ScreenFect™siRNA
- for Genetic Research
- Manufacturer :
- FUJIFILM Wako Pure Chemical Corporation
- Storage Condition :
- Keep at 2-10 degrees C.
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0.2mL
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Out of Stock |
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1mL
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In stock in Japan |
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Document
Overview
ScreenFect™siRNA is a transfection reagent for siRNA consisting of a new cationic liposome screened*1 by click chemistry. ScreenFect™siRNA is compatible with serum and the transfection operation can be carried out simply because of its low cytotoxicity. The constituent reagents contain no poisonous or deleterious substances.
- High knockdown efficiency and low cytotoxicity
- siRNA transfection optimization buffer included
- No need to exchange medium and can be used in the presence of serum
*1: Biomaterials. 2012 Nov; 33(32):8160-6.
Outline of ScreenFect™ siRNA Protocol
The ideal mixing ratio of siRNA and transfection reagent varies depending on the type of cells.
We will recommend you to examine several ratios and choose the best one.
Standard Protocol
| siRNA transfection (/well) Standard Protocol | |||||
|---|---|---|---|---|---|
| Plate size | Surface area | Medium volume | Total volume siRNA-lipid complex | siRNA / Dilution Buffer | ScreenFect™siRNA / Dilution Buffer |
| 96 wells | 0.3 cm2 | 100 μL | 10 μL | 1 pmol / 5 μL | 0.1 μL / 5 μL |
| 24 wells | 2 cm2 | 500 μL | 50 μL | 5 pmol / 25 μL | 0.5 μL / 25 μL |
| 12 wells | 4 cm2 | 1000 μL | 100 μL | 10 pmol / 50 μL | 1.0 μL / 50 μL |
| 6 wells | 10 cm2 | 2000 μL | 250 μL | 25 pmol / 125 μL | 2.5 μL / 125 μL |
Transfection efficiency to cells may vary depending on the type and/or the condition of cells and the combination with the siRNA to be transfected. When starting a transfection experiment, examine several conditions for quantitative ratio and quantities used of siRNA and ScreenFect™siRNA Transfection Reagent, and investigate the optimal conditions.
Refer to the instruction manual included with this product for details on the method of use of ScreenFect™siRNA.
Applications
Examples of use of ScreenFect™ siRNA
Knockdown efficiency of siRNA transfection
siRNA transfection siRNA can be transfected with ScreenFect™ siRNA.
LRP6 siRNA was transfected into MEF cells and the amount of expression of LRP6 was examined by western blotting 24 hours after transfection. ScreenFect™ siRNA showed knockdown efficiency is equal or superior to the competitor's products.
Cytotoxicity
Toxicity in HEK293 cells was verified under fluorescent microscope 24 hours after transfection and ScreenFect™siRNA showed lower cytotoxicity than Company A’s product.
Experiment of tranasfecting PIK3CB siRNA to HeLa cells (adherent cells)
The experiment of transfection of PIK3CB siRNA to HeLa cells was done by both methods of the reverse transfection (1-Step) and the forward transfection (2-Step). The expression quantity of PIK3CB mRNA was measured by real-time quantitative PCR.
Compare the knockdown efficiency from the quantified result against the competitor, ScreenFect™ siRNA showed knockdown efficiency is equal or superior to the competitor’s product.
Comparisons the pereformance of HeLa cells (adherent cells)
Cell Number 1 × 105cells/well Amount of siRNA 5 pmol/assay Amount of Transfection Reagent ScreenFect™siRNA reagent = 0.5 μL
Other company's product = 1.5 μLWell Format 24 well plates Detection Time 48 hours after the transfection
Cell Number 1 × 105cells/well Amount of siRNA 5 pmol/assay Amount of Transfection Reagent ScreenFect™siRNA reagent = 0.5 μL
Other company's product = 1.5 μLWell Format 24 well plates Detection Time 48 hours after the transfection
Overview / Applications
| Outline | This is a transfection reagent comprising a novel cationic liposome identified through click chemistry-based screening. A liposome most suited to siRNA transfection, which has been obtained via screening, is adopted for this product. [Composition] 0.2 mL : ScreenFect™siRNA 0.2 mL, Dilution Buffer for ScreenFect™siRNA 10 mL x 1. 1 mL : ScreenFect™siRNA 1 mL x 1, Dilution Buffer for ScreenFect™siRNA 50 mL x 1. 1 mL x 5 : ScreenFect&trade ; siRNA 1mL x 5, Dilution Buffer for ScreenFect6Trade;siRNA 50mL x 5. |
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Property
Manufacturer Information
Alias
- ScreenFect siRNA
siRNA Transfection
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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