Tissue Clearing
The general protocol for tissue clearing with FUJIFILM Wako products follows the guideline shown below:
SCALEVIEW-S | CUBIC | SeeDB2 | SeeDB | CLARITY | ClearSee™ | |
---|---|---|---|---|---|---|
RI ( Refractive index ) | 1.49 | 1.49 | 1.46 / 1.52 | 1.49 | 1.45 | 1.41 |
Types of tissues | ||||||
Brain: > 8 mm | ✓ | ✓ | ||||
Brain: > 4 mm | ✓ | ✓ | ✓ | |||
Brain: > 2 mm | ✓ | ✓ | ✓ | ✓ | ✓ | |
Other organs | ✓ | ✓ | ||||
Bone | ✓ (optional) |
✓ (optional) |
✓ (optional) |
|||
Fish | ✓ | |||||
Insects | ✓ | |||||
Plants | ✓ | |||||
Organoids / Spheroids | ✓ (SCALEVIEW-S4) |
✓ | ||||
Types of microscpes | ||||||
Confocal microscopy | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ |
Two Photon Excitation Microscopy | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ |
Light Sheet Fluorescence Microscopy | ✓ | ✓ | ✓ | |||
Super-resolution microscopy | ✓ | |||||
Electron microscopy | ✓ | |||||
Types of objectives | ||||||
Silicon oil immersion objectives | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ |
Glycerol immersion objectives | ✓ | ✓ | ||||
Water immersion objectives | ✓ | |||||
Types of imaging | ||||||
Fluorescent proteins | ✓ | ✓ | ✓ | ✓ (only weeks) |
✓ | ✓ |
Immunostaining | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ |
Fluorescent dyes etc | ✓ | ✓ | ✓ (Alexa dyes are bleavhing) |
✓ | ✓ | ✓ |
Lipid Preserved | ✓ | ✓ |