Oxytocin ELISA Kits

Oxytocin ELISA Kit Wako is an ELISA kit that can determine the amount of oxytocin in a sample. It overcomes issues with conventional oxytocin assays, since sample pretreatment simply requires mixing, stirring, and centrifuging of reagents, and the minimum sample amount required is 50 μL.

Features

• Easy-to-use pretreatment
  Sample pretreatment requires simply adding pretreatment solutions, stirring, and centrifuging. A C18 column and organic solvent are not required.
• Can be assayed with a tiny sample volume
  Minimum sample amount required is 50 μL (n=1).
• Short assay time
  Assay time is approximately 2.5 hours. Pretreatment can be done in approximately 30 minutes.
• Can be used with a variety of samples
  Human saliva/urine/serum/plasma
  Mouse or rat serum/plasma

[Requirements before your purchase]

As this product uses a competitive assay with high sensitivity, a plate shaker and a plate reader for luminescence detection are required.

• Kit Performance

  Calibration curve range	4.00-12,500 pg/mL
  Assay target	Oxytocin
  Analysis sample	Human saliva/urine/serum/plasma Mouse serum/plasma Rat serum/plasma
  Sample volume	50 μL (Required amount for n=1) 200 μL (Recommended amount for n=2)
  Measurement duration	Approximately 2.5 hours
  Detection method	Luminescence (a plate reader for luminescence detection is required)

• Assay Principle

  This kit is based on a competitive assay, and luminescence intensity decreases as the amount of oxytocin in the sample increases.

  

Sample Pretreatment Methods

• Pretreatment method of FUJIFILM Wako
  (approximately 30 minutes)

  Sample (saliva/urine/serum/plasma)
  ↓
  Add Sample Buffer 1
  ↓
  After stirring, allow to stand at room temperature for 10 minutes (stir once after 5 minutes have elapsed)
  ↓
  Add Sample Buffer 2
  ↓
  After stirring, allow to stand at room temperature for 10 minutes (stir once after 5 minutes have elapsed)
  ↓
  Centrifuge
  ↓
  Samples to be measured consist of separated supernatant

• Pretreatment method of competitive company E
  (approximately 2 - 4 hours)

  Sample (saliva/urine/serum/plasma)
  ↓
  Add trifluoroacetic acid (TFA), and stir
  ↓
  Centrifuge
  ↓
  Add separated supernatant to a C18 column that was equilibrated with TFA and acetonitrile
  ↓
  Wash with TFA and elute with acetonitrile
  ↓
  Decompress and dry the elution fraction
  ↓
  Dissolve with buffer to make samples to be measured
  
  

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