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LabAssay ™ Triglyceride

Manufacturer :
FUJIFILM Wako Shibayagi Corporation
Storage Condition :
Keep at 2-10 degrees C.
Molecular Weight :
0
GHS :
  • Structural Formula
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Product Number
Package Size
Price
Inventory
Distributor
632-50991
Manufacturer
LABTRIG-M1
350Tests
List Price
235.00 USD

In stock

Document

Package Insert
Spectral Data
Certificate of Analysis
Calibration Certificate

Kit component

Kit Contents

Buffer 105 mL x 1 vial
Chromogen Substrate for 105 mL x 1 vial
Standard Solution 4 mL x 1 vial

Overview

※For research purposes only with animal serum/plasma, Not for Diagnostic Use.

Introduction

Triglycerides are neutral fats consisting of three fatty acids esterified to a glycerol backbone. There are triglycerides, cholesterol, phospholipids, free fatty acids and fat-soluble vitamins as lipid-soluble substances in the blood.

Assay principle

Triglycerides are converted to glycerol-3-phosphate by lipoprotein lipase and glycerolkinase. Hydrogen peroxide, which is produced by a reaction between the glycerol-3-phosphate and glycerol-3-phosphate oxidase(GPO), promotes oxidative condensation of N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethoxyaniline sodium salt(DAOS) with 4-aminoantipyrine. LabAssay™ Triglyceride can be used to detect triglycerides concentration in samples by measuring absorbance of a blue color which is generated by the oxidative condensation reaction.

Procedure

Perform the assay in the wells according to the following table scheme.

Test Standard Blank
Chromogen reagent 300 μL 300 μL 300 μL
Sample Serum 2 μL Standard solution 2 μL -
Mix well and incubate at 37 ℃ for 5 min. Measure the absorbance at 600 nm*1 of the test sample and standard solution with the blank solution as the control.

*1: In two wavelength assay, measure using Main wavelength 600 nm/Sub wavelength 700 nm.

Data

Performance

Sample Serum, plasma(Mouse, Rat)
Standard curve range 100 - 888 mg/dL (mg/100 mL)
Measurement time Approx. 10 min.
Amount of sample 2 μL
Measurement wavelength 600 nm(Main), 700 nm(Sub)

Standard Curve

04093432_img01.png

Spike-and-Recovery Test

Spike and Recovery Test of Triglyceride, a known amount of standard is spiked into in mouse and rat sample n=2.

Rat Sample Spike(mg/dL) Mean(mg/dL) Recovery(mg/dL) Recovery(%)
Serum - 130 - -
131 257 127 97.0
196 307 177 90.3
262 370 240 91.6
327 413 283 86.5
Plasma
(EDTA)
- 128 - -
135 256 128 94.8
203 312 183 90.1
271 374 245 90.4
338 401 272 80.5
Plasma
(Heparin)
- 123 - -
134 257 134 100
201 305 182 90.5
268 365 242 90.3
335 400 278 83.0

 

Mouse Sample Spike(mg/dL) Mean(mg/dL) Recovery(mg/dL) Recovery(%)
Serum - 133 - -
129 268 136 105
194 342 209 108
258 409 276 107
323 448 315 97.5
Plasma
(EDTA)
- 139 - -
129 288 149 116
193 342 203 105
258 409 270 105
322 446 307 95.3
Plasma
(Heparin)
- 151 - -
132 292 141 107
198 349 198 100
264 423 272 103
329 442 291 88.4

Linearity-of-Dilution Test

Rat serum

04093432_img03R.png
  • Mouse serum

    04093432_img04R.png

Example of measurement

  • Nomal Rat serum, Sprague Dawley, male
    No. Measured(mg/dL) mean SD CV(%)
    1 54.5 53.1 53.8 1.0 1.9
    2 153 153 153 0.41 0.27
    3 105 95.8 100 6.4 6.3
    4 120 125 123 4.1 3.4
    5 102 107 104 3.7 3.5
  • Nomal Rat plasma K2EDTA, Sprague Dawley, male
    No. Measured(mg/dL) mean SD CV(%)
    1 56.8 64.3 60.6 5.3 8.8
    2 73.2 67.2 70.2 4.3 6.1
    3 95.1 85.5 90.3 6.8 7.5
    4 68.5 63.8 66.2 3.3 5.0
    5 76.2 68.9 72.5 5.1 7.1
  • Nomal Mouse serum, CD-1(ICR), male
    No. Measured(mg/dL) mean SD CV(%)
    1 80.8 77.9 79.3 2.0 2.6
    2 78.4 76.7 77.6 1.2 1.6
    3 81.3 75.6 78.4 4.1 5.2
    4 57.4 60.3 58.8 2.0 3.5
    5 48.7 53.3 51.0 3.3 6.4
  • Nomal Mouse plasma K2EDTA, CD-1(ICR), male
    No. Measured(mg/dL) mean SD CV(%)
    1 28.8 31.7 30.2 2.0 6.7
    2 132 145 139 9.5 6.9
    3 62.7 68.0 65.4 3.8 5.8
    4 29.5 25.8 27.6 2.6 9.5
    5 58.8 55.1 56.9 2.7 4.7
  • Nomal Canine plasma EDTA,Beagle,male
    No. Measured(mg/dL) mean SD CV(%) Age(years)
    1 37.2 33.9 35.5 2.3 6.5 4
    2 29.0 27.6 28.3 1.0 3.6 6
    3 32.5 36.0 34.2 2.5 7.4 10
    4 33.5 36.4 34.9 2.1 5.9 12
    5 33.2 30.3 31.8 2.1 6.6 6
    6 24.9 22.9 23.9 1.4 6.0 11
    7 31.9 28.4 30.2 2.5 8.2 10
    8 63.2 57.0 60.1 4.36 7.3 12
  • Nomal Canine serum,Beagle,male
    No. Measured(mg/dL) mean SD CV(%) Age(years)
    1 35.6 39.7 37.7 2.9 7.7 4
    2 33.8 37.0 35.4 2.2 6.3 6
    3 28.6 31.0 29.8 1.7 5.7 6
    4 102 109 105 4.6 4.4 11
    5 37.3 34.2 35.8 2.2 6.0 12
    6 25.3 24.4 24.8 0.68 2.7 6
    7 18.6 16.5 17.5 1.4 8.1 10
    8 47.7 42.7 45.2 3.5 7.9 4
  • Nomal Feline plasma EDTA,Short hair,male
    No. Measured(mg/dL) mean SD CV(%) Age(years)
    1 104 104 104 0.21 0.20 0.5
    2 34.3 31.8 33.1 1.7 5.3 1.0
    3 54.8 49.3 52.1 3.9 7.5 3.0
    4 65.1 60.7 62.9 3.1 5.0 0.5
    5 43.9 44.4 44.1 0.41 0.94 3.0
    6 29.0 31.9 30.5 2.1 6.7 3.0
    7 16.8 16.2 16.5 0.4 2.5 0.5
    8 25.0 27.2 26.1 1.5 5.8 1.0
  • Nomal Feline serum,Short hair,male
    No. Measured(mg/dL) mean SD CV(%) Age(years)
    1 32.4 35.9 34.1 2.5 7.3 3.0
    2 18.4 20.2 19.3 1.3 6.7 3.0
    3 33.9 36.6 35.3 1.9 5.4 6.0
    4 48.9 53.8 51.3 3.5 6.8 4.0
    5 33.9 32.8 33.4 0.82 2.5 6.0
    6 45.3 47.1 46.2 1.24 2.7 6.0
    7 96.6 88.0 92.3 6.1 6.6 1.0
    8 52.3 54.7 53.5 1.7 3.1 1.0

References

  1. Gao, F. et al. : Evid. Based Complement. Alternat. Med., 2015, 801291 (2015). *Extraction liquid of rat liver
  2. Funakoshi, T. et al. : Biochem. Biophys. Rep., 13, 39 (2018). *Rat muscle satellite cell
  3. Moser, V. A. and Pike, C. J. : eNeuro, 4, e0077-17 (2017). *Rat plasma
  4. Fujii, N. et al. : Aging Cell, 16, 508 (2017). *Rat plasma
  5. Wang, F. et al. : J. Mol. Endocrinol., 52, 133 (2014). *Rat plasma
  6. Kato, H. et al. : J. Hepatol., 60, 1032 (2014). *Extraction liquid of mouse liver
  7. Fujimori, K. et al. : Prostaglandins. Other. lipid. Mediat., 94, 96 (2011). *Extraction liquid of mouse culture cells

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