Wako Blog

We will introduce a wide range of topics of research, technical reports, tips on experiments and so on.

Talking of LAL

Talking of LAL

Limulus Amebocyte Lysate (LAL) and Silkworm Larvae Plasma (SLP) Reagents

This article was written by Dr. Masakazu Tsuchiya, FUJIFILM Wako Pure Chemical Corporation, for Vol. 63, No. 1 (January 1995) of Wako Junyaku Jiho.
The content is from the time of the presentation. It is not the latest information due to new knowledge and changes in regulatory rules after original publication.

Limulus amebocyte lysate (LAL) reagent reacts with endotoxin and (1→3)-β-D-glucan (β-glucan), which make up the cell walls of gram-negative bacteria and fungi. Our research has focused on the detectio...

Talking of LAL

Endotoxin-specific LAL

This article was written by Dr. Masakazu Tsuchiya, FUJIFILM Wako Pure Chemical Corporation, for Vol. 62, No. 4 (October 1994) of Wako Junyaku Jiho.
The content of this article is from the time of publication. It is not the latest information due to new knowledge and changes in regulatory rules after original publication.

We have already described how limulus amebocyte lysate (LAL) reacts to both endotoxins and (1→3)-β-D-glucan. When β-glucan is present during an assay, endotoxin sometimes cannot be accurately measured...

Talking of LAL

Detecting Labware Contamination

This article was written by Dr. Masakazu Tsuchiya, FUJIFILM Wako Pure Chemical Corporation, for Vol. 62 No. 3 (July 1994) of Wako Junyaku Jiho.
The content of this article is from the time of publication. It is not the latest information due to new knowledge and changes in regulatory rules after original publication.

When using plastic labware for Limulus Amebocyte Lysate (LAL) tests, special attention should be given to contamination by endotoxins or other LAL activating substances, as well as, adsorption of endo...

Talking of LAL

Plastic Labware

This article was written by Dr. Masakazu Tsuchiya, FUJIFILM Wako Pure Chemical Corporation, for Vol. 64, No. 4 (July 1994) of Wako Junyaku Jiho.
The content of this article is from the time of publication. It is not the latest information due to new knowledge and changes in regulatory rules after original publication.

For the Limulus test, attention should be paid not only to the Limulus reagent (LAL), but also to other elements such as the water used for the bacterial endotoxin test. In this episode we would like ...

Talking of LAL

Water for Bacterial Endotoxin Test

This article was written by Dr. Masakazu Tsuchiya, FUJIFILM Wako Pure Chemical Corporation, for Vol. 62, No. 1 (July 1994) of Wako Junyaku Jiho.
The content of this article is from the time of publication. It is not the latest information due to new knowledge and changes in regulatory rules after original publication.

The Limulus Amebocyte Lysate (LAL) test is a technique for detecting endotoxins that uses a reagent formulated from the blood cells of the Limulus horseshoe crab. Water for the Bacterial Endotoxin Tes...

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