Pesticides Mixture Standard Solutions for JP Water Quality Act

• WQ1: 66 Pesticides Mixture Standard Solution WQ-1-2
• WQ2: 15 Pesticides Mixture Standard Solution WQ-2
• WQ3: 28 Pesticides Mixture Standard Solution WQ-3
• WQ4: 63 Pesticides Mixture Standard Solution WQ-4
• WQ5: 48 Pesticides Mixture Standard Solution WQ-5
• WQ6: Pesticides Mixture Standard Solution WQ-6
• WQ7: Pesticides Mixture Standard Solution WQ-7
• WQ8: Pesticides Mixture Standard Solution WQ-8
• WQ9: 29 Pesticides Mixture Standard Solution WQ-9
• WQ10: 3 Pesticides Mixture Standard Solution WQ-10

66 Pesticides Mixture Standard Solution WQ-1-2 (each 20 μg/mL Acetone Solution)

GC/MS Chromatogram*

Peak No.	Constituent	Monitoring Ions (m/z)			Peak No.	Constituent	Monitoring Ions (m/z)			Peak No.	Constituent	Monitoring Ions (m/z)
1	Dichlorvos (DDVP)	79	109	185	23	Alachlor	188	160	146	45	Flutolanil	173	145	281
2	Trichlorfon (DEP) (deg.)	109	75	185	24	Tolclofos-methyl	265	125	250	46	Napropamide	72	128	100
3	Dichlobenil (DBN)	171	100	173	25	Dithiopyr	354	306	286	47	Pretilachlor	176	238	262
4	Etridiazole (Echlomezol)	211	183	213	26	Metalaxyl	160	206	132	48	Isoprothiolane	118	189	290
5	Chloroneb	191	193	206	27	Simetryn	213	170	155	49	Buprofezin	105	175	106
6	Isoprocarb (MIPC)	121	136	122	28	Fenitrothion (MEP)	277	260	125	50	Isoxathion	105	177	313
7	Molinate	126	98	188	29	Malathion (Malathon)	127	173	93	51	Mepronil	119	269	91
8	Fenobucarb (BPMC)	121	208	150	30	Esprocarb	91	222	162	52	Chlornitrofen (CNP)	317	319	289
9	Trifluralin	306	264	290	31	Chlorpyrifos	197	199	314	53	Propiconazole	259	173	261
10	Benfluralin (Bethrodine)	292	264	276	32	Thiobencarb (Benthiocarb)	100	72	125	54	Edifenphos (EDDP)	109	173	310
11	Pencycuron	125	180	127	33	Fenthion (MPP)	278	153	125	55	Thenylchlor	127	288	141
12	Dimethoate	87	125	93	34	Fthalide	243	241	215	56	Pyributicarb	165	108	181
13	Simazine (CAT)	201	186	173	35	Pendimethalin	252	191	281	57	Pyridaphenthion	340	199	125
14	Atrazine	200	215	173	36	Isofenphos	213	121	185	58	Iprodione	314	316	187
15	Diazinon	179	137	304	37	Dimethametryn	212	255	240	59	Piperophos	122	140	320
16	Propyzamide	173	145	175	38	Methyldymron	107	119	91	60	EPN	157	169	185
17	Pyroquilon	173	130	144	39	Phenthoate (PAP)	274	125	93	61	Anilofos	226	125	228
18	Disulfoton (Ethylthiomethon)	89	97	186	40	Procymidone	283	96	285	62	Bifenox	341	310	343
19	Chlorothalonil (TPN)	266	264	268	41	Dimepiperate	119	145	91	63	Pyriproxyfen	136	226	137
20	Iprobenfos (IBP)	204	246		42	Captan	79	149	117	64	Mefenacet	192	120	136
21	Terbucarb (MBPMC)	205	220	206	43	Methidathion (DMTP)	145	85	302	65	Cafenstrole	100	188	167
22	Bromobutide	119	232	120	44	Butamifos	286	258	200	66	Etofenprox	163	135	183

• Analysis conditions, charts, and detected ions are reference data. It is not the data to guarantee the pesticide mixture standard solution.

Analysis conditions

[ Instrument ]

Shimadzu QP-2010 Ultra

[ GC ]

Column： BPX5　0.25 μm, 0.25 mm × 30 m

Column temperature： 50℃ (2 min)→10℃/min→220℃→2.5℃/min→270℃→10℃/min→300℃ (5 min)

Injection temperature： 220℃

Carrier gas： He 2.0 mL/min

Splitless： 2 min

[ MS ]

Ionization： EI

Interface temperature： 250℃

15 Pesticides Mixture Standard Solution WQ-2 (each 20 μg/mL Acetone Solution)

GC/MS Chromatogram*

Peak No.	Constituent	Monitoring Ions (m/z)			Peak No.	Constituent	Monitoring Ions (m/z)
1	Diazinon Oxon	137	273	288	9	Isoxathion Oxon	161	105	125
2	Tolclofos-methyl Oxon	249	109	251	10	MPP Oxon Sulfoxide	262	278	247
3	MEP Oxon	244	109	261	11	MPP Oxon Sulfone	294	109	215
4	Malaoxon	127	195	99	12	MPP Sulfoxide	278	125	294
5	MPP Oxon	262	109	247	13	MPP Sulfone	310	125	231
6	Chlorpyrifos Oxon	270	242	298	14	Benzoepin Sulfate	272	274	229
7	Isofenphos Oxon	229	201	314	15	EPN Oxon	141	169	306
8	Butamifos Oxon	244	216	287

• Analysis conditions, charts, and detected ions are reference data. It is not the data to guarantee the pesticide mixture standard solution.

Analysis conditions

[ Instrument ]

Shimadzu QP-2010 Ultra

[ GC ]

Column： BPX5　0.25 μm, 0.25 mm × 30 m

Column temperature： 80℃ (1 min)→15℃/min→170℃→3 ℃/min→200℃→10℃/min→300℃ (3 min)

Injection temperature： 220℃

Carrier gas： He 1.8 mL/min

Splitless： 1 min

[ MS ]

Ionization： EI

Interface temperature： 280℃

28 Pesticides Mixture Standard Solution WQ-3 (each 20 μg/mL Acetonitrile Solution)

LC/MS Chromatogram*

Peak No.	Constituent	Monitoring Ions (m/z)		Mode	Peak No.	Constituent	Monitoring Ions (m/z)		Mode
		Precursor	Product				Precursor	Product
1	Asulam	231.1	156.0	+	15	Flazasulfuron	408.2	182.0	+
2	Methomyl	163.1	88.0	+	16	Azoxystrobin	404.1	372.0	+
3	MPP Oxon Sulfoxide	279.1	263.9	+	17	Dymron	269.2	151.1	+
4	Tricyclazole	190.1	136.0	+	18	Iprodione	330.2	244.9	+
5	Thiodicarb	355.1	87.8	+	19	Carpropamid	334.1	139.1	+
6	MPP Oxon Sulfone	295.2	216.9	+	20	Fenthion (MPP)	279.2	168.9	+
7	MPP Sulfoxide	295.2	109.0	+	21	Diuron (DCMU)	233.2	71.9	-
8	Carbofuran	222.3	165.0	+	22	2,4-D (2,4-PA)	219.0	161.1	-
9	Thiram (Thiuram)	241.2	87.8	+	23	Triclopyr	253.9	196.1	-
10	Probenazole	224.1	41.1	+	24	Mecoprop (MCPP)	213.2	141.1	-
11	Bentazone	239.2	132.2	+	25	Siduron	277.2	45.1	-
12	MPP Oxon	263.1	230.9	+	26	Halosulfuron-methyl	433.1	252.1	-
13	Bensulfuron-methyl	411.1	149.0	+	27	Fipronil	437.0	332.0	-
14	MPP Sulfone	311.2	125.0	+	28	Bensulide	396.2	213.2	-

• Analysis conditions, charts, and detected ions are reference data. It is not the data to guarantee the pesticide mixture standard solution.

Analysis conditions

[ Instrument ]

Shimadzu LCMS-8040

[ HPLC ]

Column： Wakopak^® Ultra C18-3　2.0 × 150 mm

Column temperature： 40℃

Eluent： A) 0.1% HCOOH in H₂O, B) CH₃CN

Gradient：

Time (min)	B conc. (%)
0-30	0-100
30-35	100
35-35.01	100-0
35.01-45	0

Flow rate： 0.2 mL/min

[ MS ]

Ionization： ESI

Mode： MRM

63 Pesticides Mixture Standard Solution WQ-4 (each 20 μg/mL Acetonitrile Solution)*¹

LC/MS Chromatogram*²

Peak No.	Constituent	Monitoring Ions (m/z)		Mode	Peak No.	Constituent	Monitoring Ions (m/z)		Mode	Peak No.	Constituent	Monitoring Ions (m/z)		Mode
		Precursor	Product				Precursor	Product				Precursor	Product
1	Oxamyl	237	72	+	22	Simeconazole	294	70	+	43	Indanofan	341	175	+
2	Pymetrozine	218	105	+	23	Naproanilide	292	171	+	44	Diflubenzuron	311	158	+
3	Thiamethoxam	292	211	+	24	Tebufenozide	351	133	+	45	Tetrachlorvinphos (CVMP)	367	127	+
4	Trinexapac-ethyl	253	69	+	25	Difenoconazole	406	251	+	46	Tebuconazole	308	70	+
5	Cinosulfuron	414	183	+	26	Triflumizole	346	278	+	47	Cyprodinil	226	108	+
6	Acetamiprid	223	126	+	27	Benzofenap	431	105	+	48	Fentrazamide	350	154	+
7	Thiacloprid	253	126	+	28	Quizalofop-ethyl	373	299	+	49	Etobenzanid	340	179	+
8	Pyrazosulfuron-ethyl	415	182	+	29	Oxaziclomefone	376	190	+	50	Oxadiargyl	358	341	+
9	Cyanazine	241	214	+	30	Dinotefuran	203	129	+	51	Phoxim	299	77	+
10	Bromacil	261	205	+	31	Nitenpyram	271	56	+	52	Pirimiphos-methyl	306	164	+
11	Bendiocarb	224	167	+	32	Monocrotophos	224	193	+	53	Pyrazolate	439	91	+
12	Ethoxysulfuron	399	261	+	33	Imidacloprid	256	209	+	54	Clomeprop	324	120	+
13	Furametpyr	334	157	+	34	Clothianidin	250	132	+	55	Pentoxazone	371	286	+
14	(E)-Metominostrobin	285	196	+	35	Metribuzin	215	49	+	56	Silafluofen	426	287	+
15	(Z)-Pyriminobac-methyl*3	362	330	+	36	Fluazifop	328	282	+	57	MCPA (MCP)	199	141	-
16	Ametryn	228	186	+	37	Linuron	249	182	+	58	Dichlorprop	233	161	-
17	(E)-Pyriminobac-methyl*3	362	330	+	38	Acibenzolar-S-methyl	211	136	+	59	Propanil (DCPA)	216	160	-
18	Cumyluron	303	185	+	39	Boscalid	343	307	+	60	Inabenfide	337	122	-
19	Cyproconazole	292	70	+	40	Benzobicyclon	447	257	+	61	Tiadinil	266	71	-
20	Prometryn	242	158	+	41	Diclomezine	255	141	+	62	Thifluzamide	527	125	-
21	Chromafenozide	395	175	+	42	Tetraconazole	372	159	+	63	Flusulfamide	413	171	-
										64	Fluazinam	463	416	-

*1 This product contains 2% methanol.
*2 Analysis conditions, charts, and detected ions are reference data. It is not the data to guarantee the pesticide mixture standard solution.
*3 The preparation concentration is converted to 20 µg/mL as a total of E and Z isomer (each 10 μg/mL).

Analysis conditions

[ Instrument ]

Shimadzu LCMS-8040

[ HPLC ]

Column： Wakopak^® Ultra C18-3　2.0 × 75 mm

Column temperature： 40℃

Eluent： A) 5 mmol/L CH₃COONH₄ in H₂O, B) 5 mmol/L CH₃COONH₄ in CH₃OH

Gradient：

Time (min)	B conc. (%)
0-2	5
2-6	5-40
6-41	40-75
41-46	75-100
46-50	100
50-50.01	100-5
50.01-60	5

Flow rate： 0.15 mL/min

[ MS ]

Ionization： ESI

Mode： MRM

48 Pesticides Mixture Standard Solution WQ-5 (each 20 μg/mL Acetone Solution)

GC/MS Chromatogram*¹

Peak No.	Constituent	Monitoring Ions (m/z)		Peak No.	Constituent	Monitoring Ions (m/z)		Peak No.	Constituent	Monitoring Ions (m/z)
1	Propoxur (PHC)	110	152	18	Chlorthal-dimethyl (TCTP)	301	299	35	(E)-Pyriminobac-methyl*3	302	256
2	Cadusafos	159	158	19	Tetraconazole	336	338	36	Propargite (BPPS)	173	150
3	Cyanophos (CYAP)	243	109	20	Cyanazine	225	212	37	Tebuconazole	250	125
4	Dichlofenthion (ECP)	279	223	21	Quinoclamine (ACN)	207	172	38	Orysastrobin	116	205
5	Benfuresate	163	256	22	Fosthiazate	195	283	39	Acetamiprid	152	126
6	Chlorpyrifos-methyl (Chlorpyrifosmethyl)	286	288	23	Cyprodinil	224	225	40	Cumyluron	267	120
7	Propanil (DCPA)	161	163	24	Thiamethoxam	212	182	41	Furametpyr	157	298
8	Simeconazole	121	73	25	Triflumizole	278	206	42	Indanofan	174	159
9	Metribuzin	198	144	26	Propaphos	220	304	43	Phosalone	182	367
10	Cinmethylin	105	123	27	Butachlor	176	160	44	Cyhalofop-butyl	256	229
11	Prometryn	241	184	28	Tetrachlorvinphos (CVMP)	329	331	45	Pyraclofos	360	194
12	Ametryn	227	212	29	Paclobutrazol	236	125	46	Etobenzanid	179	149
13	Pirimiphos-methyl	290	276	30	(E)-Metominostrobin	191	196	47	Boscalid	140	342
14	(E)-Dimethylvinphos*2	295	297	31	Thifluzamide	194	449	48	Thiacloprid	126	101
15	Bromacil	205	207	32	Uniconazole P	234	236	49	Difenoconazole	323	265
16	Metolachlor	162	238	33	(Z)-Pyriminobac-methyl*3	302	256	50	Pyrazoxyfen	105	91
17	(Z)-Dimethylvinphos*2	295	297	34	Cyproconazole	222	139

*1 Analysis conditions, charts, and detected ions are reference data. It is not the data to guarantee the pesticide mixture standard solution.
*2 The preparation concentration is converted to 20 µg/mL as a total of E and Z isomer (each 10 μg/mL).
*3 The preparation concentration is converted to 20 µg/mL as a total of E and Z isomer.

Analysis conditions

[ Instrument ]

Shimadzu QP-2010 Ultra

[ GC ]

Column： BPX5　0.25 μm, 0.25 mm × 30 m

Column temperature： 50℃ (1 min)→20℃/min→210℃→10℃/min→290℃ (5min)

Injection temperature： 250℃

Carrier gas： He 1.2mL/min

Splitless： 1 min

[ MS ]

Ionization： EI

Interface temperature： 280℃

Pesticides Mixture Standard Solution WQ-6 (Ferimzone, Pyraclonil, Nereistoxin each 20 μg/mL Methanol Solution)

GC/MS Chromatogram*¹

Peak No.	Constituent	Monitoring Ions (m/z)		Mode	Peak No.	Constituent	Monitoring Ions (m/z)		Mode
		Precursor	Product				Precursor	Product
1	Nereistoxin	150	105	+	3	(E)-Ferimzone*2	255	91	+
2	Pyraclonil	315	169	+	4	(Z)-Ferimzone*2	255	91	+

*1 Analysis conditions, charts, and detected ions are reference data. It is not the data to guarantee the pesticide mixture standard solution.
*2 The preparation concentration is converted to 20 µg/mL as a total of E and Z isomer. Ratios of isomer may change during storage.

Analysis conditions

[ Instrument ]

Shimadzu LCMS-8040

[ HPLC ]

Column： Wakopak^® Wakosil-Ⅱ 3C18HG 2.0 × 150 mm

Column temperature： 40℃

Eluent： A) 5 mmol/L CH₃COONH₄ in H₂O, B) 5 mmol/L CH₃COONH₄ in CH₃OH

Gradient：

Time (min)	B conc. (%)
0-25	50-70
25-30	70

Flow rate： 0.2 mL/min

[ MS ]

Ionization： ESI

Mode： MRM

Pesticides Mixture Standard Solution WQ-7 (Iminoctadine, Diquat, Paraquat each 20 μg/mL Water Solution)*¹

LC/MS Chromatogram*²

Peak No.	Constituent	Monitoring Ions (m/z)		Mode	Peak No.	Constituent	Monitoring Ions (m/z)		Mode	Peak No.	Constituent	Monitoring Ions (m/z)		Mode
		Precursor	Product				Precursor	Product				Precursor	Product
1	Iminoctadine Triacetate, Albesilate	179	69	+	2	Diquat	183	157	+	3	Paraquat	185	170	+

*1 When weighing out this product, use a plastic ware made of polytetrafluoroethylene or polypropylene.
*2 Analysis conditions, charts, and detected ions are reference data. It is not the data to guarantee the pesticide mixture standard solution.

Analysis conditions

[ Instrument ]

Shimadzu LCMS-8040

[ HPLC ]

Column： Wakopak^® Wakosil-Ⅱ 5SIL-AQ 2.0 × 150 mm

Column temperature： 40℃

Eluent： HCOONH₄ Buffer : CH₃CN = 70 : 30

Flow rate： 0.4 mL/min

[ MS ]

Ionization： ESI

Mode： MRM

Pesticides Mixture Standard Solution WQ-8 (Glyphosate, Glufosinate, AMPA each 20 μg/mL Water Solution)*¹

LC/MS Chromatogram*²

Peak No.	Constituent	Monitoring Ions (m/z)		Mode	Peak No.	Constituent	Monitoring Ions (m/z)		Mode	Peak No.	Constituent	Monitoring Ions (m/z)		Mode
		Precursor	Product				Precursor	Product				Precursor	Product
1	Glyphosate	390	168	-	2	Glufosinate	402	180	-	3	AMPA ((Aminomethyl)phosphonic Acid)	332	110	-

*1 When weighing out this product, use a plastic ware made of polytetrafluoroethylene or polypropylene.
*2 Analysis conditions, charts, and detected ions are reference data. It is not the data to guarantee the pesticide mixture standard solution.

Analysis conditions

[ Instrument ]

Shimadzu LCMS-8040

[ HPLC ]

Column： Wakopak^® Ultra C18-5 4.6 × 150 mm

Column temperature： 40℃

Eluent： A) 5 mmol/L CH₃COONH₄ in H₂O, B) CH₃CN

Eluent：

Time (min)	B conc. (%)
0-15	30-100

Flow rate： 0.5 mL/min

[ MS ]

Ionization： ESI

Mode： MRM

29 Pesticides Mixture Standard Solution WQ-9 (each 20 μg/mL Acetonitrile Solution)

LC/MS Chromatogram*¹

Peak No.	Constituent	Monitoring Ions (m/z)		Mode	Peak No.	Constituent	Monitoring Ions (m/z)		Mode	Peak No.	Constituent	Monitoring Ions (m/z)		Mode
		Precursor	Product				Precursor	Product				Precursor	Product
1	Methamidophos	142	94	+	12	(Z)-Dimethylvinphos*3	333	127	+	23	Chlorpyrifosmethyl	324	125	+
2	Acephate	184	143	+	13	(5Z)-Orysastrobin*2	392	205	+	24	Cinmethylin	292	105	+
3	Amitraz Metabolite*2	163	107	+	14	(E)-Dimethylvinphos*3	333	127	+	25	Butachlor	312	238	+
4	Tefuryltrione	460	262	+	15	Metolachlor	284	252	+	26	Tolfenpyrad	384	197	+
5	Quinoclamine (ACN)	208	105	+	16	Uniconazole P	292	70	+	27	Dichlofenthion (ECP)	315	259	+
6	Propoxur (PHC)	210	111	+	17	Fenoxanil	329	302	+	28	Cypermethrin	433	191	+
7	Carbaryl (NAC)	202	145	+	18	Propaphos	305	221	+	29	trans-Permethrin*3	408	183	+
8	Fosthiazate	284	104	+	19	Pyrazoxyfen	403	91	+	30	cis-Permethrin*3	408	183	+
9	Benfuresate	274	18	+	20	Pyraclofos	361	257	+	31	Cyanophos (CYAP)	228	118	-
10	Paclobutrazol	294	70	+	21	Phosalone	368	182	+	32	Ethiprole	395	331	-
11	Orysastrobin*2	392	205	+	22	Cadusafos	271	159	+

*1 Analysis conditions, charts, and detected ions are reference data. It is not the data to guarantee the pesticide mixture standard solution.
*2 The preparation concentration is converted to 20 µg/mL as a total of Orysastrobin and (5Z)-Orysastrobin (each 10 μg/mL).
*3 The preparation concentration is converted to 20 µg/mL as a total of E and Z isomer (each 10 μg/mL).

Analysis conditions

[ Instrument ]

Shimadzu LCMS-8040

[ HPLC ]

Column： Wakopak^® Wakosil-Ⅱ 3C18HG 2.0 × 150 mm

Column temperature： 40℃

Eluent： A) 5 mmol/L CH₃COONH₄ in H₂O, B) 5 mmol/L CH₃COONH₄ in CH₃OH

Eluent：

Time (min)	B conc. (%)
0-7	10-45
7-42	45-80
42-46	80-100
46-51	100

Flow rate： 0.2 mL/min

[ MS ]

Ionization： ESI

Mode： MRM

3 Pesticides Mixture Standard Solution WQ-10 (each 20 μg/mL Acetone Solution)

LC/MS Chromatogram [1]*

Peak No.	Constituent	Monitoring Ion (m/z)	Mode	Peak No.	Constituent	Monitoring Ion (m/z)	Mode	Peak No.	Constituent	Monitoring Ion (m/z)	Mode
1	DMTP Oxon (Methidathion Oxon)	287	+	2	Ipfencarbazone	427	+	3	Iprodione Metabolite	328	-

*Analysis conditions, charts, and detected ions are reference data. It is not the data to guarantee the pesticide mixture standard solution.

Analysis conditions

[ Instrument ]

Shimadzu LC/MS-8040

[ HPLC ]

Column： Wakopak^® Wakosil-Ⅱ 3C18HG 2.0 × 150 mm

Column temperature： 40℃

Eluent： A) 5 mmol/L CH₃COONH₄ in H₂O, B) 5 mmol/L CH₃COONH₄ in CH₃OH

Eluent：

Time (min)	B conc. (%)
0-20	5-100
20-40	100

Flow rate： 0.2 mL/min

[ MS ]

Ionization： ESI

LC/MS Chromatogram [2]*

Peak No.	Constituent	Monitoring Ion (m/z)			Mode	Peak No.	Constituent	Monitoring Ion (m/z)			Mode	Peak No.	Constituent	Monitoring Ion (m/z)			Mode
		Precursor	Product					Precursor	Product					Precursor	Product
1	DMTP Oxon (Methidathion Oxon)	287	145	85	+	2	Ipfencarbazone	427	198	128	+	3	Iprodione Metabolite	330	101	143	+

*Analysis conditions, charts, and detected ions are reference data. It is not the data to guarantee the pesticide mixture standard solution.

[Instrument]

HPLC： ACQUITY UPLC H-Class Plus System (post injector mixing kit: 50 μL)

MS： Xevo TQ-S micro

[HPLC]

Column temperature： 40℃

Eluent： A) 2 mmol/L CH₃COONH₄ in 0.05% HCOOH/H₂O, B) 2 mmol/L CH₃COONH₄ in 0.05% HCOOH/CH₃OH *Gradient

[MS]

Ionization： ESI

Mode： MRM