Tissue Clearing
The general protocol for tissue clearing with FUJIFILM Wako products follows the guideline shown below:
| SCALEVIEW-S | CUBIC | SeeDB2 | SeeDB | CLARITY | ClearSee™ | |
|---|---|---|---|---|---|---|
| RI ( Refractive index ) | 1.49 | 1.49 | 1.46 / 1.52 | 1.49 | 1.45 | 1.41 |
| Types of tissues | ||||||
| Brain: > 8 mm | ✓ | ✓ | ||||
| Brain: > 4 mm | ✓ | ✓ | ✓ | |||
| Brain: > 2 mm | ✓ | ✓ | ✓ | ✓ | ✓ | |
| Other organs | ✓ | ✓ | ||||
| Bone | ✓ (optional) |
✓ (optional) |
✓ (optional) |
|||
| Fish | ✓ | |||||
| Insects | ✓ | |||||
| Plants | ✓ | |||||
| Organoids / Spheroids | ✓ (SCALEVIEW-S4) |
✓ | ||||
| Types of microscpes | ||||||
| Confocal microscopy | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ |
| Two Photon Excitation Microscopy | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ |
| Light Sheet Fluorescence Microscopy | ✓ | ✓ | ✓ | |||
| Super-resolution microscopy | ✓ | |||||
| Electron microscopy | ✓ | |||||
| Types of objectives | ||||||
| Silicon oil immersion objectives | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ |
| Glycerol immersion objectives | ✓ | ✓ | ||||
| Water immersion objectives | ✓ | |||||
| Types of imaging | ||||||
| Fluorescent proteins | ✓ | ✓ | ✓ | ✓ (only weeks) |
✓ | ✓ |
| Immunostaining | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ |
| Fluorescent dyes etc | ✓ | ✓ | ✓ (Alexa dyes are bleavhing) |
✓ | ✓ | ✓ |
| Lipid Preserved | ✓ | ✓ | ||||