Anti DYKDDDDK tag, Monoclonal Antibody
- for Immunochemistry
- Manufacturer :
- FUJIFILM Wako Pure Chemical Corporation
- Storage Condition :
- Keep at -20 degrees C.
- Host :
- Mouse
- Clone No. :
- 1E6
- Classification :
- Monoclonal
- Application :
- WB,IP
- GHS :
-
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- Structural Formula
- Label
- Packing
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Comparison
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1mg
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In stock in Japan |
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5mg
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In stock in Japan |
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200ug
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In stock in Japan |
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Document
Application
Overview / Applications
| Outline | The DYKDDDDK was consisted of 9 amino acids and has been used as the tag for detection and/or purification of recombinant protein. Monoclonal anti DYKDDDDK tag antibody was established by fusing mouse myeloma cells and spleen cells of mice immunized with synthetic DYKDDDDK peptide which was coupled with keyhole limpet hemocyanin (KLH). This antibody was purified from mouse ascites. This product is for research use only. Do not administer it to human. [Concentration] [Formulation] [Clone No.] [Subclass] [Specificity] [Working Dilution] |
|---|---|
| Features | Applicable to Western Blot analysis and Immunoprecipitation |
| Preparation | This antibody was purified from mouse ascites by using Protein A affinity chromatography. |
| Instructions | Western Blot Analysis (1 : 1,000 - 1 : 10,000) Immunoprecipitation (5 μg/20 μL 10% Protein A or Protein G beads slurry) |
Property
| Appearance | Liquid |
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| Composition | 1xPBS aqueous solution with 50% glycerol. |
| Concentration | 0.4 - 0.6 mg/mL Protein |
Antibody Information
| Antigen | DYKDDDDK tag |
|---|---|
| Antigen Alias | DYKDDDDK Peptide DYKDDDDK DYKDDDDK-tag DYKDDDDK Epitope Tag FLAG EPITOPE TAG Ftag Antibody DYKDDDDK FLAG FLAG Epitope FLAG peptide Flag-tag |
Manufacturer Information
Alias
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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