"CLARITY" as a way of a new organization transparency was announced by PhD.Karl Deisseroth and his colleagues at Stanford University at the journal Nature in March 2013 . Since CLARITY makes it possible for the immune staining using a fluorescent protein and antibody, it is expected as a useful tool for the analysis of brain circuits.
VA-044 is a reagent used in the transparent process of the paper protocol.
Chung,K et al. : Nature., 497, 332（2013）.
A mouse brain after CLARITY process
Imaging of a mouse brain after CLARITY process
|Imaging of a mouse brain after CLARITY process||Imaging of a mouse brain after CLARITY process and using antibody|
|Figure 2. fluorescence observation of Thy1-YFP (H Line) mouse brain after CLARITY process
(A) 3-D observation image from the brain cortex to the hippocampus
(B) dendrites image of the pyramidal cell of the cerebral cortex layer V
|Figure 3. fluorescence observation of Thy1-YFP (H Line) mouse brain which has been stained on Iba1 antibody after CLARITY processing
(A) 3-D observation image of microglia cells in the brain cortex
(B) an image of observing cortex Ⅰlayer from the surface layer
|Photographing condition: two-photon
Equipment: Olympus FV1000 upright microscope, a 25-fold immersion objective lens
|Shooting conditions: 1 photon
equipment: Olympus FV1000 upright microscope, a 25-fold immersion objective lens
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