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ScreenFect™UP-293

for Genetic Research
Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at 2-10 degrees C.
GHS :
  • Structural Formula
  • Label
  • Packing
SDS
Comparison
Product Number
Package Size
Price
Inventory
Distributor
290-80203
Barcode No
4548995068354
1L, for
List Price
693.00 USD

In stock in Japan

Distributor
294-80201
Barcode No
4548995068347
100mL, for
List Price
83.00 USD

In stock in Japan

Document

SDS
Product Specification Sheet
Spectral Data
Certificate of Analysis
Calibration Certificate

Kit component

Kit components for 100mL (294-80201)

ScreenFect™ UP-293 Transfection Reagent 200µL
ScreenFect™ UP-293 Dilution Buffer 6.7mL
ScreenFect™ UP-293 Booste 500µL

Kit components for 1L (290-80203)

ScreenFect™ UP-293 Transfection Reagent 2 x 1 mL
ScreenFect™ UP-293 Dilution Buffer 67 mL
ScreenFect™ UP-293 Booste 5 mL

Materials required

Expression Systems Expi293™ Expression System FreeStyle™ 293 Expression System
Cells Expi293F™ Cells FreeStyle™ 293-F Cells
Medium Expi293F™ Expression Medium FreeStyle™ 293 Expression Medium
Buffer Not require* Opti-MEM™ I Reduced Serum Medium

*Use ScreenFect™ UP-293 Dilution Buffer provided with this kit.

Overview

ScreenFect™ UP-293 is a transfection reagent kit which is highly efficient for production of antibodies and proteins in Expi293F™ or FreeStyle™ 293-F cells. ScreenFect™ UP-293 Booster, which is added to transfected cells, enhances remarkably protein expression level in cells.

  • Low-priced
  • High yield protein expression
  • Just change your transfection reagent

Applications

Comparison of luciferase expression level in Expi293F™ Cells

This figure compares transfection reagent performance in Expi293F™ cells. Cells were transfected with a secreted Luc expression vector using commercially available transfection reagents and cultured in 30 mL of Expi293™ Expression Medium. 96 hours after transfection, cell supernatants were measured for emission intensity by Luciferase assay.

03476960_img01.png

ScreenFect™ UP-293 achieves the highest luciferase expression level in comparison to other commercially available transfection reagents.

Expi293™, Freestyle™, Expi293F™ are registered trademarks of Thermo Fisher Scientific.

High production yield for IgG in Expi293F™ Cells

Heavy and light chains expression vectors were transfected using commercially available transfection reagent in Expi293F™ Cells. The Expi293F™ Cells were cultured in 30 mL of Expi293™ Expression Medium. 120 hours after transfection, supernatants were collected and electrophoresed. IgG was measured by CBB staining.

03476960_img02.png

ScreenFect™ UP-293 has the same capabilities at other company's product.

Comparison of luciferase expression level in FreeStyle™ 293-F Cells

This figure compares transfection reagent performance in FreeStyle™ 293-F Cells. Cells were transfected with a secreted Luc expression vector using commercially available transfection reagent and cultured in 30 mL of FreeStyle™ 293 Expression Medium. Emission intensity of cell supernatants was measured by Luciferase assay, 96 hours post-transfection.

03476960_img03.png

ScreenFect™ UP-293 provides considerably higher expression level than Product C.

Expi293™, Freestyle™, Expi293F™ are registered trademarks of Thermo Fisher Scientific.

Transfection protocol for 30 mL culture volume

1
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One day prior to transfection, prepare culture cells to be appropriate cell numbers for transfection using a 125 mL or 250 mL of Erlenmeyer shake flask.
Note: Recommended seeding cell density is > 0.8 x 106 viable cells/mL.
2
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[Prepare cell suspension]
Just before transfection, prepare 28 mL of cell suspension with the cell densities below in a 125 mL of Erlenmeyer shake flask.
●For Expi293F™ Cells
2.7 x 106 viable cells/mL medium (7.5 x 107 viable cells/28 mL of medium)
●For FreeStyle™ 293-F Cells
1.1 x 106 viable cells/mL medium (3.0 x 107 viable cells/28 mL of medium)
Note: cell viability must not be < 90 %.
3
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Mix ScreenFect™ UP-293 Transfection Reagent well with a vortex mixer.
4
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[Prepare a diluted transfection reagent]
●For Expi293F™ Cells
Add 60 µL of ScreenFect™ UP-293 Transfection Reagent into 940 µL of ScreenFect™ UP-293 Dilution Buffer in a sterile microfuge tube and mix it using pipette strokes.
●For FreeStyle™ 293-F Cells
Add 30 µL of ScreenFect™ UP-293 Transfection Reagent into 970 µL of Opti-MEM™ I Reduced Serum Medium in a sterile microfuge tube and mix it using pipette strokes.
5
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[Prepare a diluted DNA solution]
●For Expi293F™ Cells
Add 30 µg of plasmid DNA with a density of 1µg/µL into 970µL of ScreenFect™ UP-293 Dilution Buffer in a sterile microfuge tube and mix it using pipette strokes.
●For FreeStyle™ 293-F Cells
Add 30 µg of plasmid DNA with a density of 1µg/µL into 970µL of Opti-MEM™ I Reduced Serum Medium in a sterile microfuge tube and mix it using pipette strokes.
6
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[Prepare a lipoplex solution]
Mix the diluted transfection reagent and the diluted DNA solution with rapid pipette strokes and spin down it. Incubate it for 5-20 minutes at room temperature.
Note: Do not vortex the mixed solution.
7
03476960_img10.png
Add the 2 mL of the lipoplex solution into the cell suspension in the Erlenmeyer Shaker Flask with slowly swirling.
Note: The final volume should be approximately 30mL in the flask.
8
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Incubate the cells at 37°C (8% CO2), rotating at 125 rpm on an orbital shaker for 16 hours.
9
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Add 150 µL of ScreenFect™ UP-293 Booster to the cells.
10
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Continue to incubate the cells at 37°C (8% CO2), rotating at 125 rpm on an orbital shaker.
Collect cells or culture supernatants containing target proteins in 48 - 120 hours after transfection.
Note: The incubation period required for successful transfection depends on the target protein and cell viability.

Expi293F™, Freestyle™ and Opti-MEM™ are registered trademarks of Thermo Fisher Scientific.

References

  1. Diefenbacher, Markus E., et al. "The LIM Domain Protein nTRIP6 Recruits the Mediator Complex to AP-1-Regulated Promoters." PLoS ONE 9.5 (2014): e97549.
  2. Freise, Christian, and Uwe Querfeld. "Inhibition of vascular calcification by block of intermediate conductance calcium-activated potassium channels with TRAM-34." Pharmacological Research (2014).
  3. Hagiwara, Akane, et al. "Luteinizing Hormone-Induced Expression of Ptger4b, a Prostaglandin E2 Receptor Indispensable for Ovulation of the Medaka Oryzias latipes, Is Regulated by a Genomic Mechanism Involving Nuclear Progestin Receptor."
  4. Peng, Yanyan, Ruidan Xu, and Xiaofeng Zheng. "HSCARG Negatively Regulates the Cellular Antiviral RIG-I Like Receptor Signaling Pathway by Inhibiting TRAF3 Ubiquitination via Recruiting OTUB1." PLoS pathogens 10.4 (2014): e1004041. (3)
  5. Wakimoto, Hiroaki, et al. "Targetable signaling pathway mutations are associated with malignant phenotype in IDH-mutant gliomas." Clinical Cancer Research (2014). (2)
  6. Fischer, Simon, et al. "Breaking limitations of complex culture media: Functional non-viral miRNA delivery into pharmaceutical production cell lines." Journal of biotechnology 168.4 (2013): 589-600.
  7. Bai, Dongmei, et al. "Regulation of the HDM2-p53 pathway by ribosomal protein L6 in response to ribosomal stress." Nucleic acids research 42.3 (2014): 1799-1811.
  8. Liu, Xing, et al. "Isocitrate dehydrogenase 2 mutation is a frequent event in osteosarcoma detected by a multi‐specific monoclonal antibody MsMab‐1." Cancer medicine 2.6 (2013): 803-814.

Overview / Applications

Outline This product is for research use only. Do not administer it to human.
This is transfection reagent for Expi 293™ Expression System and FreeStyle™ 293 Expression System. It is suitable for large-scale expression of recombinant antibodies and proteins. ScreenFect™ UP-293 Booster added after transfection significantly enhances cellular protein expression.
This product contains transfection reagent only. Cells and media are not included.
[Features]
·Low price
·High expression of putative protein
·Only to change the transfection reagent

[Product composition]
·ScreenFect™ UP-293 Transfection Reagent
·ScreenFect™ UP-293 Dilution Buffer
·ScreenFect™ UP-293 Booster

Expi 293™ and Freestyle™ are registered trademarks of Thermo Fisher Scientific

Property

Manufacturer Information

Alias

Product content may differ from the actual image due to minor specification changes etc.

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