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Detail Information
  1. Top
  2. Life Science
  3. Metabolism Research
  4. ELISA / Assay Kits (Metabolism)
  5. Creatinine Assay Kits
  6. LabAssay (TM) Creatinine

LabAssay (TM) Creatinine

for Cellbiology
Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at 2-10 degrees C.
GHS :
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SDS
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Product Number
Package Size
Price
Availability
Certificate of Analysis
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Distributor
291-93901
Barcode No
4548995102515
500Tests
List Price
330.00 USD

In stock in Japan
Distributor

※Check  availability  in the US with the distributor.

Document

SDS
Product Specification Sheet
Package Insert
Spectral Data
Certificate of Analysis
Calibration Certificate

Kit component

Kit component

Deproteinizing Reagent 150 mL x 1 bottle
Picric Acid Reagent (Picric Acid 22 mmol/L) 50 mL x 1 bottle
0.75 mol/L Sodium Hydroxide Solution 50 mL x 1 bottle
Standard Solution (Creatinine 10 mg/dL) 15 mL x 1 bottle

Overview

※For research purposes only with animal serum/plasma, Not for Diagnostic Use.

Introduction

Creatinine is a metabolite which is produced by creatine phosphate directly or dehydration of creatine in muscle and nerve. It is excreted from the body through kidney glomerular filtration.

Assay principle

LabAssay™ Creatinine can be used to measure the creatinin levels in samples by Jaffe's reaction where creatinine produces quantitatively an orange color with picric acid in alkaline condition.

Performance

Sample Serum, Plasma, Urine
Target animals Mouse, Rat, Cat, Dog
Standard curve range 2.5 - 10mg/mL (mg/100 mL)
Measurement time Approx. 40 min.
Amount of sample 50 μL
Measurement wavelength 520 nm

Standard Curve

04093736_img01.png

Procedure

Perform the assay in the wells according to the following table scheme.

Test Standard Blank
First operations should be performed in the sample tubes.
Sample Sample
50 μL		 Standard solution
50 μL		 Water
50 μL
Deproteinizing reagent 300 μL 300 μL 300 μL
Mix well, and stand at room temperature for 10 min.
Centrifuge over 2500 rpm for 10 min.
Subsequent operations should be performed in the wells.
Supernatant 100 μL 100 μL 100 μL
　 Stand at 25 ℃ - 30 ℃
Picric Acid Reagent 50 μL 50 μL 50 μL
0.75 mol/L Sodium Hydroxide Solution 50 μL 50 μL 50 μL
　 Stand at 25 ℃ - 30 ℃ for 20 min.
Measure the absorbance of the test sample and standard solution
at 520nm with the blank solution as the control within the next 30 min.

Data

Spike-and-Recovery Test

Spike and Recovery Test of Creatinine, a known amount of standard is spiked into in mouse and rat sample n=2.

Rat Sample Spike (mg/dL) Mean (mg/dL) Recovery (mg/dL) Recovery (%)
Serum - 2.86 - -
3.27 5.61 2.75 84.1
5.60 8.13 5.27 94.1
7.35 9.34 6.48 88.2
Plasma
(EDTA)		 - 2.78 - -
2.79 5.22 2.44 87.5
4.78 8.3 5.52 115
6.27 9.22 6.44 103
Plasma
(Heparin)		 - 2.88 - -
3.17 5.53 2.65 83.6
5.43 8.25 5.37 98.9
7.13 9.27 6.39 89.6
Mouse Sample Spike (mg/dL) Mean (mg/dL) Recovery (mg/dL) Recovery (%)
Serum - 2.68 - -
3.00 5.90 3.22 107
5.14 8.08 5.40 105
6.74 8.90 6.22 92.3
Plasma
(EDTA)		 - 3.14 - -
2.71 5.63 2.49 91.9
4.65 7.74 4.60 98.9
6.10 8.25 5.11 83.8
Plasma
(Heparin)		 - 3.01 - -
2.85 5.48 2.47 86.7
4.88 7.74 4.73 96.9
6.41 8.60 5.59 87.2

Linearity-of-Dilution Test

  • Rat sample
    04093736_img03.png
  • Mouse sample
    04093736_img04.png

Example of measurement

  • Normal Rat serum, Sprague Dawley, male
    No. Measured(mg/dL) mean SD CV(%)
    1 0.553 0.546 0.550 0.0049 0.90
    2 0.716 0.824 0.770 0.076 9.9
    3 1.12 1.26 1.19 0.095 8.0
    4 1.22 1.29 1.25 0.050 4.0
    5 1.04 0.994 1.01 0.029 2.9
  • Normal Rat plasma K2EDTA, Sprague Dawley, male
    No. Measured(mg/dL) mean SD CV(%)
    1 0.319 0.288 0.304 0.022 7.2
    2 0.638 0.627 0.633 0.0078 1.2
    3 0.824 0.871 0.848 0.033 3.9
    4 0.661 0.681 0.671 0.014 2.1
    5 0.205 0.233 0.219 0.020 9.0
  • Normal Mouse serum, CD-1(ICR), male
    No. Measured(mg/dL) mean SD CV(%)
    1 0.199 0.205 0.202 0.0042 2.1
    2 0.492 0.507 0.500 0.011 2.1
    3 0.734 0.722 0.728 0.0085 1.2
    4 0.641 0.716 0.679 0.053 7.8
    5 0.192 0.219 0.206 0.019 9.3
  • Normal Mouse plasma K2EDTA, CD-1(ICR), male
    No. Measured(mg/dL) mean SD CV(%)
    1 0.0950 0.103 0.0990 0.0057 5.7
    2 0.607 0.559 0.583 0.034 5.8
    3 0.556 0.528 0.542 0.020 3.7
    4 0.490 0.546 0.518 0.040 7.6
    5 0.113 0.110 0.112 0.0021 1.9
  • Normal Canine serum, Beagle, male
    No. Measured(mg/dL) mean SD CV(%) Age(years)
    1 1.67 1.48 1.57 0.13 8.4 4
    2 1.16 1.07 1.11 0.063 5.7 6
    3 1.54 1.41 1.48 0.092 6.2 6
    4 1.58 1.40 1.49 0.13 8.9 11
    5 1.46 1.49 1.47 0.023 1.6 12
    6 1.17 1.20 1.19 0.027 2.3 6
    7 1.16 1.20 1.18 0.027 2.3 10
    8 1.23 1.30 1.27 0.047 3.7 4
  • Normal Canine plasma EDTA, Beagle, male
    No. Measured(mg/dL) mean SD CV(%) Age(years)
    1 1.48 1.45 1.46 0.021 1.5 4
    2 1.21 1.18 1.20 0.024 2.0 6
    3 1.33 1.22 1.27 0.071 5.6 10
    4 1.56 1.49 1.53 0.048 3.2 12
    5 1.31 1.29 1.30 0.0099 0.76 6
    6 1.04 1.04 1.04 0.0028 0.27 11
    7 1.14 1.16 1.15 0.013 1.2 10
    8 1.26 1.29 1.28 0.019 1.5 12
  • Normal Feline serum, Short hair, male
    No. Measured(mg/dL) mean SD CV(%) Age(years)
    1 1.86 1.78 1.82 0.060 3.3 3.0
    2 1.58 1.53 1.55 0.034 2.2 3.0
    3 1.81 1.68 1.75 0.092 5.3 6.0
    4 2.17 1.95 2.06 0.16 7.6 4.0
    5 1.88 1.92 1.90 0.030 1.6 6.0
    6 1.89 1.86 1.87 0.017 0.91 6.0
    7 1.71 1.76 1.73 0.040 2.3 1.0
    8 1.48 1.56 1.52 0.055 3.6 1.0
  • Normal Feline plasma EDTA, Short hair, male
    No. Measured(mg/dL) mean SD CV(%) Age(years)
    1 1.63 1.61 1.62 0.011 0.65 0.5
    2 1.41 1.36 1.39 0.038 2.8 1.0
    3 1.78 1.62 1.70 0.11 6.7 3.0
    4 1.97 1.76 1.87 0.15 7.9 0.5
    5 1.66 1.67 1.66 0.0085 0.51 3.0
    6 1.28 1.29 1.29 0.0028 0.22 3.0
    7 1.26 1.30 1.28 0.029 2.3 0.5
    8 1.60 1.71 1.65 0.080 4.8 1.0

References

  1. Kawamoto, T. et al. : Glycative Stress Res., 3, 15 (2016). Relationships between urinary melatonin metabolites and glycative stress and body functional age *Human urine
  2. Guan, Y. et al. : J. Pharmacol. Sci., 135, 81 (2017). A protease-activated receptor-1 antagonist protects against podocyte injury in a mouse model of nephropathy *Mouse urine and plasma
  3. Ito, K. et al. : Biol. Pharm. Bull., 38, 1169 (2015). Increased Milk Protein Concentration in a Rehydration Drink Enhances Fluid Retention Caused by Water Reabsorption in Rats *Rat urine and plasma
  4. Tahara, Y. et al. : Med. Chem. commun., 8, 415 (2017). Encapsulation of a nitric oxide donor into a liposome to boost the enhanced permeation and retention (EPR) effect *Mouse Serum
  5. Nasrin, S. et al. : J. Pharmacol. Sci., 122, 270 (2013). Beneficial Effects of Gosha-jinki-gan and Green Tea Extract in Rats With Chemical Cystitis *Rat urine
  6. Toyama, K. et al. : Br. J. Pharmacol., 166, 1183 (2012). Loss of multidrug and toxin extrusion 1 (MATE1) is associated with metformin-induced lactic acidosis *Mouse plasma

Overview / Applications

Outline Creatinine is a metabolite which is produced by creatine phosphate directly or dehydration of creatine in muscle and nerve. It is excreted from the body through kidney glomerular filtration. This product can be used to measure the creatinin levels in samples by Jaffe's reaction where creatinine produces quantitatively an orange color with picric acid in alkaline condition.

Property

Manufacturer Information

Alias

  • 636-51011 (Previous code)
    LABCREA-M1 (Previous code)
    290-65901 (Previous code)

For research use or further manufacturing use only. Not for use in diagnostic procedures.

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