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Detail Information
  1. Top
  2. Life Science
  3. Protein Research
  4. Affinity Tags
  5. 6 × His tag
  6. Anti 6×Histidine, Monoclonal Antibody(9F2)

Anti 6×Histidine, Monoclonal Antibody(9F2)

for Immunochemistry
Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at -20 degrees C.
Host :
Mouse
Clone No. :
9F2
Classification :
Monoclonal
GHS :
Citations (6)
PubMed Central® search
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  • Structural Formula
  • Label
  • Packing
SDS
Comparison
Product Number
Package Size
Price
Availability
Certificate of Analysis
Purchase
Distributor
014-21864
Barcode No
4548995013446
1mg
Discontinued
Distributor
Distributor
010-21866
Barcode No
4548995013453
5mg
Discontinued
Distributor
Distributor
010-21861
Barcode No
4987481478015
200ug
List Price
352.00 USD

In stock in Japan
Distributor

※Check  availability  in the US with the distributor.

Document

SDS
Product Specification Sheet
Spectral Data
Certificate of Analysis
Calibration Certificate

Application

Overview / Applications

Outline This product is used for detection of C terminal 6His fusion recombinant protein by using immunoprecipitation and western blot.
This product is for research use only. Do not administer it to human.

The 6xhistidine polypeptide has been used as the tag for detection and/or purification of recombinant protein.
Monoclonal Anti 6xhistidine (6xHis) antibody was produced by hybridoma clone 9F2. This clone was established by fusing mouse myeloma cells and lymphocytes of mice immunized with synthetic 6xHis peptide that was coupled with keyhole limpet hemocyanin (KLH). This antibody was purified from mouse ascites.

[Concentration]
Indicated on the label.

[Formulation]
1xPBS aqueous solution with 50% glycerol.

[Clone No.]
9F2

[Subclass]
IgG1

[Specificity]
Specific for C-terminal of 6xHis tagged recombinant protein.

[Working Concentration]
Western blot 0.2 - 2 µg/mL
Immunoprecipitation 2 - 3 µg/20 µL Protein G beads slurry
Instructions Western Blot(0.5ug/mL), Immunoprecipitation(2.5ug/300mL cell lysis solution).

Property

Appearance Liquid
Composition PBS aqueous solution with 50% glycerol.
Concentration 0.4 - 0.6mg/mL Protein

Antibody Information

Antigen 6×Histidine
Antigen Alias 6HIS Peptide
6Xhis
6xHis (Hexahistidine Polyhistidine)
6-His
6-His (HHHHHH)
6-His tag
6X-HIS Epitope Tag
(His)6 [His-Tag]
6x-His Tag
6×Histidine

Manufacturer Information

Alias

  • Anti 6×His, Monoclonal Antibody

For research use or further manufacturing use only. Not for use in diagnostic procedures.

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