Anti Ago1, Monoclonal Antibody(1F2)

for Immunochemistry

Manufacturer :: FUJIFILM Wako Pure Chemical Corporation

Storage Condition :: Keep at 2-10 degrees C.

Cross‒Reactivity :: Human

Host :: Mouse
Clone No. :: 1F2

Classification :: Monoclonal
Application :: WB

Structural Formula
Label
Packing

SDS

Comparison	Product Number	Package Size	Price	Inventory
	Distributor 018-22401 Barcode No 4987481488052	50uL	List Price 330.00 USD	Out of Stock Inquire	Certificate of Analysis

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Overview / Applications
Property
Antibody Information
Manufacturer Information
Alias
Related Information

Document

SDS

Product Specification Sheet

Package Insert

Spectral Data

Certificate of Analysis

50uL

Calibration Certificate

Overview / Applications

Outline

This product is for research use only. Do not administer it to
human.

Monoclonal Anti Ago1 (Argonaute 1) antibody is produced by hybridoma clone 1F2, established by fusing mouse myeloma cells P3U1 and lymphocytes of BALB/c mice immunized with synthetic partial peptides of Ago1 protein which was coupled with bovine thyroglobulin (BTG).
This antibody was purified from mouse ascites.

Subclass: IgG2a·κ
Clone No.: 1F2
Immunogen: Synthesized peptide of N terminal region of Ago1 protein
Concentration: Shown on each label
Preparation: 1x TBS (pH 7.4) aqueous solution with 10 % glycerol containing 0.05 % sodium azide.

[Application]
Western blot: 1 : 100 ~ 1 : 1,000
We recommend Anti Ago1 (Clone No. 2A7) (Wako Cat. #015-22411) for use as an antibody for immunoprecipitation.

[Specificity]
Recognize human and mouse Ago1
[Product List for microRNA Research]

Wako Catalog No.	Description	Package Size
microRNA Purification kits based on Immunoprecipitation method using antibody against Argonaute protein
microRNA Isolation Kit, Human/Mouse Ago1	291-70201 (10 reactions)
microRNA Isolation Kit, Human Ago2	292-66701 (10 reactions)
microRNA Isolation Kit, Mouse Ago2	292-67301 (10 reactions)
microRNA Isolation Kit, Human Ago3	297-70301 (10 reactions)
Preparation kits of cDNA encoding microRNA
microRNA Cloning Kit Wako	290-66501 (8 reactions)
Single Strand DNA Ligase, thermostable, recombinant, Solution	298-65103 (200 units); 292-65101 (500 units)
Target mRNA Cloning Kit Wako	298-67901 (30 reactions)
PCR Purification Kit Wako	298-68001 (10 reactions)
THUNDER Taq Gold DNA Polymerase (containing Mg²⁺ free buffer)	317-07081 (250 U)
Antibodies
Anti Ago1, Monoclonal Antibody (1F2)	018-22401 (50 μL)
Anti Ago1, Monoclonal Antibody (2A7)	015-22411 (50 μL)
Anti Human Ago2, Monoclonal Antibody (4G8)	011-22033 (50 μL); 015-22031 (100 μL)
Anti Mouse Ago2, Monoclonal Antibody (2D4)	014-22023 (50 μL); 018-22021 (100 μL)
Anti Human Ago3, Monoclonal Antibody	available soon!

Instructions

Western blot 1 : 100 - 1 : 1,000 We recommend 2A7(015-22411) for use as an antibody for immunoprecipitation.

Property

Specificity	Human and mouse Ago1
Composition	1xTBS (pH 7.4) aqueous solution with 10% glycerol containing 0.05% sodium azide.
Concentration	Indicated on the label.

Antibody Information

Antigen	Ago1
Antigen Alias	Ago1 EIF2C1 Argonaute-1 (eIF2C1) eukaryotic translation initiation factor 2C, 1 EIF2C1, AGO1 DKFZp686M13167 EIF2C GERP95 Q99 AGO1/EIF2C1
Subclass	IgG2a, κ

Manufacturer Information

Alias

Anti Ago1, Monoclonal Antibody
Anti Argonaute1, Monoclonal Antibody

Related Information

Product List

Product Name
Anti Ago1, Monoclonal Antibody(2A7)	for Immunochemistry
Anti Human Ago2, Monoclonal Antibody	for Immunochemistry
Anti Mouse Ago2, Monoclonal Antibody	for Immunochemistry
Anti Human Ago3, Monoclonal Antibody(1C12)	for Immunochemistry
Anti Mouse Ago3, Monoclonal Antibody(mA3-S9)	for Immunochemistry

For research use or further manufacturing use only. Not for use in diagnostic procedures.

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