Anti Laminin Antibodies
Laminin is a glycoprotein that constitutes the extracellular matrix (ECM) and plays an important role in cell adhesion, migration, and proliferation. In particular, laminin, together with type IV collagen, is a major component of the basement membrane. Fujifilm Wako offers guinea pig anti-laminin antibodies that are useful for multiplex staining.
What is Laminin?
Basic Structure of Laminin
Laminin is a glycoprotein with a molecular weight of approximately 900 kDa. It consists of three chains-α, β, and γ-that assemble into a cross-shaped structure. Each chain has multiple isoforms, and laminin types are named based on the combination of isoforms (for example, laminin composed of α1/β1/γ1 is referred to as laminin-111).
Laminin is well-known as a key component of the extracellular matrix (ECM) and plays an important role in cell adhesion, migration, and proliferation. In particular, laminin, together with type IV collagen, is considered a major component of the basement membrane. In the central nervous system, laminin is almost exclusively expressed in the basement membranes surrounding blood vessels and is used as a marker for these vessels. Previous studies have shown that the inner (endothelial) layer of the basement membrane in blood vessels contains laminin-411 and -511, while the outer (parenchymal) layer contains laminin-211 and -1111). Laminin also plays a critical role in the formation and function of the blood-brain barrier (BBB).
Anti Laminin, Guinea Pig
The “Anti Laminin , Guinea Pig” is a guinea pig polyclonal antibody, raised against Laminin2-6). It can be used to perform multiplex immunohistochemistry.
Antibody Information
| Clonality | Polyclonal |
|---|---|
| Antigen | Laminin isolated from Engelbreth-Holm-Swarm murine sarcoma basement membrane |
| Host | Guinea pig |
| Formulation | Antiserum diluted in PBS |
| Conjugate | Unconjugated |
| Cross-reactivity | Mouse, Rat |
| Application | Immunohistochemistry (Frozen Section) 1:500 * 6 hours of post-fixation is recommended |
Application Data
Immunohistochemistry
Species: Mouse
Site: Hippocampus
Sample: Frozen section
Antibody concentration: 1:500
Data by courtesy of
Dr. Miyata, Department of Applied Biology, Kyoto Institute of Technology
Species: Mouse
Site: Medulla oblongata (Area postrema)
Sample: Frozen section
Antibody concentration: 1:500
Data by courtesy of
Dr. Miyata, Department of Applied Biology, Kyoto Institute of Technology
References
- Halder, S. K. et al.: Neural Regen. Res., 18(12), 2557(2023).
The importance of laminin at the blood-brain barrier - Morita, S. et al.: Cell Tissue Res., 363, 497(2016).
Heterogeneous vascular permeability and alternative diffusion barrier in sensory circumventricular organs of adult mouse brain - Nishikawa, K. et al.: J. Neuroendocrinol., 29(2), (2017).
Structural Reconstruction of the Perivascular Space in the Adult Mouse Neurohypophysis During an Osmotic Stimulation - Takagi, S. et al.: J. Neuroimmunol., 331, 74(2019).
Microglia are continuously activated in the circumventricular organs of mouse brain - Muneoka, S. et al.: J. Neuroimmunol., 331, 58(2019).
TLR4 in circumventricular neural stem cells is a negative regulator for thermogenic pathways in the mouse brain - Murayama, S. et al.: J. Neuroimmunol., 334, 576973(2019).
Activation of microglia and macrophages in the circumventricular organs of the mouse brain during TLR2-induced fever and sickness responses
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Anti Laminin, Guinea Pig (Polyclonal Antibody)
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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