Parkinson's Disease-related Antibody

Phosphorylated α-Syn Antibody

Phosphorylated α-Synuclein, Monoclonal Antibody
Cat. No. Product Name Grade Package Size
015-25191 Anti Phosphorylated α-Synuclein, Monoclonal Antibody (pSyn#64) For immunochemistry 50 µL
Antibody Information
Antigen α-Synuclein pS129 Application WB, IHC Isotype IgG ■Immunostaining image
Brain section of dementia with Lewy bodies
Immunostaining image of α-Synuclein Antibody
Courtesy of Iwatsubo,T., Graduate School of Medicine, The University of Tokyo
Antigen information Amino acid residue 124-134 of human α-synuclein with phosphorylated serine at position 129 Species cross reactivity Human, Mouse, Rat Label Unlabeled
Antigen synonyms SNCA, PARK1, PARK4, NACP, PD1 Host Mouse Clone No. pSyn#64
Summary Lewy body, a substance specifically found in nerve cells affected with Parkinson’s disease or dementia with Lewy body (DLB), contains α-synuclein protein of which serine at 129th position is specifically phosphorylated. This antibody does not react with normal α-synuclein, but recognizes only accumulated phosphorylated α-synuclein. This antibody is available for research in the Lewy body-related pathology.
Reference 1) Fujiwara. H., et al,.: Nature Cell Biology, 4, 160 (2002).
2) Saito. Y,.: Journal of Neuropathology and Experimental Neurology, 62, 644 (2003).
Biotin conjugated Phosphorylated α-Synuclein, Monoclonal Antibody
Cat. No. Product Name Grade Package Size
010-26481 Anti Phosphorylated α-Synuclein, Monoclonal Antibody (pSyn#64), Biotin-conjugated For immunochemistry 100 µL
Antibody Information
Antigen α-Synuclein pS129 Application IHC, ICC Isotype IgG ■Immunostaining image
Mouse brain section
Immunostaining image of α-Synuclein Antibody
Courtesy of Kuwabara and Iwatsubo, Graduate School of Medicine, The University of Tokyo
Antigen information Amino acid residue 124-134 of human α-synuclein with phosphorylated serine at position 129 Species cross reactivity Human, Mouse, Rat Label Biotin
Antigen synonyms SNCA, PARK1, PARK4, NACP, PD1 Host Mouse Clone No. pSyn#64
Summary This is an biotin-conjugated antibody of above Anti Phosphorylated α-Synuclein, Monoclonal Antibody (pSyn#64) (Wako Cat. No. 015-25191).

Parkin Antibody

Cat. No. Product Name Grade Package Size
010-27341 Anti Parkin, Monoclonal Antibody (Par6) For immunochemistry 10 µL
016-27343 50 µL
Antibody Information
Antigen Parkin Application WB Isotype IgG2·κ ■WB image
Western Blotting image of Parkin antibody
Lane1: Rat brain lysate
Lane2: Mouse brain lysate
Lane3: PC12 cell
Lane4: Hela cell
Lane5: HEK293T cell
Lane6: HEK293T cell
 express Human Parkin
Antigen information Full length recombinant human Parkin Species cross reactivity Human, Mouse, Rat Label Unlabeled
Antigen synonyms PRKN, Parkin RBRE4 ubiquitin protein ligase Host Mouse Clone No. Par6
Summary Parkin is an ubiquitin protein ligase about 50kDa that is a 465 amino acid with N-terminal ubiquitin-like domain. Parkin is a familial Parkinson disease (PD) gene product. PD is one of the most frequency neurodegenerative diseases. This item is a mouse monoclonal antibody which reacts with Parkin.
Reference

ASK1 Antibody

ASK1, Monoclonal Antibody
Cat. No. Product Name Grade Package Size
010-22341 Anti ASK1, Monoclonal Antibody (TC003) For immunochemistry 50 µg
Antibody Information
Antigen ASK1 Application WB Isotype IgG ■WB image
Bone marrow derived macrophage lysate of wild mouse (+/+) and ASK1 KO mouse(-/-)
Western Blotting image of ASK1 antibody
Courtesy of Maruyama,J. and Noguchi,T. , Graduate School of Pharmaceutical Sciences, The University of Tokyo
Antigen information Amino acid residue 948-1380 of Mouse ASK1 Species cross reactivity Human, Mouse Label Unlabeled
Antigen synonyms MAP3K5, MEKK5, MAPKKK5, Apoptosis Signal Regulating Kinase1 Host Mouse Clone No. TC003
Summary ASK1 is a most upstream protein kinase of MAP kinase cascade. It is activated by cell stress such as active oxygen and induce apoptosis and cell differentiation. Activation of ASK1 was reported to induce neuronal death in Alzheimer’s disease and ALS.
Reference Ichijo, H., Nishida, E., Irie, K., et al.: SCIENCE, 275, 90-94 (1997).
Phosphorylated ASK1, Monoclonal Antibody
Cat. No. Product Name Grade Package Size
017-22351 Anti Phosphorylated ASK1, Monoclonal Antibody (PA214) For immunochemistry 50 µg
Antibody Information
Antigen ASK1 pThr838 Application WB Isotype IgG ■WB image
TA Lane:ASK1 overexpression
 cells with modification of
 Thr838 to Ala
WT Lane:wild type human ASK1
 overexpression cells
Stim Lane:wild type human ASK1
 overexpression cells
 stimulated by H2O2

Western Blotting image of  Phosphorylated  ASK1 antibody
Courtesy of Maruyama,J. and Noguchi,T., Graduate School of Pharmaceutical Sciences, The University of Tokyo
Antigen information Amino acid residue 835-845 of human ASK1 with phosphorylated serine at position 838 Species cross reactivity Human, Mouse Label Unlabeled
Antigen synonyms MAP3K5, MEKK5, MAPKKK5, Apoptosis Signal Regulating Kinase1 Host Mouse Clone No. PA214
Summary ASK1 is a most upstream protein kinase of MAP kinase cascade. It is activated by cell stress such as active oxygen and induce apoptosis and cell differentiation. Activation of ASK1 was reported to induce neuronal death in Alzheimer’s disease and ALS.
Reference Ichijo, H., Nishida, E., Irie, K., et al.: SCIENCE, 275, 90-94 (1997).

Autophagy related Antibody

Autophagy related Antibody, Atg7
Cat. No. Product Name Grade Package Size
013-22831 Anti Human Atg7, Rabbit For immunochemistry 50 µL
Antibody Information
Antigen Atg7 Application WB Isotype IgG ■WB
Lysate of Mouse MEF
Western Blotting image of  Atg7 antibody
Courtesy of Ueno, T., Juntendo University School of Medicine
Antigen information KLH-conjugated Human Atg7 aa 556-571 Species cross reactivity Human, Rat, Mouse Label Unlabeled
Antigen synonyms APG7L, GSA7 Host Rabbit Clone No. Polyclonal
Summary Atg7 is an essential factor to assemble autophagosomes during the process of autophagy. It is an E1-like enzyme that binds to ubiquitin-like proteins Atg8 and Atg12. Dysfunction of Autophagy factors have been reported in neurodegenerative diseases such as Parkinson’s disease.
Reference
Autophagy related Antibody, LC3
Cat. No. Product Name Grade Package Size
010-22841 Anti Rat LC3, Rabbit For immunochemistry 50 µL
Antibody Information
Antigen LC3 Application WB, ICC Isotype IgG ■Immunostaining image
Hela cells
Immunostaining image of  LC3 antibody
Courtesy of Kikkawa, M., Juntendo University Graduate School of Medicine
Antigen information KLH conjugated LC3B aa 5-18 Species cross reactivity Human, Rat, Mouse Label Unlabeled
Antigen synonyms LC3B, MAP1LC3B, ATG8F, MAP1ALC3 Host Rabbit Clone No. Polyclonal
Summary LC3 is a mammalian analogue of Atg8 essential factor of autophagy in budding yeast. LC3 become LC3-I by removing C-terminal shortly after synthesis in cytoplasm. It is passed to E1-like enzyme (Atg7) and E2-like enzyme (Atg3) and it become LC3-II by binding with phospholipid. LC3-II bind to autophagosome membrane. This is why LC3 is used as a marker of autophagy. Dysfunction of autophagy factors have been reported in neurodegenerative diseases such as Parkinson’s disease. This antibody recognize both LC3-I and LC3-II.
Reference
Autophagy related Antibody, SQSTM1/A170/p62
Cat. No. Product Name Grade Package Size
018-22141 Anti SQSTM1/A170/p62, Rabbit For immunochemistry 100 µL
Antibody Information
Antigen SQSTM1, A170, p62 Application WB, IHC, IF Isotype IgG ■Immunostaining image
Rat basal ganglion
Immunostaining image of SQSTM1 antibody
Courtesy of Nakaso, K., Faculty of Medicine, Tottori University
Antigen information Mouse SQSTM1/A170/p62 PEST domain (T7 tag + amino acid 254-333 + His tag) recombinant Species cross reactivity Rat, Mouse Label Unlabeled
Antigen synonyms Sequestosome 1, OSIL, PDB3, ZIP3, EBIA, PORCA, p62B Host Rabbit Clone No. Polyclonal
Summary Sequestosome 1(SQSTM1)/A170(Mouse )/p62(human)/ZIP (Rat) is a ubiquitin binding protein and shows oxidative stress-dependent expression. Recently, it is reported that SQSTM1 binds to LC3, an autophagy related factor. It attracts attention because it may induce protein degradation from ubiquitin/proteosome system to autophagy system. In neurodegenerative diseases including Parkinson’s disease, dysfunction of autophagy factor is implicated.
Reference Ishii, T., et al.: J. Biol. Chem., 275, 16023 (2000).

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