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ScreenFect™A

for Genetic Research
Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at 2-10 degrees C.
  • Structural Formula
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Distributor
293-73201
Barcode No
4548995017802
0.2mL
List Price
88.00 USD

In stock in Japan

Distributor
299-73203
Barcode No
4548995017819
1mL
List Price
330.00 USD

In stock in Japan

Distributor
297-73204
Barcode No
4548995017826
1mL x5
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In stock in Japan

Document

SDS
Product Specification Sheet
Spectral Data
Certificate of Analysis
Calibration Certificate

Overview

ScreenFect™A is a transfection reagent consisting of a new cationic liposome screened*1 by click chemistry. It can be used with various eukaryote-derived cells and can be added directly to mediums containing antibiotics or serum. DNA and siRNA can be transfected into general experimental cell strains (HeLa, HepG2, MDCK, Cos-7, etc.), stem cells (mouse ES cells, etc.), blood cells (macrophages, THP-1, RAW264, 7, etc.), microglia, primary (initial subculture) cells, and Insect cell. Medium replacement after transfection is not required due to low cytotoxicity. The constituent reagents do not contain any poisonous or deleterious substance.

  • High transfection efficiency and low cytotoxicity
  • Can be used for both DNA and siRNA
  • No need to exchange medium and can be used in the presence of serum

*1 Biomaterials. 2012 Nov; 33(32):8160-6. 2012

Outline of ScreenFect™ A protocol

01752899_img01.gif

The ideal mixing ratio of DNA/siRNA and transfection reagent varies depending on the type of cells.
We will recommend you to examine several ratios and choose the best one.

DNA transfection

DNA transfection (/well)
Plate size Surface area Medium volume Total volume SF-DNA complex DNA / Dilution Buffer ScreenFect™A / Dilution Buffer
96 wells 0.3 cm2 100 μL 10 μL ~100 ng / 5 μL ~0.6 μL / 5 μL
24 wells 2 cm2 500 μl 50 μL ~500 ng / 25 μL ~3 μL / 25 μL
12 wells 4 cm2 1000 μL 100 μL ~1000 ng / 50 μL ~6 μL / 50 μL
6 wells 10 cm2 2000 μL 250 μL ~2500 ng / 125 μL ~15 μL / 125 μL

siRNA transfection

siRNA transfection (/well)
Plate size Surface area Medium volume Total volume SF-siRNA complex siRNA / Dilution Buffer ScreenFect™A / Dilution Buffer
96 wells 0.3 cm2 100 μL 10 μL ~3 pmol / 5 μL ~0.45 μl / 5 μL
24 wells 2 cm2 500 μL 50 μL ~20 pmol / 25 μL ~2 μl / 25 μL
12 wells 4 cm2 1000 μL 100 μL ~40 pmol / 50 μL ~4 μl / 50 μL
6 wells 10 cm2 2000 μL 250 μL ~90 pmol / 125 μL ~10 μl / 125 μL

Data

Comparison with competitor

01752899_img02.jpg

GFP-expressing plasmid DNA was transfected into HEK293 cells using ScreenFect™ A. The result demonstrated transfection efficiency is equal or superior to competitors. (96-well plate, GFP-expressing plasmid DNA 75 ng/well)

Transfection efficiency of liposome library

01752899_img03.jpg

01752899_img04.jpg

GFP-expressing plasmid DNA was transfected into HEK293T cells using a new cationic liposome library synthesized by click chemistry. As a results, a new liposome (ScreenFect™ A) which can transfect plasmid DNA more efficient than company A was confirmed.

Transfection data

  • Low cytotoxicity
    01752899_img05.jpg

    GFP-expressing plasmid DNA was transfected into HEK293 cells using ScreenFect™ A. The results demonstrated gene transfection efficiency is equal or superior to competitors. Cytotoxicity was also comparable to competitors. (96-well plate, GFP-expressing plasmid DNA 75 ng/well)
  • Gene transfection into mouse ES cells
    01752899_img06.jpg

    GFP-expressing plasmid DNA was transfected into mouse ES cells using ScreenFect™A and GFP-positive cells were detected. As a result, about 60% or more of mouse ES cells were GFP-positive cells.

    About DNA transfection
    Also applicable to gene transfection into stem cells.

siRNA transfection

01752899_img07.jpg

LRP6 siRNA was transfected into HEK293 cells using ScreenFect™ A. It showed that a higher knockdown efficiency than the Company A and the Company B. (96-well plate, final concentration of 1 pmole LRP6 siRNA at 2 nM/well)

01752899_img08.jpg

GAPDH siRNA was transfected into each cell line using ScreenFect™ A. The results demonstrated that a higher knockdown efficiency than competitor. (96-well plate, final concentration of GAPDH siRNA at 3 nM/well)

siRNA transfection
applicable to siRNA transfection.

More Information

List of Cells transfected by ScreenFect™ A or A plus

No. Name of cell
1 143BTK
2 786-O
3 A2058
4 A375
5 A549
6 B16
7 B16F10
8 Ba/F3-CH1
9 BEAS-2B
10 BEL-7402
11 BT549
12 C2C12
13 Cell line from killifish
14 CHO-K1
15 COS-7
16 DB lymphoma
17 DC 2.4
18 Du145
19 EL4
20 Endothelium cell
21 EPC(carp)
22 GP2-293
23 H9C2
24 HCT116
25 HEK293
26 HEK293 TN
27 HEK293A
28 HEK293F
29 HEK293FT
30 HEK293T
31 HeLa
32 HeLa S3
33 HEp-2
34 HepG2
35 hiPSC
36 HK2
37 HKC
38 HL7704
39 HuH-7
40 HUVEC
41 Ins-1
42 L428
No. Name of cell
43 LLC-MK2
44 LO2
45 LoVo
46 MC3T3
47 MC3T3-E1
48 MCF-10
49 MCF-10A
50 MCF-7
51 MDCK
52 MEF
53 mES
54 mHSC
55 Microglia
56 MLEC
57 MS-1
58 Myeloid dendritic cell (MDC)
59 NB1RGB
60 NCI-H1703
61 NE3
62 NIH 3T3
63 NK92
64 OLHNI-2
65 Mouse overy cell
66 PC12
67 Plat-E
68 PLC8024
69 Primary Fibroblast
70 RAW264.7
71 SH-SY5Y
72 SK-Hep1
73 SKOV3
74 T98G
75 TE-13
76 THP-1
77 U-251 MG
78 U2OS
79 U937
80 Vero
81 Drosophira ovary somatic cell
82 HT1080
83 RH7777
84 HaCaT

References

  1. Diefenbacher, Markus E., et al. "The LIM Domain Protein nTRIP6 Recruits the Mediator Complex to AP-1-Regulated Promoters." PLoS ONE 9.5 (2014): e97549.
  2. Freise, Christian, and Uwe Querfeld. "Inhibition of vascular calcification by block of intermediate conductance calcium-activated potassium channels with TRAM-34." Pharmacological Research (2014).
  3. Hagiwara, Akane, et al. "Luteinizing Hormone-Induced Expression of Ptger4b, a Prostaglandin E2 Receptor Indispensable for Ovulation of the Medaka Oryzias latipes, Is Regulated by a Genomic Mechanism Involving Nuclear Progestin Receptor."
  4. Peng, Yanyan, Ruidan Xu, and Xiaofeng Zheng. "HSCARG Negatively Regulates the Cellular Antiviral RIG-I Like Receptor Signaling Pathway by Inhibiting TRAF3 Ubiquitination via Recruiting OTUB1." PLoS pathogens 10.4 (2014): e1004041. (3)
  5. Wakimoto, Hiroaki, et al. "Targetable signaling pathway mutations are associated with malignant phenotype in IDH-mutant gliomas." Clinical Cancer Research (2014). (2)
  6. Fischer, Simon, et al. "Breaking limitations of complex culture media: Functional non-viral miRNA delivery into pharmaceutical production cell lines." Journal of biotechnology 168.4 (2013): 589-600.
  7. Bai, Dongmei, et al. "Regulation of the HDM2-p53 pathway by ribosomal protein L6 in response to ribosomal stress." Nucleic acids research 42.3 (2014): 1799-1811.
  8. Liu, Xing, et al. "Isocitrate dehydrogenase 2 mutation is a frequent event in osteosarcoma detected by a multi‐specific monoclonal antibody MsMab‐1." Cancer medicine 2.6 (2013): 803-814.

Overview / Applications

Outline New Liposome developed by click chemistry
DNA & siRNA Transfection Reagent

Features:

  1. Highly efficient transfection & Low cell toxicity

  2. Applicable to both DNA & siRNA

  3. Requires no medium change after transfection

  4. Serum Compatible

ScreenFect™ A is a transfection reagent composed of new cationic lipids selected by screening experiments *1 by click chemistry. It is applicable to cell lines from various eukaryotic species and can be directly added to medium containing antibiotic and serum.

ScreenFect™ A works with DNA and siRNA and can show highly efficient transfection in common cell lines (HeLa, HepG2, MDCK, Cos-7, etc.), stem cells (mouse embryonic stem cells, etc.), blood cells (macrophage, THP-1, RAW264.7, etc.), microglia, primary culture cells.

Because of the low cell toxicity, medium change after transfection is not required. Furthermore, this kit does not contain any poisonous and deleterious components.

*1: Biomaterials; 2012 Nov; 33 (32): 8160-6. 2012

Kit Contents:

  1. Transfection Reagent

  2. Dilution Buffer

We are regularly updating the application data of ScreenFect™ A. Please contact us about the applicability of your cell strains.

This product is for research use only. Do not administer it to human.
[Note] Not available for sale in China.

Property

Manufacturer Information

Alias

  • Transfection
    ScreenFect?A

Product content may differ from the actual image due to minor specification changes etc.

If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.

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