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microRNA Extractor® Kit for Purified EV

for Genetic Research
Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at 2-10 degrees C.
GHS :
  • Structural Formula
  • Label
  • Packing
SDS
Comparison
Product Number
Package Size
Price
Inventory
Distributor
294-84601
Barcode No
4548995090782
20Tests
List Price
440.00 USD

In stock in Japan

Document

SDS
Product Specification Sheet
Package Insert
Spectral Data
Certificate of Analysis
Calibration Certificate

Kit component

Kit Component

Lysis Solution 9 mL
Reducing Agent 80 μL
Protease Solution 200 μL
Enhancer 200 μL
Wash Solution Ⅰ 5 mL
Wash Solution Ⅱ 5 mL × 2 tubes
Elution Buffer 1 mL × 2 tubes
Spin Column 20 tubes
Collection Tube 20 tubes

Product Overview

microRNA Extractor® Kit for Purified EV is designed for extraction of total RNA, including microRNA, from extracellular vesicles (EV), including exosomes, using spin columns. High concentrations of RNA can be recovered with a small amount of elution buffer (20 μL), making it suitable for analyses such as next-generation sequencing (NGS) etc.

Features

  • High concentrations of RNA can be recovered with a small amount of elution buffer (20 μL)
  • Optimized for RNA extraction from purified exosomes
  • Higher yield of RNA compared to conventional products of other companies

Application

Sample Exosomal solutions purified from cell culture supernatants, serum, and plasma
[Note] It has not been confirmed whether it can be directly extracted from cell culture supernatant, serum or plasma.
Application RT-qPCR, Microarray, NGS, etc.

Protocol

04417497_img01.png

 


[Protocol] microRNA Extractor® Kit for Purified EV (Youtube, 5:02)

Data

Performance Data

Analysis of Exosomal miRNA by NGS

RNA was extracted from the exosomes isolated using MagCapture™ Exosome Isolation Kit PS Ver.2 and analyzed by NGS.

04417497_img02.png

[Result] More miRNAs were detected with Fujifilm Wako’s product compared to the conventional products of other companies.

 

Comparison of RNA yield with conventional products

Exosomes were isolated from umbilical cord-derived mesenchymal stem cells using the MagCapture™ Exosome Isolation Kit PS Ver.2, and RNA was extracted using three different kits. RNA was measured by QuantiFluor® RNA System (Promega Corporation) and RT-qPCR.

QuantiFluor® is a trademark or registered trademark of Promega Corporation.

04417497_img03.png

[Result] Fujifilm Wako’s product showed higher RNA extraction efficiency than the conventional products of other companies.

Overview / Applications

Property

Manufacturer Information

Alias

  • microRNA Extractor® Kit for Purified Exosome

For research use or further manufacturing use only. Not for use in diagnostic procedures.

Product content may differ from the actual image due to minor specification changes etc.

If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.

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