ScreenFect(TM)mRNA
- for Genetic Research
- Manufacturer :
- FUJIFILM Wako Pure Chemical Corporation
- Storage Condition :
- Keep at 2-10 degrees C.
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0.2mL
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In stock in Japan |
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1mL
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In stock in Japan |
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1mL x5
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Discontinued
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※Check availability in the US with the distributor.
Document
Overview
ScreenFect™mRNA is a transfection reagent consisting of a new cationic liposome by click chemistry screening system*1. ScreenFect™mRNA is suitable for transfection into eukaryotic cells (HeLa, HEK293, MDF cells, etc.) without the replacement of culture medium after transfection because it is not included hazard compounds. ScreenFect™mRNA is used for mRNA transfection procedures.
- Direct transfection of mRNA
- Long RNA transfection
- Establish iPS cell line without virus vector
*1: Biomaterials, Nov.; 33(32): 8160-76, 2012.
Outline of ScreenFect™mRNA protocol
The ideal mixing ratio of mRNA and transfection reagent varies depending on the type of cells.
We will recommend you to examine several ratios and choose the best one.
Applications
GFP mRNA transfection into HEK293 cells
| Transfection conditions of ScreenFect™mRNA | |
|---|---|
| Cell Line | HEK293 cells |
| Protocol | 1-Step method, no-medium replacement |
| Well Format | 96 well plate |
| Amount of Transfection Reagent | 0.3 μL/well |
| Amount of RNA | GFP mRNA [TriLink #L-6101] 75 ng/well |
| Detection Time | 24 hours after the transfection |
| Detector | Cytation3 microscope [BioTek] |
EGFP mRNA transfection into HeLa cell (with SFA P-reagent)
| Transfection conditions of ScreenFect™mRNA | |
|---|---|
| Cell Number | 0.7 x 105 cells/well |
| Amount of mRNA | 0.1 μg/assay |
| Ratio | mRNA(μg): ScreenFect™ mRNA reagent (μL) = 1:4 |
| Amount of SFA P-reagent | 0.2 μL/well mRNA (μg): SFA P-reagent (μL) = 1:2 |
| Well Format | 24 well plates |
| Detection Time | 48 hours after the transfection |
ScreenFect™ mRNA transfection conditions
| Plate size | Surface area | Medium volume | Total volume mRNA-lipid complex | mRNA / Dilution Buffer | Transfection Reagent / Dilution Buffer |
|---|---|---|---|---|---|
| 96 wells | 0.3 cm2 | 100 μL | 10 μL | 50 ng / 5 μL | 0.15 or 0.2 μL / 5 μL |
| 24 wells | 2 cm2 | 500 μL | 50 μL | 250 ng / 25 μL | 0.75 or 1.0 μL / 25 μL |
| 12 wells | 4 cm2 | 1,000 μL | 100 μL | 500 ng / 50 μL | 1.5 or 2.0 μL / 50 μL |
| 6 wells | 10 cm2</td. | 2,000 μL | 250 μL | 1,250 ng / 125 μL | 3.75 or 5.0 μL / 125 μL |
References
- Diefenbacher, Markus E., et al. "The LIM Domain Protein nTRIP6 Recruits the Mediator Complex to AP-1-Regulated Promoters." PLoS ONE 9.5 (2014): e97549.
- Freise, Christian, and Uwe Querfeld. "Inhibition of vascular calcification by block of intermediate conductance calcium-activated potassium channels with TRAM-34." Pharmacological Research (2014).
- Hagiwara, Akane, et al. "Luteinizing Hormone-Induced Expression of Ptger4b, a Prostaglandin E2 Receptor Indispensable for Ovulation of the Medaka Oryzias latipes, Is Regulated by a Genomic Mechanism Involving Nuclear Progestin Receptor."
- Peng, Yanyan, Ruidan Xu, and Xiaofeng Zheng. "HSCARG Negatively Regulates the Cellular Antiviral RIG-I Like Receptor Signaling Pathway by Inhibiting TRAF3 Ubiquitination via Recruiting OTUB1." PLoS pathogens 10.4 (2014): e1004041. (3)
- Wakimoto, Hiroaki, et al. "Targetable signaling pathway mutations are associated with malignant phenotype in IDH-mutant gliomas." Clinical Cancer Research (2014). (2)
- Fischer, Simon, et al. "Breaking limitations of complex culture media: Functional non-viral miRNA delivery into pharmaceutical production cell lines." Journal of biotechnology 168.4 (2013): 589-600.
- Bai, Dongmei, et al. "Regulation of the HDM2-p53 pathway by ribosomal protein L6 in response to ribosomal stress." Nucleic acids research 42.3 (2014): 1799-1811.
- Liu, Xing, et al. "Isocitrate dehydrogenase 2 mutation is a frequent event in osteosarcoma detected by a multi‐specific monoclonal antibody MsMab‐1." Cancer medicine 2.6 (2013): 803-814.
Overview / Applications
| Outline | This product is for research use only. Do not administer it to human. ScreenFect™ mRNA is tranfection reagent which includes novel cationic liposome derived from click cemistry screening system. Through screening, this product adopts the most appropriate liposome for mRNA transfection. |
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Manufacturer Information
Alias
- ScreenFect mRNA
ScreenFect messenger RNA
mRNA Transfection
ScreenFect mRNA
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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