Oxytocin ELISA Kits
Oxytocin is a peptide hormone that comprises nine amino acids. It is often called the "happy hormone" or "love hormone", as it has stress-relieving, anti-anxiety, and fear-alleviating effects and plays a role in the development of maternal behavior. It is a molecule that has attracted attention in treating mental disorders such as depression and autism or developing functional food materials. However, the assay of oxytocin used to include the need for complicated pretreatment using a C18 column, and a large amount of sample.
Oxytocin ELISA Kit Wako
Oxytocin ELISA Kit Wako is an ELISA kit that can determine the amount of oxytocin in a sample. It overcomes issues with conventional oxytocin assays, since sample pretreatment simply requires mixing, stirring, and centrifuging of reagents, and the minimum sample amount required is 50 μL.
Features
- Easy-to-use pretreatment
Sample pretreatment requires simply adding pretreatment solutions, stirring, and centrifuging. A C18 column and organic solvent are not required. - Can be assayed with a tiny sample volume
Minimum sample amount required is 50 μL (n=1). - Short assay time
Assay time is 2.5 hours. Pretreatment can be done in approximately 30 minutes. - Can be used with a variety of samples
Human saliva/urine/serum/plasma
Mouse or rat serum/plasma
[Requirements before your purchase]
As this product uses a competitive assay with high sensitivity, a plate shaker and a plate reader for luminescence detection are required.
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Kit Performance
The range of
the calibration curve4.00～1,024 pg/mL Assay target Oxytocin Sample Human saliva/urine/serum/plasma
Mouse serum/plasma
Rat serum/plasmaThe sample amount required 50 μL (Required amount for n=1)
200 μL (Recommended amount for n=2)Assay time Approximately 2.5 hours Detection method Luminescence (a plate reader for luminescence detection is required) -
Assay Principle
This kit is based on a competitive assay, and luminescence intensity decreases as the amount of oxytocin in the sample increases.
Sample Pretreatment Methods
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Pretreatment method of FUJIFILM Wako
(approximately 30 min, recovery rate: 90 – 120%)Sample (saliva/urine/serum/plasma)
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Add Sample Buffer 1
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After stirring, allow to stand at room temperature for 10 minutes (stir once after 5 minutes have elapsed)
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Add Sample Buffer 2
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After stirring, allow to stand at room temperature for 10 minutes (stir once after 5 minutes have elapsed)
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Centrifuge
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Samples to be measured consist of separated supernatant -
Pretreatment method of competitive company E
(approximately 2 – 4 hours, recovery rate: 42 – 110%)Sample (saliva/urine/serum/plasma)
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Add trifluoroacetic acid (TFA), and stir
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Centrifuge
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Add separated supernatant to a C18 column that was equilibrated with TFA and acetonitrile
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Wash with TFA and elute with acetonitrile
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Decompress and dry the elution fraction
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Dissolve with buffer to make samples to be measured
Resources
▼ Poster
| Title | Conference | Year | Language | Data |
|---|---|---|---|---|
| Development of a novel highly sensitive oxytocin ELISA | Neuroscience 2023 | 2023 | English |
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Oxytocin ELISA Kit
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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