Anti SQSTM1/A170/p62, Rabbit

Autophagy Research

Sequestosome 1 (SQSTM1)/A170 (mouse)/p62 (human)/ZIP (rat) a ubiquitin-binding protein, expresses oxidative stress-dependently. Abnormality of SQSTM1 leads to bone metabolic disorder, obesity and Type II diabetes. SQSTM1 was reported to bind LC3, which regulates autophagosome formation. The protein has attracted the attention of researchers because it is believed to induce a protein from ubiquitin-proteosome system (UPS) to the lysosome-dependent macroautophagy (autophagy) system, which are two major intracellular pathways for protein degradation. Wako launched the mouse SQSTM1 (A170) rabbit antiserum, which is applicable to Western blot, immunohistochemistry and immunofluorescence.

Preparation
The antiserum is diluted with an equal amount of PBS and absorbed with E. coli proteins.
Immunogen
recombinant murine SQSTM1 (A170)(AA254-333) containing T7 tag at the N-terminal end and His tag at the carboxy-terminal end
Specificity
Specific for mouse and rat SQSTM1 (A170/ZIP).
Slightly reactive with human SQSTM1 (p62).
Working Dilution

• Western blot 1 : 200


• Immunohistochemistry 1 : 1,000


• Immuno fluorescence 1 : 1,000

References

1. Ishii, T., Yanagawa, T., Kawane, T., Yuki, K., Seita, J., Yoshida, H. and Bannai, S.: "Transcription factor Nrf2 coordinately regulates a group of oxidative stress-inducible genes in macrophages", Biochem. Biophys. Res. Commun., 226, 456-60 (1996).


2. Ishii, T., Itoh, K., Takahashi, S., Sato, H., Yanagawa, T., Katoh, Y., Bannai, S. and Yamamoto, M.: "Transcription factor Nrf2 coordinately regulates a group of oxidative stress-inducible genes in macrophages", J. Biol. Chem., 275, 16023-9 (2000).


3. Komatsu, M., Waguri, S., Koike, M., Sou, Y.S., Ueno, T., Hara, T., Mizushima, N., Iwata, J., Ezaki, J., Murata, S., Hamazaki, J., Nishito, Y., Iemura, S., Natsume, T., Yanagawa, T., Uwayama, J., Warabi, E., Yoshida, H., Ishii, T., Kobayashi, A., Yamamoto, M., Yue, Z., Uchiyama, Y., Kominami, E. and Tanaka, K.: "Homeostatic levels of p62 control cytoplasmic inclusion body formation in autophagy-deficient mice", Cell, 131, 1149-63 (2007).


4. Nakaso, K., Kitayama, M., Ishii, T., Bannai, S., Yanagawa, T., Kimura, K., Nakashima, K., Ohama, E. and Yamada, K.: "Effects of kainate-mediated excitotoxicity on the expression of rat counterparts of A170 and MSP23 stress proteins in the brain", Brain Res. Mol. Brain Res., 69, 155-63 (1999).