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LabAssay ™ Phospholipid

Manufacturer :
FUJIFILM Wako Shibayagi Corporation
Storage Condition :
Keep at 2-10 degrees C.
Molecular Weight :
0
GHS :
  • Structural Formula
  • Label
  • Packing
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Product Number
Package Size
Price
Inventory
Distributor
639-51001
Manufacturer
LABPLIP-M1
500Tests
List Price
265.00 USD

In stock

Document

Package Insert
Spectral Data
Certificate of Analysis
Calibration Certificate

Kit component

Kit component

Buffer 50 mL x 3 vials
Chromogen Substrate for 50 mL x 3 vials
Standard Solution 5 mL x 1 vial

Overview

※For research purposes only with animal serum/plasma, Not for Diagnostic Use.

Introduction

Phospholipids are known as not only as major component of cell membranes but also perform vital functions within the body such as emulsification and absorption of fats or coagulation of blood.

Assay principle

Phospholipids are hydrolyzed by Phospholipase D to release hydrogen peroxide. The formed hydrogen peroxide promotes oxidative condensation of N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethoxyaniline sodium salt(DAOS) with 4-aminoantipyrine. LabAssay™ Phospholipid is a kit to determine Phospholipid concentration in samples by measuring absorbance of a blue color which is generated by the oxidative condensation reaction.

Performance

Sample Serum, plasma (Mouse, Rat)
Standard curve range 75.0 ~ 596.1 mg/dL (mg/100 mL)
Measurement time Approx. 10 min.
Amount of sample 2 μL
Measurement wavelength 600 nm(Main), 700 nm(Sub)

Standard Curve

04093472_img01.png

Procedure

Test Standard Blank
Chromogen reagent 300 μL 300 μL 300 μL
Sample Serum 2 μL Standard solution 2 μL
Mix well and incubate at 37℃ for 5 min. Measure the absorbance at 600nm*2 of the test sample and standard solution with the blank solution as the control.

*2: In two wavelength assay, measure using Main wavelength 600 nm/Sub wavelength 700 nm.

Data

Spike-and-Recovery Test

Spike and Recovery Test of Phospholipid, a known amount of standard is spiked into in mouse and rat sample n=2

Rat Sample Spike(mg/dL) Mean(mg/dL) Recovery(mg/dL) Recovery(%)
Serum 0.0 138.1 -   -
152.4 291.0 152.9 100.3%
204.2 345.1 207.0 101.4%
262.3 395.4 257.3 98.1%
314.2 459.7 321.6 102.3%
Plasma (EDTA) 0.0 97.8  - - 
152.4 246.3 148.5 97.4%
204.2 298.6 200.8 98.3%
262.3 352.3 254.5 97.0%
314.2 422.5 324.7 103.3%
Mouse Sample Spike(mg/dL) Mean(mg/dL) Recovery(mg/dL) Recovery(%)
Serum 0.0 285.3 -   -
152.4 438.1 152.8 100.2%
204.2 486.6 201.3 98.6%
262.3 532.0 246.7 94.0%
314.2 601.3 316.0 100.6%
Plasma (EDTA) 0.0 111.5 -  - 
152.4 256.5 145.0 95.1%
204.2 311.2 199.7 97.8%
262.3 368.5 257.0 98.0%
314.2 432.7 321.1 102.2%

Linearity-of-Dilution Test

  • Rat sample
    04093472_img03.png
  • Mouse sample
    04093472_img04.png

Example of measurement

  • 20% Diluted Normal Mouse serum, CD-1(ICR), male
    No. Measured(mg/dL) Mean (mg/dL) SD CV(%)
    1 242.1 239.8 241.0 1.60 0.7
    2 213.6 214.2 213.9 0.44 0.2
    3 185.5 180.8 183.1 3.30 1.8
    4 201.5 194.7 198.1 4.86 2.5
    5 166.8 175.3 171.0 5.97 3.5
  • Normal Mouse plasma K2EDTA, CD-1(ICR), male
    No. Measured(mg/dL) Mean (mg/dL) SD CV(%)
    1 108.2 114.5 111.4 4.45 4.0
    2 124.1 121.8 122.9 1.61 1.3
    3 107.0 99.7 103.4 5.15 5.0
    4 120.2 109.7 115.0 7.41 6.4
    5 134.4 135.2 134.8 0.58 0.4
  • 10% Diluted Normal Rat serum, Sprague Dawley, male
    No. Measured(mg/dL) Mean (mg/dL) SD CV(%)
    1 137.6 131.5 134.6 4.28 3.2
    2 201.3 197.3 199.3 2.88 1.4
    3 167.3 169.6 168.5 1.62 1.0
    4 159.7 160.2 160.0 0.33 0.2
    5 166.0 161.1 163.5 3.52 2.2
  • Normal Rat plasma K2EDTA, Sprague Dawley, male
    No. Measured(mg/dL) Mean (mg/dL) SD CV(%)
    1 99.0 96.4 97.7 1.84 1.9
    2 126.9 118.7 122.8 5.82 4.7
    3 109.5 106.2 107.9 2.35 2.2
    4 121.3 110.7 116.0 7.50 6.5
    5 129.3 113.9 121.6 10.9 8.9
  • Normal Canine serum, Beagle, male
    No. Measured(mg/dL) Mean
    (mg/dL)
    SD CV(%) Age(years)
    1 365.7 381.0 373.3 7.64 2.0 4
    2 283.8 264.4 274.1 9.74 3.6 6
    3 375.1 334.2 354.6 20.49 5.8 6
    4 287.5 288.0 287.8 0.26 0.1 11
    5 459.9 484.8 472.3 12.47 2.6 12
    6 300.2 306.3 303.3 3.06 1.0 6
    7 271.8 278.4 275.1 3.34 1.2 10
    8 276.4 279.3 277.8 1.46 0.5 4
  • Normal Canine plasma EDTA, Beagle, male
    No. Measured(mg/dL) Mean
    (mg/dL)
    SD CV(%) Age(years)
    1 340.4 316.2 328.3 12.11 3.7 4
    2 321.5 345.1 333.3 11.76 3.5 6
    3 351.1 374.4 362.8 11.69 3.2 10
    4 322.8 327.0 324.9 2.10 0.6 12
    5 337.4 364.0 350.7 13.27 3.8 6
    6 303.3 316.7 310.0 6.72 2.2 11
    7 318.9 321.5 320.2 1.31 0.4 10
    8 310.7 306.0 308.4 2.35 0.8 12
  • Normal Feline serum, Short hair, male
    No. Measured(mg/dL) Mean
    (mg/dL)
    SD CV(%) Age(years)
    1 244.8 246.4 245.6 0.80 0.3 3
    2 193.7 188.3 191.0 2.69 1.4 3
    3 236.3 240.1 238.2 1.89 0.8 6
    4 229.7 229.9 229.8 0.13 0.1 4
    5 226.7 227.4 227.0 0.38 0.2 6
    6 239.8 236.2 238.0 1.80 0.8 6
    7 305.3 299.4 302.3 2.93 1.0 1
    8 159.6 157.6 158.6 0.98 0.6 1
  • Normal Feline plasma EDTA, Short hair, male
    No. Measured(mg/dL) Mean
    (mg/dL)
    SD CV(%) Age(years)
    1 214.5 212.0 213.3 1.24 0.6 0.5
    2 114.9 116.9 115.9 0.98 0.8 1
    3 199.8 194.8 197.3 2.52 1.3 3
    4 180.9 184.6 182.8 1.85 1.0 0.5
    5 195.6 201.5 198.5 2.94 1.5 3
    6 143.7 139.3 141.5 2.21 1.6 3
    7 168.2 166.3 167.2 0.92 0.5 0.5
    8 183.7 178.2 181.0 2.73 1.5 1

References

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