Anti 6×Histidine, Monoclonal Antibody(9F2), Peroxidase Conjugated
- for Immunochemistry
- Manufacturer :
- FUJIFILM Wako Pure Chemical Corporation
- Storage Condition :
- Keep at -20 degrees C.
- Host :
- Mouse
- Clone No. :
- 9F2
- Classification :
- Monoclonal
- GHS :
-
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- Structural Formula
- Label
- Packing
- SDS
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Comparison
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Certificate of Analysis
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100uL
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In stock in Japan |
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500uL
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Discontinued
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※Check availability in the US with the distributor.
Document
Application
Overview / Applications
| Outline | The 6xHistidine polypeptide has been used as the tag for detection and/or purification of recombinant protein. Monoclonal anti 6xHistidine (6xHis) antibody was produced by hybridoma clone 9F2. This clone was established by fusing mouse myeloma cells and spleen cells of mice immunized with synthetic 6xHis peptide that was coupled with keyhole limpet hemocyanin (KLH). This antibody was purified from mouse ascites. This antibody was conjugated with horseradish peroxidase. |
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| Preparation | This antibody was purified from mouse ascites by using Protein A affinity chromatography. |
| Application | Western Blot(1:4,000-1:20,000) |
Property
| Appearance | Liquid |
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| Specificity | Specific for C terminal of 6×His tagged recombinant protein. |
| Concentration | Protein : 1mg/mL |
Antibody Information
| Antigen | 6×Histidine |
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| Antigen Alias | 6HIS Peptide 6Xhis 6xHis (Hexahistidine Polyhistidine) 6-His 6-His (HHHHHH) 6-His tag 6X-HIS Epitope Tag (His)6 [His-Tag] 6x-His Tag 6×Histidine |
| Subclass | IgG1 |
| Labeling | Peroxidase |
Manufacturer Information
Alias
- 6×His
His
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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