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ScreenFect™mRNA

for Genetic Research
Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at 2-10 degrees C.
  • Structural Formula
  • Label
  • Packing
SDS
Comparison
Product Number
Package Size
Price
Inventory
Distributor
293-75901
Barcode No
4548995040596
0.2mL
List Price
132.00 USD

In stock in Japan

Distributor
299-75903
Barcode No
4548995040602
1mL
List Price
517.00 USD

In stock in Japan

Distributor
297-75904
Barcode No
4548995040619
1mL x5
Discontinued

Document

SDS
Product Specification Sheet
Spectral Data
Certificate of Analysis
Calibration Certificate

Overview

ScreenFect™mRNA is a transfection reagent consisting of a new cationic liposome by click chemistry screening system*1. ScreenFect™mRNA is suitable for transfection into eukaryotic cells (HeLa, HEK293, MDF cells, etc.) without the replacement of culture medium after transfection because it is not included hazard compounds. ScreenFect™mRNA is used for mRNA transfection procedures.

  • Direct transfection of mRNA
  • Long RNA transfection
  • Establish iPS cell line without virus vector

*1: Biomaterials, Nov.; 33(32): 8160-76, 2012.

Outline of ScreenFect™mRNA protocol

02124206_img02.gif

The ideal mixing ratio of mRNA and transfection reagent varies depending on the type of cells.
We will recommend you to examine several ratios and choose the best one.

Applications

GFP mRNA transfection into HEK293 cells

02124206_img01.gif
Transfection conditions of ScreenFect™mRNA
Cell Line HEK293
Protocol 1-Step method, no-medium replacement
Well Format 96 well plate
Amount of Transfection Reagent 0.3 μL/well
Amount of RNA GFP mRNA [TriLink #L-6101] 75 ng/well
Detection Time 24 hours after the transfection
Detector Cytation3 microscope [BioTek]

EGFP mRNA transfection into HeLa cell (with SFA P-reagent)

02124206_img03.gif
Transfection conditions of ScreenFect™mRNA
Cell Number 0.7 x 105 cells/well
Amount of mRNA 0.1 μg/assay
Ratio mRNA(μg): ScreenFect™ mRNA reagent(μL) = 1:4
Amount of SFA P-reagent 0.2 μL/well
mRNA(μg): SFA P-reagent(μL) = 1:2
Well Format 24 well plates
Detection Time 48 hours after the transfection

ScreenFect™ mRNA transfection conditions

mRNA transfection (/well) Standard Protocol
Plate size Surface area Medium volume Total volume mRNA-lipid complex Rate of mRNA: Transfection Reagent mRNA / Dilution Buffer Transfection Reagent / Dilution Buffer
96 wells 0.3 cm2 100 μL 10 μL 1:3 50ng / 5 μL 0.15 μL / 5 μL
1:4 0.2 μL / 5 μL
24 wells 2 cm2 500 μL 50 μL 1:3 250ng / 25 μL 0.75 μL / 25 μL
1:4 1.0 μL / 25 μL
12 wells 4 cm2 1000 μL 100 μL 1:3 500ng / 50 μL 1.5 μL / 50 μL
1:4 2.0 μL / 50 μL
6 wells 10 cm2 2000 μL 250 μL 1:3 1250ng / 125 μL 3.75 μL / 125 μL
1:4 5.0 μL / 125 μL

References

  1. Diefenbacher, Markus E., et al. "The LIM Domain Protein nTRIP6 Recruits the Mediator Complex to AP-1-Regulated Promoters." PLoS ONE 9.5 (2014): e97549.
  2. Freise, Christian, and Uwe Querfeld. "Inhibition of vascular calcification by block of intermediate conductance calcium-activated potassium channels with TRAM-34." Pharmacological Research (2014).
  3. Hagiwara, Akane, et al. "Luteinizing Hormone-Induced Expression of Ptger4b, a Prostaglandin E2 Receptor Indispensable for Ovulation of the Medaka Oryzias latipes, Is Regulated by a Genomic Mechanism Involving Nuclear Progestin Receptor."
  4. Peng, Yanyan, Ruidan Xu, and Xiaofeng Zheng. "HSCARG Negatively Regulates the Cellular Antiviral RIG-I Like Receptor Signaling Pathway by Inhibiting TRAF3 Ubiquitination via Recruiting OTUB1." PLoS pathogens 10.4 (2014): e1004041. (3)
  5. Wakimoto, Hiroaki, et al. "Targetable signaling pathway mutations are associated with malignant phenotype in IDH-mutant gliomas." Clinical Cancer Research (2014). (2)
  6. Fischer, Simon, et al. "Breaking limitations of complex culture media: Functional non-viral miRNA delivery into pharmaceutical production cell lines." Journal of biotechnology 168.4 (2013): 589-600.
  7. Bai, Dongmei, et al. "Regulation of the HDM2-p53 pathway by ribosomal protein L6 in response to ribosomal stress." Nucleic acids research 42.3 (2014): 1799-1811.
  8. Liu, Xing, et al. "Isocitrate dehydrogenase 2 mutation is a frequent event in osteosarcoma detected by a multi‐specific monoclonal antibody MsMab‐1." Cancer medicine 2.6 (2013): 803-814.

Overview / Applications

Outline This product is for research use only. Do not administer it to human.
ScreenFect™ mRNA is tranfection reagent which includes novel cationic liposome derived from click cemistry screening system.
Through screening, this product adopts the most appropriate liposome for mRNA transfection.

Property

Manufacturer Information

Alias

  • ScreenFect mRNA
    ScreenFect messenger RNA
    mRNA Transfection
    ScreenFect mRNA

Product content may differ from the actual image due to minor specification changes etc.

If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.

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