ScreenFect™A
- for Genetic Research
- Manufacturer :
- FUJIFILM Wako Pure Chemical Corporation
- Storage Condition :
- Keep at 2-10 degrees C.
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0.2mL
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In stock in Japan |
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1mL
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In stock in Japan |
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1mL x5
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In stock in Japan |
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Document
Overview
ScreenFect™A is a transfection reagent consisting of a new cationic liposome screened*1 by click chemistry. It can be used with various eukaryote-derived cells and can be added directly to mediums containing antibiotics or serum. DNA and siRNA can be transfected into general experimental cell strains (HeLa, HepG2, MDCK, Cos-7, etc.), stem cells (mouse ES cells, etc.), blood cells (macrophages, THP-1, RAW264, 7, etc.), microglia, primary (initial subculture) cells, and Insect cell. Medium replacement after transfection is not required due to low cytotoxicity. The constituent reagents do not contain any poisonous or deleterious substance.
- High transfection efficiency and low cytotoxicity
- Can be used for both DNA and siRNA
- No need to exchange medium and can be used in the presence of serum
*1 Biomaterials. 2012 Nov; 33(32):8160-6. 2012
Outline of ScreenFect™ A protocol
The ideal mixing ratio of DNA/siRNA and transfection reagent varies depending on the type of cells.
We will recommend you to examine several ratios and choose the best one.
DNA transfection
| DNA transfection (/well) | |||||
|---|---|---|---|---|---|
| Plate size | Surface area | Medium volume | Total volume SF-DNA complex |
DNA / Dilution Buffer |
Transfection Reagent / Dilution Buffer |
| 96 wells | 0.3 cm2 | 100 µL | 10 µL | ～100 ng / 5 µL | 0.25 or 0.3 μL / 5 μL |
| 24 wells | 2 cm2 | 500 µL | 50 µL | ～500 ng / 25 µL | 1.25 or 1.5 μL / 25 μL |
| 12 wells | 4 cm2 | 1,000 µL | 100 µL | ～1,000 ng / 50 µL | 2.5 or 3.0 μL / 50 μL |
| 6 wells | 10 cm2 | 2,000 µL | 250 µL | ～2,500 ng / 125 µL | 6.25 or 7.5 μL / 125 μL |
siRNA transfection
| siRNA transfection (/well) | |||||
|---|---|---|---|---|---|
| Plate size | Surface area | Medium volume | Total volume SF-siRNA complex |
siRNA / Dilution Buffer |
Transfection Reagent / Dilution Buffer |
| 96 wells | 0.3 cm2 | 100 µL | 10 µL | 2～3 pmol / 5 μL | 0.1～0.3 μL / 5 μL |
| 24 wells | 2 cm2 | 500 µL | 50 µL | 10～20 pmol / 25 µL | 0.5～1.5 μL / 25 μL |
| 12 wells | 4 cm2 | 1,000 µL | 100 µL | 20～40 pmol / 50 µL | 1.0～3.0 μL / 50 μL |
| 6 wells | 10 cm2 | 2,000 µL | 250 µL | 20～60 pmol / 125 μL | 3.5～7.5 μL / 125 μL |
Data
Comparison with competitor
GFP-expressing plasmid DNA was transfected into HEK293 cells using ScreenFect™ A. The result demonstrated transfection efficiency is equal or superior to competitors. (96-well plate, GFP-expressing plasmid DNA 75 ng/well)
Transfection efficiency of liposome library
GFP-expressing plasmid DNA was transfected into HEK293T cells using a new cationic liposome library synthesized by click chemistry. As a results, a new liposome (ScreenFect™ A) which can transfect plasmid DNA more efficient than company A was confirmed.
Transfection data
- Low cytotoxicity
GFP-expressing plasmid DNA was transfected into HEK293 cells using ScreenFect™ A. The results demonstrated gene transfection efficiency is equal or superior to competitors. Cytotoxicity was also comparable to competitors. (96-well plate, GFP-expressing plasmid DNA 75 ng/well) - Gene transfection into mouse ES cells
GFP-expressing plasmid DNA was transfected into mouse ES cells using ScreenFect™A and GFP-positive cells were detected. As a result, about 60% or more of mouse ES cells were GFP-positive cells.
About DNA transfection
Also applicable to gene transfection into stem cells.
siRNA transfection
LRP6 siRNA was transfected into HEK293 cells using ScreenFect™ A. It showed that a higher knockdown efficiency than the Company A and the Company B. (96-well plate, final concentration of 1 pmole LRP6 siRNA at 2 nM/well)
GAPDH siRNA was transfected into each cell line using ScreenFect™ A. The results demonstrated that a higher knockdown efficiency than competitor. (96-well plate, final concentration of GAPDH siRNA at 3 nM/well)
siRNA transfection
applicable to siRNA transfection.
More Information
List of Cells transfected by ScreenFect™ A or A plus
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References
- Diefenbacher, Markus E., et al. "The LIM Domain Protein nTRIP6 Recruits the Mediator Complex to AP-1-Regulated Promoters." PLoS ONE 9.5 (2014): e97549.
- Freise, Christian, and Uwe Querfeld. "Inhibition of vascular calcification by block of intermediate conductance calcium-activated potassium channels with TRAM-34." Pharmacological Research (2014).
- Hagiwara, Akane, et al. "Luteinizing Hormone-Induced Expression of Ptger4b, a Prostaglandin E2 Receptor Indispensable for Ovulation of the Medaka Oryzias latipes, Is Regulated by a Genomic Mechanism Involving Nuclear Progestin Receptor."
- Peng, Yanyan, Ruidan Xu, and Xiaofeng Zheng. "HSCARG Negatively Regulates the Cellular Antiviral RIG-I Like Receptor Signaling Pathway by Inhibiting TRAF3 Ubiquitination via Recruiting OTUB1." PLoS pathogens 10.4 (2014): e1004041. (3)
- Wakimoto, Hiroaki, et al. "Targetable signaling pathway mutations are associated with malignant phenotype in IDH-mutant gliomas." Clinical Cancer Research (2014). (2)
- Fischer, Simon, et al. "Breaking limitations of complex culture media: Functional non-viral miRNA delivery into pharmaceutical production cell lines." Journal of biotechnology 168.4 (2013): 589-600.
- Bai, Dongmei, et al. "Regulation of the HDM2-p53 pathway by ribosomal protein L6 in response to ribosomal stress." Nucleic acids research 42.3 (2014): 1799-1811.
- Liu, Xing, et al. "Isocitrate dehydrogenase 2 mutation is a frequent event in osteosarcoma detected by a multi‐specific monoclonal antibody MsMab‐1." Cancer medicine 2.6 (2013): 803-814.
Overview / Applications
| Outline | New Liposome developed by click chemistry DNA & siRNA Transfection Reagent Features:
ScreenFect™ A is a transfection reagent composed of new cationic lipids selected by screening experiments *1 by click chemistry. It is applicable to cell lines from various eukaryotic species and can be directly added to medium containing antibiotic and serum. ScreenFect™ A works with DNA and siRNA and can show highly efficient transfection in common cell lines (HeLa, HepG2, MDCK, Cos-7, etc.), stem cells (mouse embryonic stem cells, etc.), blood cells (macrophage, THP-1, RAW264.7, etc.), microglia, primary culture cells. Because of the low cell toxicity, medium change after transfection is not required. Furthermore, this kit does not contain any poisonous and deleterious components. *1: Biomaterials; 2012 Nov; 33 (32): 8160-6. 2012 Kit Contents:
We are regularly updating the application data of ScreenFect™ A. Please contact us about the applicability of your cell strains. This product is for research use only. Do not administer it to human. |
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Manufacturer Information
Alias
- Transfection
ScreenFect？A
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