Trichostatin A

Cell Biological Research
For Research on Cell Cycle and Transcription
Histone Deacetylase (HDAC) InhibitorsHistone deacetylase (HDAC) plays a central role in chromatin structure formation associated with the nuclear distribution of DNA. There are presently 17 known types of this enzyme in mammals, which are classifiedinto 3 classes (see table below). Also, HDAC Class III has been reported to be associated with regulation of aging and life span.

• Mammalian HDAC Class I : Localized in the nucleus. Is similar to RPD3 enzyme. [Types : HDAC1-3, 8]
• Mammalian HDAC Class II : Localized in the nucleus and cytoplasm and shuttles between the two. Similar to HDA1 enzyme. [Types: HDAC4-7, 9, 10]
• Mammalian HDAC Class III : NAD-dependent enzyme. Localized in the nucleus and cytoplasm. Also called Sirtuin and is similar to SIR2 enzyme. [Types : Sirt 1-7]

HDAC inhibitors show connections with cell division cycles and differentiation, as well as with antitumor activity and apoptosis-inducing activity through the inhibition of the deacetylating activity of HDAC. They can be used for studies on cellular functions involving histone deacetylase.

[Product List]M344 (Wako Cat. #139-14671 (1 mg)) Inhibitor of HDAC Class I and II. Structure homologue of Trichostain A.ITSA1 (Wako Cat.#093-05251 (5 mg)) Inhibitor of Trichostatin A.MC 1293 (Wako Cat.#136-14681 (5 mg)) Inhibitor of HDAC1 and maize deacetylase.MS-275 (Wako Cat.#132-14661 (1 mg)) Inhibits HDAC1 and HDAC3 but does not affect HDAC8.Piceatannol (Wako Cat.#169-21661 (10 mg)) Activator of HDAC Class III and inhibitor of kinase Syk.Sirtinol (Wako Cat.#197-13671 (5 mg)) Inhibitor of HDAC Class III. Cell-permeable.Splitomicin (Wako Cat.#190-13661 1 mg)) Inhibitor of HDAC Class III. Cell-permeable.Trichostatin A (Wako Cat.#200-11993 (1 mg); #204-11991 (5 mg)) Potent and reversible inhibitor of HDAC Class I and II.Valproic Acid (Wako Cat.#227-01071 (5 g); #225-01072 (25 g)) Inhibitor of HDAC1.

Trichostatin A (TSA), a Streptomyces product, specifically inhibits the cell cycle of normal rat fibroblasts in the G1 and G2 phases at very low concentrations as reported by Yoshida, et al.TSA-induced G2-arrest induces the formation of proliferative tetraploid cells.In addition, nanomolar concentration of TSA have been shown to cause an accumulation of highly acetylated histones in vivo, and markedly inhibit the activity of partially purified histone deacetylase in vitro.TSA appears to be a useful product for researching the multiple functions of histone acetylation in regulatory mechanisms of eukaryotic cell proliferation and differentiation.

Solubility: Soluble in methanol (1mg/10mL), ethanol, acetone, and water.

[Related Products]

• Piceatannol (Wako Cat.#169-21661 (10 mg)) Activator of HDAC Class III and inhibitor of kinase Syk.


• Sirtinol (Wako Cat.#197-13671 (5 mg)) Inhibitor of HDAC Class III. Cell-permeable.


• Trichostatin A (Wako Cat.#203-17561 (1 mg); #209-17563 (5 mg)) Potent and reversible inhibitor of HDAC Class I and II.


• Valproic Acid (Wako Cat.#227-01071 (5 g); #225-01072 (25 g)) Inhibitor of HDAC1.