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6 × His tag

FUJIFILM Wako supplies antibodies against 6xHis (histidine)-tag can be used to detect 6xHis-tagged fusion proteins. The specificity of the recognition site and application differ depending on the clone.

 

Clone No. Recognition site Western blot Immunoprecipitation ELISA Labelling Product Number
N-terminal C-terminal N-terminal C-terminal
9F2 C-terminal ND +++++ ND ++ - - 010-21861
9C11 N/C-terminals +++++ ++++ +++++ ++ - 011-23091
21-48 N/C-terminals +++ ++++ +++ +++ - 017-23211
28-75 N/C-terminals ++ ++ +++++ +++++ - 014-23221
9F2 C-terminal ND +++++ - - - HRP 013-23171
9C11 N/C-terminals +++++ ++++ - - HRP 010-23181

Performance Comparison

Performance comparison of Western blotting


6xHis-Protein Z: expressed in HEK293T (1 x 105 cells)
Protein P-6xHis: expressed in CHO cells (1 x 105 cells)
Primary Antibody concentration: 1μg/mL
Secondary antibody: anti-mouse IgG antibody, 5,000-fold diluted solution

Detection sensitivity equal or superior to that of Competitive product B was confirmed.

Selection Guide

  • Which clone works best in the detection of N-terminal?

     WB:9C11 、IP: 9C11, 28-75

    Which clone works best in the detection of C-terminal?

     WB:9F2 、IP:28-75

    Which clone can recognize the both of N- and C-terminals and is best suited for Western blotting (WB)?

     9C11

    Which clone can recognize the both of N- and C-terminals and is best suited for immunoprecipitation (IP)?

     28-75

  • Which clone is versatile and can handle both WB and IP?

     21-48

    Which clone works best with WB?

     9C11

    Which clone works best with IP?

     28-75

    Which is the cheapest clone to recognize the C-terminal alone ?

     9F2

Product List

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Anti 6×His tag, Monoclonal Antibodies

Labeled Anti 6×His tag, Monoclonal Antibodies

Anti 6×His tag, Antibody Beads

Ni-NTA Agarose (Resin)

Ni-NTA Agarose (Cartridge)

Ni-NTA Agarose (Resin, Highly Cross-linked Agarose Beads)

6×His Peptides

Product content may differ from the actual image due to minor specification changes etc.

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