Anti-Ago Monoclonal Antibody

FUJIFILM Wako offers a full line of mouse monoclonal antibodies both for Western blot detection and immunoprecipitation recognizing various human and mouse Ago-subfamily proteins. These tools allow selective immunoprecipitation of various Ago-subfamily proteins from human and mouse samples in order to obtain microRNA bonded to each. Proteins obtained can also be confi rmed with Western blot.

Immunoprecipitation of recombinant Ago proteins

We evaluated immunoprecipitation performance of each antibody using human and mouse recombinant Ago proteins (DYKDDDDK tag was fused to C-terminal). Each anti-Ago1 to anti-Ago4 antibody specifically immunoprecipitated each recombinant Ago protein, demonstrating that anti-Ago1 to anti-Ago4 antibodies are capable of immunoprecipitation of all Ago-subfamily proteins.

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[Detection]
Primary antibody: Anti-DYKDDDDK Antibody(1E6)
Secondary antibody: Anti-mouse IgG, Peroxidase Conjugated

Western blot analysis of recombinant Ago proteins

We evaluated Western blot performance of each antibody using human and mouse recombinant Ago proteins (DYKDDDDK tag was fused to C-terminal). Each anti-Ago1 to anti-Ago4 antibody specifically detected each recombinant Ago protein, demonstrating that anti- Ago1 to anti-Ago4 antibodies are capable of detection of all Ago-subfamily proteins.

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[Detection]
Primary antibody: Each Anti-Ago Monoclonal Antibody
Secondary antibody: Anti-mouse IgG, Peroxidase Conjugated

Endogenous Ago protein immunoprecipitation and Western blot analysis

We immunoprecipitated and then used Western blot analysis for detection of endogenous Ago proteins using anti-human Ago antibodies from human K562 cells and anti-mouse Ago antibodies from mouse P388D1 cells or mouse lung tissue. Each anti-Ago1 to anti-Ago4 antibody specifically immunoprecipitated each endogenous Ago protein and used for Western blot analysis, indicating that anti-Ago1 to anti- Ago4 antibodies are capable of immunoprecipitation of endogenous Ago1 to Ago3.

01158_img03.png
[Detection]
Primary antibody: Each Anti-Ago Monoclonal Antibody
Secondary antibody: Anti-mouse IgG, Peroxidase Conjugated

Product List

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Anti-Ago Monoclonal Antibody

Anti-Ago2 Monoclonal Antibody

Anti-Ago3 Monoclonal Antibody

Anti-Ago4 Monoclonal Antibody

Anti-Ago1-4 Monoclonal Antibody

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microRNA Isolation Kit

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