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LabAssay ™ Glucose

Manufacturer :
FUJIFILM Wako Shibayagi Corporation
Storage Condition :
Keep at 2-10 degrees C.
Molecular Weight :
0
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638-50971
Manufacturer
LABGLUC-M1
500Tests
List Price
235.00 USD

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Document

Package Insert
Spectral Data
Certificate of Analysis
Calibration Certificate

Kit component

Kit component

Buffer 150 mL x 1 vial
Chromogen Substrate for 150 mL x 1 vial
Standard Solution I 3 mL x 1 vial
Standard Solution II 3 mL x 1 vial

Overview

※For research purposes only with animal serum/plasma, Not for Diagnostic Use.

Introduction

Sugar is one of the most important sources of energy in biology. It is regulated by various factors within an organism. Glucose converges to a stable ratio of α-form and β-form in solutions.

Assay principle

α-D-Glucose is converted to β-D-Glucose by mutarotase. Hydrogen peroxide, which is produced by a reaction between β-D-Glucose and glucose oxidase(GOD), promotes oxidative condensation of phenol with 4-aminoantipyrine quantitatively. LabAssay™ Glucose is a kit used for the quantitative determination of glucose concentrations in samples by measuring absorbance of a red color which is generated by the oxidative condensation reaction.

Performance

Sample Serum, plasma(Mouse, Rat)
Standard curve range 50 ~ 500 mg/dL (mg/100 mL)
Measurement time approx. 10 min.
Amount of sample 2 μL
Measurement wavelength 505 nm(main), 600 nm(sub)

Standard Curve

04093493_img01.png

Procedure

Perform the assay in the wells according to the following table scheme.

Test Standard Blank
Chromogen reagent 300 μL 300 μL 300 μL
Sample 2 μL 2 μL - *1
Mix well and incubate at 37 ℃ for 5 min.
Measure the absorbance at 505 nm*2 of the test sample and standard solution with the blank solution as the control.

*1: The addition of water is omitted because the absorbance difference in the presence of the addition of 2 μL of water does not exhibit any practical use.
*2: Use 505 nm (Main) and 600 nm (Sub) for two wavelengths.

Data

Spike-and-Recovery Test

Spike and Recovery Test of Triglyceride, a known amount of standard is spiked into in mouse and rat sample n=2

Rat Sample Spike(mg/dL) Mean(mg/dL) Recovery(mg/dL) Recovery(%)
Serum - 96.1 - -
86.1 179 82.5 95.8
129 214 117 90.7
172 258 162 94.2
215 295 199 92.6
Plasma (EDTA) - 101 - -
95.0 193 92.0 96.8
143 226 125 87.4
190 273 172 90.5
238 310 209 87.8
Plasma (Heparin) - 103 - -
99.0 196 93.0 93.9
149 226 123 82.6
198 279 176 88.9
248 319 216 87.1

Mouse Sample Spike(mg/dL) Mean(mg/dL) Recovery(mg/dL) Recovery(%)
Serum - 98.1 - -
89.2 177 78.9 88.5
134 201 103 76.9
178 251 153 86.0
223 280 182 81.6
Plasma (EDTA) - 93 - -
90.8 174 81.0 89.2
136 203 110 80.9
182 248 155 85.2
227 276 183 80.6
Plasma (Heparin) - 111 - -
91.2 187 76.0 83.3
137 227 116 84.7
182 263 152 83.5
228 294 183 80.3

Linearity-of-Dilution Test

  • Rat serum
    04093493_img03.png
  • Mouse serum
    04093493_img04.png

Example of measurement

  • Nomal Rat serum, Sprague Dawley, male
    No. Measured(mg/dL) mean SD CV(%)
    1 132.2 139.9 136.1 5.4 4.0
    2 113.9 122.3 118.1 5.9 5.0
    3 135.2 143.8 139.5 6.1 4.3
    4 118.4 114.9 116.6 2.5 2.2
    5 132.0 136.9 134.5 3.5 2.6
  • Nomal Rat plasma K2EDTA, Sprague Dawley, male
    No. Measured(mg/dL) mean SD CV(%)
    1 107.3 108.2 107.8 0.64 0.59
    2 108.9 112.5 110.7 2.6 2.3
    3 124.3 123.4 123.9 0.65 0.52
    4 105.0 111.2 108.1 4.4 4.1
    5 110.2 118.5 114.3 5.9 5.1
  • Nomal Mouse serum, CD-1(ICR), male
    No. Measured(mg/dL) mean SD CV(%)
    1 127.1 132.6 129.9 3.9 3.0
    2 145.9 148.1 147.0 1.5 1.0
    3 143.4 158.4 150.9 11 7.0
    4 150.2 157.1 153.6 4.9 3.2
    5 136.3 132.2 134.3 2.9 2.1
  • Nomal Mouse plasma K2EDTA, CD-1(ICR), male
    No. Measured(mg/dL) mean SD CV(%)
    1 86.8 87.9 87.3 0.72 0.83
    2 88.3 94.4 91.3 4.4 4.8
    3 100.2 95.1 97.7 3.6 3.7
    4 107.7 98.8 103.2 6.3 6.1
    5 106.1 108.6 107.3 1.8 1.6
Nomal Mouse Whole Blood K2EDTA, CD(ICR), male
No. Measured(mg/dL) mean SD CV(%)
1 81.6 82.9 82.2 0.91 1.1
2 108 118 113 7.1 6.2
3 92.0 84.5 88.2 5.3 6.0
4 95.5 101 98.2 3.8 3.9
5 69.3 67.2 68.3 1.5 2.2
  • Nomal Canine serum,Beagle,male
    No. Measured(mg/dL) mean SD CV(%) Age(years)
    1 97.6 110 104 9.1 8.7 4
    2 71.1 79.7 75.4 6.1 8.1 6
    3 65.2 75.0 70.1 6.9 9.8 6
    4 63.3 62.7 63.0 0.39 0.61 11
    5 77.6 86.5 82.0 6.3 7.7 12
    6 73.3 79.0 76.2 4.0 5.3 6
    7 36.8 38.8 37.8 1.4 3.8 10
    8 56.2 60.2 58.2 2.8 4.9 4
  • Nomal Canine plasma EDTA,Beagle,male
    No. Measured(mg/dL) mean SD CV(%) Age(years)
    1 97.4 89.3 93.4 5.7 6.1 4
    2 96.0 90.9 93.5 3.6 3.9 6
    3 88.5 81.4 84.9 5.0 5.9 10
    4 78.6 71.8 75.2 4.8 6.4 12
    5 85.5 80.2 82.9 3.8 4.5 6
    6 75.0 71.8 73.4 2.2 3.0 11
    7 85.7 83.7 84.7 1.4 1.6 10
    8 83.9 82.6 83.2 0.91 1.1 12
  • Nomal Feline serum, Short hair, male
    No. Measured(mg/dL) mean SD CV(%) Age(years)
    1 63.2 64.7 64.0 1.1 1.7 3.0
    2 65.2 67.1 66.1 1.3 2.0 3.0
    3 32.4 37.0 34.7 3.2 9.4 6.0
    4 40.2 43.3 41.8 2.2 5.3 4.0
    5 44.8 44.2 44.5 0.46 1.0 6.0
    6 21.4 20.3 20.8 0.72 3.4 6.0
    7 253 248 250 3.7 1.5 1.0
    8 98.2 91.6 94.9 4.7 5.0 1.0
  • Nomal Feline plasma EDTA, Short hair, male
    No. Measured(mg/dL) mean SD CV(%) Age(years)
    1 200 201 200 0.94 0.47 0.5
    2 118 115 117 2.3 2.0 1.0
    3 223 224 224 0.54 0.24 3.0
    4 272 261 266 7.5 2.8 0.5
    5 120 119 119 0.83 0.69 3.0
    6 115 112 114 2.0 1.8 3.0
    7 134 136 135 1.4 1.0 0.5
    8 107 109 108 1.5 1.4 1.0

References

  1. Yamashita, Y. et al. : Biosci. Biotechnol. Biochem., 77, 888 (2013). *Mouse plasma
  2. Narita, T. et al. : Exp. Gerontol., 104, 127 (2018). *Rat plasma
  3. Yamasaki, M. et al. : Food Sci. Technol. Res., 21, 827 (2015). *Mouse serum
  4. Fan, Y. et al. : J. Biomed. Sci., 23, 56 (2016). *Mouse serum
  5. Wang, W. et al. : J. Renin Angiotensin Aldosterone Syst., 15, 384 (2014). *Mouse serum
  6. Yamashita, Y. et al. : J. Nutr. Sci., 1, e2 (2012). *Mouse plasma
  7. Maesako, M. et al. : Neurobiol. Aging., 33, 1011 (2012). *Mouse serum

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