LabAssay (TM) Triglyceride
- Manufacturer :
- FUJIFILM Wako Shibayagi Corporation
- Storage Condition :
- Keep at 2-10 degrees C.
- GHS :
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- Structural Formula
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350Tests
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Document
Kit component
Kit Contents
| Buffer | 105 mL x 1 vial |
|---|---|
| Chromogen Substrate | for 105 mL x 1 vial |
| Standard Solution | 4 mL x 1 vial |
Overview
※For research purposes only with animal serum/plasma, Not for Diagnostic Use.
Introduction
Triglycerides are neutral fats consisting of three fatty acids esterified to a glycerol backbone. There are triglycerides, cholesterol, phospholipids, free fatty acids and fat-soluble vitamins as lipid-soluble substances in the blood.
Assay principle
Triglycerides are converted to glycerol-3-phosphate by lipoprotein lipase and glycerolkinase. Hydrogen peroxide, which is produced by a reaction between the glycerol-3-phosphate and glycerol-3-phosphate oxidase(GPO), promotes oxidative condensation of N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethoxyaniline sodium salt(DAOS) with 4-aminoantipyrine. LabAssay™ Triglyceride can be used to detect triglycerides concentration in samples by measuring absorbance of a blue color which is generated by the oxidative condensation reaction.
Procedure
Perform the assay in the wells according to the following table scheme.
| Test | Standard | Blank | |
|---|---|---|---|
| Chromogen reagent | 300 μL | 300 μL | 300 μL |
| Sample | Serum 2 μL | Standard solution 2 μL | - |
| Mix well and incubate at 37 ℃ for 5 min. Measure the absorbance at 600 nm*1 of the test sample and standard solution with the blank solution as the control. | |||
*1: In two wavelength assay, measure using Main wavelength 600 nm/Sub wavelength 700 nm.
Data
Performance
| Sample | Serum, plasma(Mouse, Rat) |
|---|---|
| Standard curve range | 100 - 888 mg/dL (mg/100 mL) |
| Measurement time | Approx. 10 min. |
| Amount of sample | 2 μL |
| Measurement wavelength | 600 nm(Main), 700 nm(Sub) |
Standard Curve
Spike-and-Recovery Test
Spike and Recovery Test of Triglyceride, a known amount of standard is spiked into in mouse and rat sample n=2.
| Rat Sample | Spike(mg/dL) | Mean(mg/dL) | Recovery(mg/dL) | Recovery(%) |
|---|---|---|---|---|
| Serum | - | 130 | - | - |
| 131 | 257 | 127 | 97.0 | |
| 196 | 307 | 177 | 90.3 | |
| 262 | 370 | 240 | 91.6 | |
| 327 | 413 | 283 | 86.5 | |
| Plasma (EDTA) |
- | 128 | - | - |
| 135 | 256 | 128 | 94.8 | |
| 203 | 312 | 183 | 90.1 | |
| 271 | 374 | 245 | 90.4 | |
| 338 | 401 | 272 | 80.5 | |
| Plasma (Heparin) |
- | 123 | - | - |
| 134 | 257 | 134 | 100 | |
| 201 | 305 | 182 | 90.5 | |
| 268 | 365 | 242 | 90.3 | |
| 335 | 400 | 278 | 83.0 |
| Mouse Sample | Spike(mg/dL) | Mean(mg/dL) | Recovery(mg/dL) | Recovery(%) |
|---|---|---|---|---|
| Serum | - | 133 | - | - |
| 129 | 268 | 136 | 105 | |
| 194 | 342 | 209 | 108 | |
| 258 | 409 | 276 | 107 | |
| 323 | 448 | 315 | 97.5 | |
| Plasma (EDTA) |
- | 139 | - | - |
| 129 | 288 | 149 | 116 | |
| 193 | 342 | 203 | 105 | |
| 258 | 409 | 270 | 105 | |
| 322 | 446 | 307 | 95.3 | |
| Plasma (Heparin) |
- | 151 | - | - |
| 132 | 292 | 141 | 107 | |
| 198 | 349 | 198 | 100 | |
| 264 | 423 | 272 | 103 | |
| 329 | 442 | 291 | 88.4 |
Linearity-of-Dilution Test
Rat serum
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Mouse serum
Example of measurement
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Nomal Rat serum, Sprague Dawley, male No. Measured(mg/dL) mean SD CV(%) 1 54.5 53.1 53.8 1.0 1.9 2 153 153 153 0.41 0.27 3 105 95.8 100 6.4 6.3 4 120 125 123 4.1 3.4 5 102 107 104 3.7 3.5 -
Nomal Rat plasma K2EDTA, Sprague Dawley, male No. Measured(mg/dL) mean SD CV(%) 1 56.8 64.3 60.6 5.3 8.8 2 73.2 67.2 70.2 4.3 6.1 3 95.1 85.5 90.3 6.8 7.5 4 68.5 63.8 66.2 3.3 5.0 5 76.2 68.9 72.5 5.1 7.1
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Nomal Mouse serum, CD-1(ICR), male No. Measured(mg/dL) mean SD CV(%) 1 80.8 77.9 79.3 2.0 2.6 2 78.4 76.7 77.6 1.2 1.6 3 81.3 75.6 78.4 4.1 5.2 4 57.4 60.3 58.8 2.0 3.5 5 48.7 53.3 51.0 3.3 6.4 -
Nomal Mouse plasma K2EDTA, CD-1(ICR), male No. Measured(mg/dL) mean SD CV(%) 1 28.8 31.7 30.2 2.0 6.7 2 132 145 139 9.5 6.9 3 62.7 68.0 65.4 3.8 5.8 4 29.5 25.8 27.6 2.6 9.5 5 58.8 55.1 56.9 2.7 4.7
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Nomal Canine plasma EDTA,Beagle,male No. Measured(mg/dL) mean SD CV(%) Age(years) 1 37.2 33.9 35.5 2.3 6.5 4 2 29.0 27.6 28.3 1.0 3.6 6 3 32.5 36.0 34.2 2.5 7.4 10 4 33.5 36.4 34.9 2.1 5.9 12 5 33.2 30.3 31.8 2.1 6.6 6 6 24.9 22.9 23.9 1.4 6.0 11 7 31.9 28.4 30.2 2.5 8.2 10 8 63.2 57.0 60.1 4.36 7.3 12 -
Nomal Canine serum,Beagle,male No. Measured(mg/dL) mean SD CV(%) Age(years) 1 35.6 39.7 37.7 2.9 7.7 4 2 33.8 37.0 35.4 2.2 6.3 6 3 28.6 31.0 29.8 1.7 5.7 6 4 102 109 105 4.6 4.4 11 5 37.3 34.2 35.8 2.2 6.0 12 6 25.3 24.4 24.8 0.68 2.7 6 7 18.6 16.5 17.5 1.4 8.1 10 8 47.7 42.7 45.2 3.5 7.9 4
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Nomal Feline plasma EDTA,Short hair,male No. Measured(mg/dL) mean SD CV(%) Age(years) 1 104 104 104 0.21 0.20 0.5 2 34.3 31.8 33.1 1.7 5.3 1.0 3 54.8 49.3 52.1 3.9 7.5 3.0 4 65.1 60.7 62.9 3.1 5.0 0.5 5 43.9 44.4 44.1 0.41 0.94 3.0 6 29.0 31.9 30.5 2.1 6.7 3.0 7 16.8 16.2 16.5 0.4 2.5 0.5 8 25.0 27.2 26.1 1.5 5.8 1.0 -
Nomal Feline serum,Short hair,male No. Measured(mg/dL) mean SD CV(%) Age(years) 1 32.4 35.9 34.1 2.5 7.3 3.0 2 18.4 20.2 19.3 1.3 6.7 3.0 3 33.9 36.6 35.3 1.9 5.4 6.0 4 48.9 53.8 51.3 3.5 6.8 4.0 5 33.9 32.8 33.4 0.82 2.5 6.0 6 45.3 47.1 46.2 1.24 2.7 6.0 7 96.6 88.0 92.3 6.1 6.6 1.0 8 52.3 54.7 53.5 1.7 3.1 1.0
References
- Gao, F. et al. : Evid. Based Complement. Alternat. Med., 2015, 801291 (2015). *Extraction liquid of rat liver
- Funakoshi, T. et al. : Biochem. Biophys. Rep., 13, 39 (2018). *Rat muscle satellite cell
- Moser, V. A. and Pike, C. J. : eNeuro, 4, e0077-17 (2017). *Rat plasma
- Fujii, N. et al. : Aging Cell, 16, 508 (2017). *Rat plasma
- Wang, F. et al. : J. Mol. Endocrinol., 52, 133 (2014). *Rat plasma
- Kato, H. et al. : J. Hepatol., 60, 1032 (2014). *Extraction liquid of mouse liver
- Fujimori, K. et al. : Prostaglandins. Other. lipid. Mediat., 94, 96 (2011). *Extraction liquid of mouse culture cells
Overview / Applications
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For research use or further manufacturing use only. Not for use in diagnostic procedures.
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