microRNA ExtractorR Kit for Purified EV
- for Genetic Research
- Manufacturer :
- FUJIFILM Wako Pure Chemical Corporation
- Storage Condition :
- Keep at 2-10 degrees C.
- GHS :
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20Tests
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In stock in Japan |
※Check availability in the US with the distributor.
Document
Kit component
Kit Component
| Lysis Solution | 9 mL |
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| Reducing Agent | 80 μL |
| Protease Solution | 200 μL |
| Enhancer | 200 μL |
| Wash Solution Ⅰ | 5 mL |
| Wash Solution Ⅱ | 5 mL × 2 tubes |
| Elution Buffer | 1 mL × 2 tubes |
| Spin Column | 20 tubes |
| Collection Tube | 20 tubes |
Product Overview
microRNA Extractor® Kit for Purified EV is designed for extraction of total RNA, including microRNA, from extracellular vesicles (EV), including exosomes, using spin columns. High concentrations of RNA can be recovered with a small amount of elution buffer (20 μL), making it suitable for analyses such as next-generation sequencing (NGS) etc.
Features
- High concentrations of RNA can be recovered with a small amount of elution buffer (20 μL)
- Optimized for RNA extraction from purified exosomes
- Higher yield of RNA compared to conventional products of other companies
Application
| Sample | Exosomal solutions purified from cell culture supernatants, serum, and plasma [Note] It has not been confirmed whether it can be directly extracted from cell culture supernatant, serum or plasma. |
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| Application | RT-qPCR, Microarray, NGS, etc. |
Protocol
[Protocol] microRNA Extractor® Kit for Purified EV (Youtube, 5:02)
Data
Performance Data
Analysis of Exosomal miRNA by NGS
RNA was extracted from the exosomes isolated using MagCapture™ Exosome Isolation Kit PS Ver.2 and analyzed by NGS.
[Result] More miRNAs were detected with Fujifilm Wako’s product compared to the conventional products of other companies.
Comparison of RNA yield with conventional products
Exosomes were isolated from umbilical cord-derived mesenchymal stem cells using the MagCapture™ Exosome Isolation Kit PS Ver.2, and RNA was extracted using three different kits. RNA was measured by QuantiFluor® RNA System (Promega Corporation) and RT-qPCR.
QuantiFluor® is a trademark or registered trademark of Promega Corporation.
[Result] Fujifilm Wako’s product showed higher RNA extraction efficiency than the conventional products of other companies.
Overview / Applications
| Outline | microRNA Extractor Kit for Purified EV is a kit for extraction of total RNA, including microRNA from purified EV derived from cell culture supernatant and brood (serum and plasma). This kit is optimized to extract RNA from purified EVs. There is no need for phenol and chloroform to use this kit. The extracted RNA by this kit can use various downstream analyses such as NGS, microarray, and RT-qPCR. |
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Property
Manufacturer Information
Alias
- microRNA Extractor (R) Kit for Purified Exosome
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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