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Anti SQSTM1/A170/p62, Rabbit

for Immunochemistry
Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at -20 degrees C.
Source :
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List Price
292.00 USD

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Product Specification Sheet
Package Insert
Spectral Data
Certificate of Analysis
Calibration Certificate

Overview / Applications

Outline This product is for research use only. Do not administer it to human.

Autophagy Research

Sequestosome 1 (SQSTM1)/A170 (mouse)/p62 (human)/ZIP (rat) a ubiquitin-binding protein, expresses oxidative stress-dependently. Abnormality of SQSTM1 leads to bone metabolic disorder, obesity and Type II diabetes. SQSTM1 was reported to bind LC3, which regulates autophagosome formation. The protein has attracted the attention of researchers because it is believed to induce a protein from ubiquitin-proteosome system (UPS) to the lysosome-dependent macroautophagy (autophagy) system, which are two major intracellular pathways for protein degradation. Wako launched the mouse SQSTM1 (A170) rabbit antiserum, which is applicable to Western blot, immunohistochemistry and immunofluorescence.

The antiserum is diluted with an equal amount of PBS and absorbed with E. coli proteins.
recombinant murine SQSTM1 (A170)(AA254-333) containing T7 tag at the N-terminal end and His tag at the carboxy-terminal end
Specific for mouse and rat SQSTM1 (A170/ZIP).
Slightly reactive with human SQSTM1 (p62).
Working Dilution

  • Western blot 1 : 200

  • Immunohistochemistry 1 : 1,000

  • Immuno fluorescence 1 : 1,000


  1. Ishii, T., Yanagawa, T., Kawane, T., Yuki, K., Seita, J., Yoshida, H. and Bannai, S.: "Transcription factor Nrf2 coordinately regulates a group of oxidative stress-inducible genes in macrophages", Biochem. Biophys. Res. Commun., 226, 456-60 (1996).

  2. Ishii, T., Itoh, K., Takahashi, S., Sato, H., Yanagawa, T., Katoh, Y., Bannai, S. and Yamamoto, M.: "Transcription factor Nrf2 coordinately regulates a group of oxidative stress-inducible genes in macrophages", J. Biol. Chem., 275, 16023-9 (2000).

  3. Komatsu, M., Waguri, S., Koike, M., Sou, Y.S., Ueno, T., Hara, T., Mizushima, N., Iwata, J., Ezaki, J., Murata, S., Hamazaki, J., Nishito, Y., Iemura, S., Natsume, T., Yanagawa, T., Uwayama, J., Warabi, E., Yoshida, H., Ishii, T., Kobayashi, A., Yamamoto, M., Yue, Z., Uchiyama, Y., Kominami, E. and Tanaka, K.: "Homeostatic levels of p62 control cytoplasmic inclusion body formation in autophagy-deficient mice", Cell, 131, 1149-63 (2007).

  4. Nakaso, K., Kitayama, M., Ishii, T., Bannai, S., Yanagawa, T., Kimura, K., Nakashima, K., Ohama, E. and Yamada, K.: "Effects of kainate-mediated excitotoxicity on the expression of rat counterparts of A170 and MSP23 stress proteins in the brain", Brain Res. Mol. Brain Res., 69, 155-63 (1999).


Antibody Information

Antigen SQSTM1/A170/p62
Antigen Alias sequestosome 1
PDB3, ZIP3, p60, p62
sequestosome-1 (ubiquitin-binding protein p62, p60, EBIAP, PDB3, ZIP3)
sequestosome-1 (SQSTM1, ubiquitin-binding protein p62, p60, EBIAP, PDB3, ZIP3)

Manufacturer Information


  • Anti Sequestosome 1, Rabbit

For research use or further manufacturing use only. Not for use in diagnostic procedures.

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