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Anti 6×Histidine, Monoclonal Antibody(28-75)

for Immunochemistry
Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at 2-10 degrees C.
Host :
Mouse
Clone No. :
28-75
Classification :
Monoclonal
  • Structural Formula
  • Label
  • Packing
SDS
Comparison
Product Number
Package Size
Price
Availability
Certificate of Analysis
Purchase
Distributor
018-23224
Barcode No
4548995013682
1mg
Discontinued
Distributor
014-23221
Barcode No
4987481613492
200ug
List Price
473.00 USD

In stock in Japan

Document

SDS
Product Specification Sheet
Spectral Data
Certificate of Analysis
Calibration Certificate

Application

Overview / Applications

Outline The 6xHistidine polypeptide has been used as the tag for detection and/or purification of recombinant protein.
Monoclonal anti 6xHistidine (6xHis) antibody was produced by hybridoma clone 28-75 fusing mouse myeloma cells P3U1 and lymphocytes of BALB/c mice immunized with synthetic HHHHHH peptides which was coupled with keyhole limpet hemocyanin (KLH).
This antibody was purified from mouse ascites by using Protein A affinity chromatography.
Concentration: Indicated on the label.

Formulation: 50 mmol/L MOPS, 150 mmol/L sodium chloride, 0.1 w/v% sodium azide.

Clone No.: 28-75

Subclass: IgG3, κ

Specificity: Specific for N and C terminal of 6 X His tagged recombinant protein.

Working Dilution:
Western blot 1:500 - 1:4,000
Immunoprecipitation 1-10 μg/20 μL Protein G beads slurry
ELISA 1:1,000 - 1:100,000
This product is for research use only. Do not administer it to human.

Applicable to immunoprecipitation and a best suited antibody for immunoprecipitation.

Preparation This antibody was purified from mouse ascites by using Protein A affinity chromatography.
Instructions Western Blot(1:500-1:2,000), Immunoprecipitation(1-10ug/ml ProteinG Beads), ELISA(1-2ug/mL)

Property

Appearance Liquid
Origin / Source Mouse ascites
Specificity Specific for N and C terminal of 6×His tagged recombinant protein.
Composition 50mmol/l MOPS, 150mmol/l Sodium chloride, 0.1w/v% Sodium azide.
Concentration 1.0mg/ml

Antibody Information

Antigen 6×Histidine
Antigen Alias 6HIS Peptide
6Xhis
6xHis (Hexahistidine Polyhistidine)
6-His
6-His (HHHHHH)
6-His tag
6X-HIS Epitope Tag
(His)6 [His-Tag]
6x-His Tag
6×Histidine
Subclass IgG3・κ

Manufacturer Information

Alias

  • 6×His
    His

For research use or further manufacturing use only. Not for use in diagnostic procedures.

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