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ScreenFect™siRNA

for Genetic Research
Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at 2-10 degrees C.
  • Structural Formula
  • Label
  • Packing
SDS
Comparison
Product Number
Package Size
Price
Inventory
Distributor
299-75001
Barcode No
4548995034397
0.2mL
List Price
121.00 USD

In stock in Japan

Distributor
295-75003
Barcode No
4548995034403
1mL
List Price
440.00 USD

In stock in Japan

Document

SDS
Product Specification Sheet
Spectral Data
Certificate of Analysis
Calibration Certificate

Overview

ScreenFect™siRNA is a transfection reagent for siRNA consisting of a new cationic liposome screened*1 by click chemistry. ScreenFect™siRNA is compatible with serum and the transfection operation can be carried out simply because of its low cytotoxicity. The constituent reagents contain no poisonous or deleterious substances.

  • High knockdown efficiency and low cytotoxicity
  • siRNA transfection optimization buffer included
  • No need to exchange medium and can be used in the presence of serum

*1: Biomaterials. 2012 Nov; 33(32):8160-6.

Outline of ScreenFect™ siRNA Protocol

01868708_img01.gif

The ideal mixing ratio of siRNA and transfection reagent varies depending on the type of cells.
We will recommend you to examine several ratios and choose the best one.

Standard Protocol

siRNA transfection (/well) Standard Protocol
Plate size Surface area Medium volume Total volume siRNA-lipid complex siRNA / Dilution Buffer ScreenFect™siRNA / Dilution Buffer
96 wells 0.3 cm2 100 μL 10 μL 1 pmol / 5 μL 0.1 μL / 5 μL
24 wells 2 cm2 500 μL 50 μL 5 pmol / 25 μL 0.5 μL / 25 μL
12 wells 4 cm2 1000 μL 100 μL 10 pmol / 50 μL 1.0 μL / 50 μL
6 wells 10 cm2 2000 μL 250 μL 25 pmol / 125 μL 2.5 μL / 125 μL

Transfection efficiency to cells may vary depending on the type and/or the condition of cells and the combination with the siRNA to be transfected. When starting a transfection experiment, examine several conditions for quantitative ratio and quantities used of siRNA and ScreenFect™siRNA Transfection Reagent, and investigate the optimal conditions.
Refer to the instruction manual included with this product for details on the method of use of ScreenFect™siRNA.

Applications

Examples of use of ScreenFect™ siRNA

Knockdown efficiency of siRNA transfection

01868708_img02.jpg

siRNA transfection siRNA can be transfected with ScreenFect™ siRNA.

LRP6 siRNA was transfected into MEF cells and the amount of expression of LRP6 was examined by western blotting 24 hours after transfection. ScreenFect™ siRNA showed knockdown efficiency is equal or superior to the competitor's products.

Cytotoxicity

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Toxicity in HEK293 cells was verified under fluorescent microscope 24 hours after transfection and ScreenFect™siRNA showed lower cytotoxicity than Company A’s product.

Experiment of tranasfecting PIK3CB siRNA to HeLa cells (adherent cells)

The experiment of transfection of PIK3CB siRNA to HeLa cells was done by both methods of the reverse transfection (1-Step) and the forward transfection (2-Step). The expression quantity of PIK3CB mRNA was measured by real-time quantitative PCR.
Compare the knockdown efficiency from the quantified result against the competitor, ScreenFect™ siRNA showed knockdown efficiency is equal or superior to the competitor’s product.

Comparisons the pereformance of HeLa cells (adherent cells)

  • 01868708_img04.jpg

    Cell Number 1 × 105cells/well
    Amount of siRNA 5 pmol/assay
    Amount of Transfection Reagent ScreenFect™siRNA reagent = 0.5 μL
    Other company's product = 1.5 μL
    Well Format 24 well plates
    Detection Time 48 hours after the transfection
  • 01868708_img05.jpg

    Cell Number 1 × 105cells/well
    Amount of siRNA 5 pmol/assay
    Amount of Transfection Reagent ScreenFect™siRNA reagent = 0.5 μL
    Other company's product = 1.5 μL
    Well Format 24 well plates
    Detection Time 48 hours after the transfection

Overview / Applications

Outline This is a transfection reagent comprising a novel cationic liposome identified through click chemistry-based screening. A liposome most suited to siRNA transfection, which has been obtained via screening, is adopted for this product.
[Composition]
0.2 mL : ScreenFect™siRNA 0.2 mL, Dilution Buffer for ScreenFect™siRNA 10 mL x 1.
1 mL : ScreenFect™siRNA 1 mL x 1, Dilution Buffer for ScreenFect™siRNA 50 mL x 1.
1 mL x 5 : ScreenFect&trade ; siRNA 1mL x 5, Dilution Buffer for ScreenFect6Trade;siRNA 50mL x 5.

Property

Manufacturer Information

Alias

  • ScreenFect siRNA
    siRNA Transfection

Product content may differ from the actual image due to minor specification changes etc.

If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.

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