292-65101 (500 units)
Target mRNA Cloning Kit Wako298-67901 (30 reactions)
PCR Purification Kit Wako298-68001 (10 reactions)
THUNDER Taq Gold DNA Polymerase (containing Mg2+ free buffer)317-07081 (250 U)
Antibodies
Anti Ago1, Monoclonal Antibody (1F2) 018-22401 (50 μL)
Anti Ago1, Monoclonal Antibody (2A7) 015-22411 (50 μL)
Anti Human Ago2, Monoclonal Antibody (4G8)011-22033 (50 μL); 015-22031 (100 μL)
Anti Mouse Ago2, Monoclonal Antibody (2D4) 014-22023 (50 μL); 018-22021 (100 μL)
Anti Human Ago3, Monoclonal Antibody available soon!
Features 1) For IP, Western Blot
2) Cross reactivity with AGO2 protein of rat and hamster
3) For purification of RNA captured by RISC

Property

Appearance Colorless liquid
Origin / Source This antibody was purified from mouse ascites.
Specificity Cross reactivity with AGO2 protein of rat and hamster.
Composition TBS (pH 7.4) aqueous solution with 10% glycerol containing 0.05% sodium azide.
Concentration Indicated on the label.

Antibody Information

Antigen AGO2
Antigen Alias Ago 2
eukaryotic translation initiation factor 2C, 2
AGO2, MGC3183, Q10
EIF2C2
Eukaryotic Translation Initiation Factor 2C, 2 (EIF2C2)
Subclass IgG1

Manufacturer Information

Alias

  • Anti Mouse Argonaute 2, Monoclonal Antibody (2D4)

For research use or further manufacturing use only. Not for use in diagnostic procedures.

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