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VA-044, Azo Initiator

Wako 1st Grade
Specification Assay :
97.0+% (Gas Analysis)
Manufacturer :
FUJIFILM Wako Chemicals U.S.A. Corporation
Storage Condition :
Protect from light
Molecular Formula :
Molecular Weight :
  • Structural Formula
  • Label
  • Packing
Product Number
Package Size
50 G
List Price
45.00 USD

In stock

500 G
List Price
165.00 USD

Out of Stock


Spectral Data
Certificate of Analysis
Calibration Certificate

Application Data



CLALITY is the technique develped in the Deisseroth lab at Stanford University.
Chung, K. et al., Nature, 497, 332-337 (2013).
This protocol transforms intact tissues including whole organs into an optically transparent hydrogel-hybridized form, ready for molecular phenotyping and high resolution 3D imaging.

US00000003_img02.png*Data was provided by FUJIFILM Wako Pure Chemical Corporation.


  1. Chung, K., Wallace, J., Kim, S. Y., Kalyanasundaram, S., Andalman, A. S., Davidson, T. J., Mirzabekov, J. J., Zalocusky, K. A., Mattis, J., Denisin, A. K., Pak, S., Bernstein, H., Ramakrishnan, C., Grosenick, L., Gradinaru, V. and Deisseroth, K.: "Structural and molecular interrogation of intact biological systems". Nature, 497, 7449, 332-337 (2013). PMID: 23575631
  2. Chung K., and Deisseroth. K.: "CLALITY for mapping the nervous system". Nature, 10, 508-513 (2013). PMID: 23722210
  3. Underwood E.: "Neuroscience. New tools light up the intricacies of the brain". Science, 342, 917-918 (2013). PMID: 24264967
  4. Tomer, R., Ye, L., Hsueh, B., and Deisseroth, K.: " Adcanced CLARITY for rapid and high-resolution imaging of intact tissues. Nature Protocols, 9, 7, 1682-1697 (2014). PMID: 24945384
  5. Yang, B., Treweek, J. B., Kulkami, R. P., Deverman, B. E., Chen, C. K., Lubeck, E., Shah, S., Cai, L., and Gradinaru, V.: "Single-cell phenotyping withing transparent intact tissue through whole-body clearing". Cell, 158, 945-958 (2014). PMID: 25088144
  6. Ye. L., Allen, W. E., Thompson, K. R., Tian, Q., Hsueh, B., Ramakrishnan, C., Wang, A. C., Jennings, J. H., Adhikari, a., Halpern, C. H., Witten, I. B., Barth, A. L., Luo, L., McNab., J. A., and Deisseroth., K.: "Wiring and Molecular Features of Prefrontal Ensembles Representing Distinct Experiences". Cell, 165, 1776-1788 (2016). PMID: 27238022
  7. Ku., T., Swaney, J., Park, J. Y., Albanese, A., Murray, E., Cho, J. H. Park, Y. G., Mangena, V., Chen, J., and Chung, K.: "Multiplexed and scalable super-resolution imaging of three-dimensional protein localization in size-adjustable tissues". Nature Biotechnol., 34, 973-981 (2016). PMID: 27454740
  8. Kadoki, M., Patil., A., Thaiss, C. C., Brooks, D. J. Pandey, S., Deep, D., Alvarez, D., von Andrian, U. H., Wagers, A. J., Nakai, K., Mikkelsen, T. S., Soumillon, M., and Chevrier, N. " Organism-Level Analysis of Vaccination Reveals Networks of Protection across Tissues". Cell, 171, 398-413 (2017). PMID: 28942919

Video from Nature Journal

Watch this video to know more about CLARITY | Nature Video-YouTube

Overview / Applications

Outline VA-044 is a non-nitrile, cationic water-soluble azo polymerization initiator.
It is highly effective for the polymerization of water-soluble vinyl monomer.
The 10-hour half-lifetemperature is as low as 44 ºC, and the product is active at low temperatures.


Appearance white - light yellow
crystals or crystalline powder
Specificity 10-hour half life temperature: 44 ºC (Water)
Solubility water: free soluble.
methanol: sparingly soluble.
Ethanol: very slightly soluble.
acetone, toluene: insoluble.
Melting Point 188-193 °C

Manufacturer Information


  • 2,2'-Azobis[2-(2-imidazolin-2-yl)propane] Dihydrochloride

Author : [WKUS] Naomi Tsurutani

For research use or further manufacturing use only. Not for use in diagnostic procedures.

Product content may differ from the actual image due to minor specification changes etc.

If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.


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