CLARITY
"CLARITY" as a way of a new organization transparency was announced by PhD.Karl Deisseroth and his colleagues at Stanford University at the journal Nature in March 2013 . Since CLARITY makes it possible for the immune staining using a fluorescent protein and antibody, it is expected as a useful tool for the analysis of brain circuits.
VA-044 is a reagent used in the transparent process of the paper protocol.
Reference
Chung,K et al. : Nature., 497, 332（2013）.
Process
A mouse brain after CLARITY process
Imaging
Imaging of a mouse brain after CLARITY process
| Imaging of a mouse brain after CLARITY process | Imaging of a mouse brain after CLARITY process and using antibody |
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| Figure 2. fluorescence observation of Thy1-YFP (H Line) mouse brain after CLARITY process (A) 3-D observation image from the brain cortex to the hippocampus (B) dendrites image of the pyramidal cell of the cerebral cortex layer V |
Figure 3. fluorescence observation of Thy1-YFP (H Line) mouse brain which has been stained on Iba1 antibody after CLARITY processing (A) 3-D observation image of microglia cells in the brain cortex (B) an image of observing cortex Ⅰlayer from the surface layer |
| Photographing condition: two-photon Equipment: Olympus FV1000 upright microscope, a 25-fold immersion objective lens |
Shooting conditions: 1 photon equipment: Olympus FV1000 upright microscope, a 25-fold immersion objective lens |
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CLARITY related products
Electrophoresis tank
For research use or further manufacturing use only. Not for use in diagnostic procedures.
Product content may differ from the actual image due to minor specification changes etc.
If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.