LabAssay (TM) GLDH (AA-type)
- for Cellbiology
- Manufacturer :
- FUJIFILM Wako Pure Chemical Corporation
- Storage Condition :
- Keep at 2-10 degrees C.
- GHS :
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150Tests
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In stock in Japan |
※Check availability in the US with the distributor.
Document
Kit component
For 150 tests
| GLDH Standard | 2 bottles |
|---|---|
| Reaction Solution 1a (R1a) | 17.1 mL x 2 bottles |
| Reaction Solution 1b (R1b) | 2 bottles |
| Reaction Solution 2 (R2) | 15 mL |
Product Overview
GLDH is an essential enzyme to synthesize urea and it catalyzes the reversible conversion from glutamate to alpha-ketoglutarate.
Clinically GLDH is a deviation enzyme in blood which deviates from cells when the cells are damaged, only low level of GLDH is found in serum from healthy individuals. As GLDH is particularly abundant in hepatocytes, it is used as a marker for liver injury in toxicity studies and related applications.
LabAssay™ GLDH (AA-type) is a research reagent for measuring GLDH for use with automated analyzer in human, mouse, rat, dog, and rabbit serum and plasma. GLDH levels in samples can be measured quickly.
Kit Performance
| applicable specimen | Human (Serum, Plasma) Mouse (Serum, Plasma) Rat (Serum, Plasma) Dog (Serum, Plasma) Rabbit (Serum, Plasma) |
|---|---|
| Calibration curve range | 1.0-150 U/L |
| Sample volume | 15 μL (in case of LABOSPECT 003) |
| Measurement duration | Approx. 10 min |
| Wavelength | Primary wavelength 340 nm Reference wavelength 405 nm |
Compatible Automated Analyzers
- LABOSPECT 003 (Hitachi High-Tech) etc.
Please contact us for other compatible automated analyzers, assay parameters, and protocols.
Principle
GLDH in the sample catalyzes the conversion of ammonia and α-ketoglutarate to glutamate. During this reaction, NADH is oxidized to NAD+, resulting in a decrease in absorbance at 340 nm. The GLDH level is determined by measuring this decrease in absorbance.
Data
Rpeatability (within run precision)
Serum and EDTA-plasma samples at two different concentrations were each measured in eight replicates using the current product to verify precision.
| n \ ID | Human serum | Human plasma (EDTA) |
Mouse serum | Mouse plasma (EDTA) |
Rat serum | Rat plasma (EDTA) |
Dog serum | Dog plasma (EDTA) |
Rabbit serum | Rabbit plasma (EDTA) |
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ID1 (U/L) |
ID2 (U/L) |
ID1 (U/L) |
ID2 (U/L) |
ID1 (U/L) |
ID2 (U/L) |
ID1 (U/L) |
ID2 (U/L) |
ID1 (U/L) |
ID2 (U/L) |
ID1 (U/L) |
ID2 (U/L) |
ID1 (U/L) |
ID2 (U/L) |
ID1 (U/L) |
ID2 (U/L) |
ID1 (U/L) |
ID2 (U/L) |
ID1 (U/L) |
ID2 (U/L) |
|
| 1 | 57.3 | 13.6 | 44.0 | 11.1 | 50.1 | 10.5 | 47.7 | 4.4 | 54.8 | 14.1 | 50.9 | 16.1 | 42.8 | 11.5 | 42.8 | 15.5 | 51.4 | 11.9 | 59.2 | 14.9 |
| 2 | 57.7 | 13.2 | 43.8 | 11.1 | 49.9 | 10.1 | 48.1 | 4.5 | 54.1 | 15.0 | 51.2 | 15.7 | 43.3 | 11.8 | 43.3 | 15.0 | 51.8 | 11.8 | 59.2 | 15.6 |
| 3 | 57.9 | 12.6 | 43.8 | 11.0 | 50.1 | 10.4 | 47.9 | 4.2 | 52.8 | 13.4 | 50.3 | 16.0 | 43.2 | 11.5 | 43.2 | 15.7 | 51.8 | 12.2 | 59.5 | 15.5 |
| 4 | 57.4 | 12.6 | 43.7 | 11.1 | 49.5 | 10.4 | 47.8 | 4.3 | 53.4 | 13.7 | 50.9 | 16.0 | 43.4 | 11.5 | 43.4 | 15.4 | 51.5 | 11.9 | 59.9 | 15.6 |
| 5 | 57.7 | 12.8 | 43.8 | 10.9 | 49.7 | 10.1 | 47.8 | 4.3 | 54.9 | 13.3 | 50.9 | 15.9 | 43.2 | 11.3 | 43.2 | 13.9 | 51.7 | 11.9 | 60.0 | 15.5 |
| 6 | 57.1 | 13.0 | 43.7 | 10.9 | 50.0 | 10.5 | 47.6 | 4.7 | 53.9 | 14.4 | 50.6 | 16.2 | 43.5 | 12.0 | 43.5 | 15.0 | 51.7 | 11.5 | 60.1 | 15.0 |
| 7 | 57.3 | 12.8 | 43.6 | 10.2 | 51.5 | 10.4 | 47.8 | 4.6 | 54.0 | 14.9 | 50.7 | 16.0 | 43.5 | 11.8 | 43.5 | 14.3 | 51.6 | 12.1 | 59.6 | 15.6 |
| 8 | 57.4 | 12.6 | 43.6 | 10.7 | 40.8 | 10.4 | 48.5 | 4.4 | 54.3 | 13.6 | 51.2 | 15.7 | 43.6 | 11.8 | 43.6 | 15.0 | 51.9 | 11.9 | 59.8 | 15.2 |
| Average | 57.5 | 12.9 | 43.8 | 10.9 | 49.0 | 10.4 | 47.9 | 4.4 | 54.0 | 14.1 | 50.8 | 16.0 | 43.3 | 11.7 | 43.3 | 15.0 | 51.7 | 11.9 | 59.7 | 15.4 |
| SD | 0.27 | 0.35 | 0.13 | 0.31 | 3.3 | 0.16 | 0.28 | 0.17 | 0.69 | 0.66 | 0.30 | 0.18 | 0.25 | 0.23 | 0.25 | 0.61 | 0.17 | 0.21 | 0.35 | 0.29 |
| CV (%) | 0.46 | 2.7 | 0.30 | 2.8 | 6.8 | 1.5 | 0.59 | 3.8 | 1.3 | 4.7 | 0.59 | 1.1 | 0.58 | 2.0 | 0.58 | 4.1 | 0.32 | 1.7 | 0.58 | 1.9 |
[Result]
All samples showed acceptable CV% values.
Reproducibility (between run precision)
Serum and EDTA-plasma samples at two different concentrations were measured over four consecutive days to verify reproducibility.
| Day \ ID | Human serum | Human plasma (EDTA) |
Mouse serum | Mouse plasma (EDTA) |
Rat serum | Rat plasma (EDTA) |
Dog serum | Dog plasma (EDTA) |
Rabbit serum | Rabbit plasma (EDTA) |
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ID1 (U/L) |
ID2 (U/L) |
ID1 (U/L) |
ID2 (U/L) |
ID1 (U/L) |
ID2 (U/L) |
ID1 (U/L) |
ID2 (U/L) |
ID1 (U/L) |
ID2 (U/L) |
ID1 (U/L) |
ID2 (U/L) |
ID1 (U/L) |
ID2 (U/L) |
ID1 (U/L) |
ID2 (U/L) |
ID1 (U/L) |
ID2 (U/L) |
ID1 (U/L) |
ID2 (U/L) |
|
| 1 | 50.8 | 2.5 | 54.0 | 6.2 | 44.7 | 3.5 | 48.2 | 5.8 | 54.2 | 5.4 | 37.4 | 2.7 | 49.9 | 5.9 | 49.3 | 2.7 | 46.1 | 3.2 | 37.7 | 4.6 |
| 2 | 51.1 | 2.4 | 54.9 | 6.6 | 44.7 | 3.2 | 51.2 | 5.7 | 52.8 | 5.7 | 39.5 | 2.6 | 49.1 | 5.9 | 49.2 | 2.9 | 45.9 | 3.1 | 38.1 | 4.4 |
| 3 | 48.3 | 2.6 | 51.4 | 6.0 | 43.4 | 3.3 | 48.2 | 5.6 | 52.3 | 5.7 | 36.7 | 2.7 | 48.5 | 5.9 | 47.8 | 2.7 | 44.7 | 3.4 | 36.7 | 4.2 |
| 4 | 49.7 | 2.8 | 53.7 | 6.3 | 45.5 | 3.4 | 47.5 | 5.4 | 54.8 | 5.4 | 38.0 | 2.8 | 50.8 | 6.2 | 50.0 | 2.9 | 47.2 | 2.9 | 39.0 | 4.5 |
| Average | 50.0 | 2.6 | 53.5 | 6.3 | 44.6 | 3.4 | 48.8 | 5.6 | 53.5 | 5.6 | 37.9 | 2.7 | 49.6 | 6.0 | 49.1 | 2.8 | 46.0 | 3.2 | 37.9 | 4.4 |
| SD | 1.3 | 0.17 | 1.5 | 0.25 | 0.87 | 0.13 | 1.7 | 0.17 | 1.2 | 0.17 | 1.2 | 0.082 | 1.0 | 0.15 | 0.92 | 0.12 | 1.0 | 0.21 | 0.95 | 0.17 |
| CV (%) | 2.5 | 6.6 | 2.8 | 4.0 | 2.0 | 3.9 | 3.4 | 3.0 | 2.2 | 3.1 | 3.1 | 3.0 | 2.0 | 2.5 | 1.9 | 4.1 | 2.2 | 6.6 | 2.5 | 3.9 |
[Result]
All samples showed acceptable CV% values.
Dilution linearity test
Samples at two different concentrations were serially diluted two-fold and measured in duplicate to verify linearity.
[Result]
All samples demonstrated good linearity within the measurement range.
Spiked recovery test
Standard solutions at four concentrations were added to serum/plasma (EDTA) from humans, mice, rats, dogs and rabbits. Next, spike-recovery tests were conducted, with each measurement performed in duplicate.
Human
| Amount spiked (U/L) | Measured value (U/L) | Recovery volume (U/L) | Recovery rate (%) | |
|---|---|---|---|---|
| Serum | 0.0 | 5.9 | - | - |
| 5.0 | 10.6 | 4.7 | 94.0 | |
| 10.0 | 15.7 | 9.8 | 98.0 | |
| 25.0 | 31.5 | 25.6 | 102 | |
| 50.0 | 58.2 | 52.3 | 105 | |
| Plasma (EDTA) | 0.0 | 1.1 | - | - |
| 5.0 | 6.1 | 5.0 | 100 | |
| 10.0 | 10.9 | 9.8 | 98.0 | |
| 25.0 | 27.5 | 26.4 | 106 | |
| 50.0 | 52.8 | 51.7 | 103 |
Mouse
| Amount spiked (U/L) | Measured value (U/L) | Recovery volume (U/L) | Recovery rate (%) | |
|---|---|---|---|---|
| Serum | 0.0 | 6.3 | - | - |
| 5.0 | 11.2 | 4.9 | 98.0 | |
| 10.0 | 16.1 | 9.8 | 98.0 | |
| 25.0 | 30.9 | 24.6 | 98.4 | |
| 50.0 | 58.4 | 52.1 | 104 | |
| Plasma (EDTA) | 0.0 | 19.0 | - | - |
| 5.0 | 24.0 | 5.0 | 100 | |
| 10.0 | 29.0 | 10.0 | 100 | |
| 25.0 | 44.8 | 25.8 | 103 | |
| 50.0 | 70.6 | 51.6 | 103 |
Rat
| Amount spiked (U/L) | Measured value (U/L) | Recovery volume (U/L) | Recovery rate (%) | |
|---|---|---|---|---|
| Serum | 0.0 | 13.0 | - | - |
| 5.0 | 18.2 | 5.2 | 104 | |
| 10.0 | 23.4 | 10.4 | 104 | |
| 25.0 | 39.5 | 26.5 | 106 | |
| 50.0 | 67.1 | 54.1 | 108 | |
| Plasma (EDTA) | 0.0 | 2.8 | - | - |
| 5.0 | 8.3 | 5.5 | 110 | |
| 10.0 | 13.7 | 10.9 | 109 | |
| 25.0 | 30.4 | 27.6 | 110 | |
| 50.0 | 57.4 | 54.6 | 109 |
Dog
| Amount spiked (U/L) | Measured value (U/L) | Recovery volume (U/L) | Recovery rate (%) | |
|---|---|---|---|---|
| Serum | 0.0 | 9.4 | - | - |
| 5.0 | 14.5 | 5.1 | 102 | |
| 10.0 | 19.8 | 10.4 | 104 | |
| 25.0 | 34.7 | 25.3 | 101 | |
| 50.0 | 61.4 | 52.0 | 104 | |
| Plasma (EDTA) | 0.0 | 4.1 | - | - |
| 5.0 | 9.3 | 5.2 | 104 | |
| 10.0 | 14.5 | 10.4 | 104 | |
| 25.0 | 29.9 | 25.8 | 103 | |
| 50.0 | 56.4 | 52.3 | 105 |
Rabbit
| Amount spiked (U/L) | Measured value (U/L) | Recovery volume (U/L) | Recovery rate (%) | |
|---|---|---|---|---|
| Serum | 0.0 | 11.6 | - | - |
| 5.0 | 16.8 | 5.2 | 104 | |
| 10.0 | 21.7 | 10.1 | 101 | |
| 25.0 | 36.5 | 24.9 | 99.6 | |
| 50.0 | 62.8 | 51.2 | 102 | |
| Plasma (EDTA) | 0.0 | 19.6 | - | - |
| 5.0 | 24.7 | 5.1 | 102 | |
| 10.0 | 29.7 | 10.1 | 101 | |
| 25.0 | 45.3 | 25.7 | 103 | |
| 50.0 | 71.0 | 51.4 | 103 |
[Result]
Good recovery rate was confirmed.
Correlation with the Previous Version of the Kit
GLDH levels in rat serum and plasma were measured using LabAssay™ GLDH (AA-type) (hereinafter, this assay kit) and LabAssay™ GLDH-Rat (A-type) (Code No. 291-93401, hereinafter, the previous kit), and the correlation between the two products was evaluated.
[Result]
A strong correlation was confirmed between the measurements obtained using the current and previous kit.
Correlation with products from other manufacturers
GLDH levels in serum and plasma from humans, mice, rats, dogs, and rabbits were measured using LabAssay™ GLDH (AA-type) (hereinafter, this assay kit) and assay reagents from Company A and Company B. The correlations between the measurement results of each product were evaluated.
[Result]
A strong correlation was confirmed between the measurements obtained using this assay kit and other manufacturers’ products.
Measurement Using Actual Samples
| Animal | Sample* | Measured value (U/L) |
|---|---|---|
| Human | Serum | 5.9 |
| Plasma (EDTA) | 1.1 | |
| Mouse (BALB/c, ♂) |
Serum | 6.3 |
| Plasma (EDTA) | 19.0 | |
| Rat (SD) |
Serum | 13.0 |
| Plasma (EDTA) | 2.8 | |
| Dog (Beagle) |
Serum | 9.4 |
| Plasma (EDTA) | 4.1 | |
| Rabbit (Japanese White Rabbit) |
Serum | 11.6 |
| Plasma (EDTA) | 19.6 |
FAQ
About sample
- How should samples be stored after collection?
- Measure immediately after blood collection or freeze at -35℃ or below for long-term storage.
- What should I do with samples that exceed the measurable range?
- Dilute specimen with saline and repeat the assay if the measured value exceeds the measurable range, and multiply the result by the dilution factor.
- Is there any influence from substances that interfere with measurement (hemolysis, chyle, ascorbic acid, bilirubin, etc.)?
Please treat the measured values of hemolyzed samples as reference values. Chyle, ascorbic acid, and bilirubin have almost no effect on the measured values. We have confirmed that there is no influence at the following concentrations.
Effects of interfering substances
Hemolysis No influence up to 100 mg/dL Chyle (Formazine Turbidity Unit) No influence up to 500 FTU Ascorbic acid No influence up to 50 mg/dL Bilirubin-F No influence up to 50 mg/dL Bilirubin-C No influence up to 20 mg/dL
About kit usage
- What instruments, and equipment are required for the assay using this kit?
- The instruments and equipment required for the use of this kit are listed below.
- Automated analyzer
- Pipettes with replaceable tips
- Vortex mixer
- Purified water (distilled water)
- Saline slution
- What are the storage conditions and shelf-life guidelines for each reagent?
GLDH Standard
Unopened standards should be stored at 2–10°C. Use immediately after reconstitution. In our internal study, the measurement value changed by -1.6% at room temperature and -5.3% at 4°C after 24 hours following reconstitution.Reaction Solution 1a (R1a)
Store at 2-10°C.Reaction Reagent 1b (R1b)
Store unused Reaction Reagent 1b (R1b) at 2-10°C.Mixed Reaction Reagent 1 (R1a+R1b)
After preparation, store at 2–10°C and use within 3 weeks.Reaction Reagent 2 (R2)
After preparation, store at 2–10°C and use within 3 weeks.The above are general guidelines; actual stability may vary depending on storage conditions. For example, if reagents are left in an automated analyzer after measurement, their usable period may be shortened depending on the analyzer’s operating status. When not in use, remove the reagents from the analyzer, cap them securely, and store as directed.
- Is control serum available?
- Yes, we provide control serum (Code No. 128-07131) for use in quality control.
- What is the amount of purified water to be added to the standard product?
- Is there any influence from substances that interfere with measurement (hemolysis, chyle, ascorbic acid, bilirubin, etc.)?
Overview / Applications
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Manufacturer Information
Alias
For research use or further manufacturing use only. Not for use in diagnostic procedures.
Product content may differ from the actual image due to minor specification changes etc.
If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.