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Oxytocin ELISA Kit Wako

for Immunochemistry
Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at 2-10 degrees C.
GHS :
  • Structural Formula
  • Label
  • Packing
SDS
Comparison
Product Number
Package Size
Price
Inventory
Distributor
292-84401
Barcode No
4548995087829
96Tests
List Price
1,078.00 USD

In stock in Japan

Document

SDS
Product Specification Sheet
Package Insert
Spectral Data
Certificate of Analysis
Calibration Certificate

Kit component

96 tests

Antibody-coated Plate 1 plate
Oxytocin Standard 100 μL
Buffer 60 mL
Biotin-conjugated Oxytocin 1 bottle
Peroxidase-conjugated Streptavidin Solution 100 μL
Luminescent Reagent 1 6 mL
Luminescent Reagent 2 6 mL
Wash Solution (10×) 100 mL
Sample Buffer 1 5 mL
Sample Buffer 2 12 mL
Plate Seal 4 sheets

Product Information

Oxytocin is a peptide hormone that comprises nine amino acids. It is often called the "happy hormone" or "love hormone", as it has stress-relieving, anti-anxiety, and fear-alleviating effects and plays a role in the development of maternal behavior. It is a molecule that has attracted attention in treating mental disorders such as depression and autism or developing functional food materials. However, the assay of oxytocin used to include the need for complicated pretreatment using a C18 column, and a large amount of sample.

Oxytocin ELISA Kit Wako is an ELISA kit that can determine the amount of oxytocin in a sample.It overcomes issues with conventional oxytocin assays, since sample pretreatment simply requires mixing, stirring, and centrifuging of reagents, and the minimum sample amount required is 50 μL.

Features

  • Easy-to-use pretreatment
    Sample pretreatment requires simply adding pretreatment solutions, stirring, and centrifuging. A C18 column and organic solvent are not required.
  • Can be assayed with a tiny sample volume
    Minimum sample amount required is 50 μL (n=1).
  • Short assay time
    Assay time is 2.5 hours. Pretreatment can be done in approximately 30 minutes.
  • Can be used with a variety of samples
    Human saliva/urine/serum/plasma
    Mouse or rat serum/plasma
[Requirements before your purchase]

As this product uses a competitive assay with high sensitivity, a plate shaker and a plate reader for luminescence detection are required.

  • Kit Performance

    The range of the calibration curve 4.00~1,024 pg/mL
    Assay target Oxytocin
    Sample Human saliva/urine/serum/plasma
    Mouse serum/plasma
    Rat serum/plasma
    The sample amount required 50 μL (Required amount for n=1)
    200 μL (Recommended amount for n=2)
    Assay time Approximately 2.5 hours
    Detection method Luminescence (a plate reader for luminescence detection is required)
  • Example of Calibration Curve

    04173912_img01.png
  • Assay Principles

    This kit is based on a competitive assay, and luminescence intensity decreases as the amount of oxytocin in the sample increases.

    04173912_img02.png
  • 1. In advance, anti-oxytocin antibody, rabbit polyclonal antibody, and anti-rabbit secondary antibody are solid phased in each well of the assay plate.
    2. Add oxytocin-biotin and the standard solution or the pretreated sample to wells of the assay plate. Allow them to react competitively.
    3. Allow biotin and HRP-conjugated streptavidin to react.
    4. Add a luminescent substrate for HRP.
    5. Assay the oxytocin concentration in the sample by measuring HRP activity (luminescence intensity) in the wells.

Sample Pretreatment Methods

  • Pretreatment method of FUJIFILM Wako
    (approximately 30 min, recovery rate: 90 – 120%)

    Sample (saliva/urine/serum/plasma)

    Add Sample Buffer 1

    After stirring, allow to stand at room temperature for 10 minutes (stir once after 5 minutes have elapsed)

    Add Sample Buffer 2

    After stirring, allow to stand at room temperature for 10 minutes (stir once after 5 minutes have elapsed)

    Centrifuge

    Samples to be measured consist of separated supernatant

  • Pretreatment method of competitive company E
    (approximately 2 – 4 hours, recovery rate: 42 – 110%)

    Sample (saliva/urine/serum/plasma)

    Add trifluoroacetic acid (TFA), and stir

    Centrifuge

    Add separated supernatant to a C18 column that was equilibrated with TFA and acetonitrile

    Wash with TFA and elute with acetonitrile

    Decompress and dry the elution fraction

    Dissolve with buffer to make samples to be measured

Data

Spiked recovery test

Human

  Spiked Oxytocin
(pg/mL)
Assay value
(pg/mL)
Recovery volume
(pg/mL)
Recovery
(%)
Urine 1 0 31.6 - -
100 125 93.4 93.4
Urine 2 0 52.9 - -
200 250 197 98.5
Spiked Oxytocin
(pg/mL)
Assay value
(pg/mL)
Recovery volume
(pg/mL)
Recovery
(%)
Serum 1 0 19.7 - -
100 112 92.3 92.3
Serum 2 0 30.2 - -
200 211 181 90.5
Spiked Oxytocin
(pg/mL)
Assay value
(pg/mL)
Recovery volume
(pg/mL)
Recovery
(%)
Saliva 1 0 77.7 - -
100 167 89.3 89.3
Saliva 2 0 27.6 - -
200 222 194 97.0

Mouse

Spiked Oxytocin
(pg/mL)
Assay value
(pg/mL)
Recovery volume
(pg/mL)
Recovery
(%)
Serum 0 49.7 - -
50 107 57.3 115
100 152 102 102
250 305 255 102
500 586 536 107
Average 107
Spiked Oxytocin
(pg/mL)
Assay value
(pg/mL)
Recovery volume
(pg/mL)
Recovery
(%)
Plasma 0 61.8 - -
50 110 48.2 96.4
100 156 94.2 94.2
250 320 258 103
500 595 533 107
Average 100

Rat

Spiked Oxytocin
(pg/mL)
Assay value
(pg/mL)
Recovery volume
(pg/mL)
Recovery
(%)
Serum 0.00 18.8 - -
50.0 83.0 64.2 128
100 136 117 117
250 289 271 108
500 535 516 103
Average 114
Spiked Oxytocin
(pg/mL)
Assay value
(pg/mL)
Recovery volume
(pg/mL)
Recovery
(%)
Plasma 0.00 60.1 - -
50.0 110 49.9 99.8
100 167 107 107
250 335 274 110
500 573 513 103
Average 105

Overview / Applications

Outline Oxytocin is a peptide hormone consisting of 9 amino acids. It is produced in the hypothalamus and released mainly from the posterior pituitary gland in the wake of childbirth and lactation, promotes uterine contraction and milk production. Oxytocin is commonly called happiness hormone or love hormone because it is involved in behavior formation and anti-stress action. This product is an ELISA kit that can measure oxytocin in a sample. This kit can be measured by a simpler pretreatment without C18 column and organic solvent than conventionally pretreatment method.

Calibration curve range: 4-1,024 pg/mL
Sample: human saliva, urine, plasma, serum, mouse serum, plasma, rat plasma, serum
Sample volume: 50microL (minimum at n=1)
200microL (recommended at n=2)
Assay time: 2.5 hours
Detection method: luminescence detection

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Manufacturer Information

Alias

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