Anti Claudin-5 Antibodies
Claudin-5 is a tetra-transmembrane protein with approximately 23kDa, belongs to a member of Claudin family. It functions as a key component regulating tight junction of blood-brain barrier 1). These products are monoclonal antibodies which specifically recognize Claudin-5, and have neutralizing activity which reduces barrier function in blood-brain barrier model 1).They have no cross-reaction to Claudin-1-4,6,7 1).
1)Hashimoto, Y., et al.: J. Pharmacol. Exp. Ther., 363(2), 275(2017).
This product is sold under thelicense of Osaka University-Ehime University-FUJIFILM Wako Pure Chemical Corporation joint application patent.
PCT/JP2018/016975 [ANTI-CLDN-5 ANTIBODY, AND DRUG CONTAINING SAID ANTIBODY]
Antibody information
| Anti Claudin-5, Monoclonal Antibody (R9) | Anti Claudin-5, Monoclonal Antibody (M48) | |
|---|---|---|
| Code No. | 014-28101 | 011-28091 |
| Clone No. | R9 | M48 |
| Antigen | Vector expressing human claudin-5 full length accession#AAH19290 | Vector expressing human claudin-5 full length accession#AAH19290 |
| Buffer | PBS | PBS |
| Subclass | Rat IgG2b・κ | Mouse IgG3・κ |
| Cross-reactivity | Human, monkey | Human, monkey |
| Specificity | Claudin-5 No cross-reaction to Claudin-1-4,6,71) |
Claudin-5 No cross-reaction to Claudin-1-4,6,71) |
| Concentration | Indicated to the label First lot：1.1mg/mL |
Indicated to the label First lot:1.2mg/mL |
| Application | Flow cytometry 0.5-5μg/mL Neutralizing assay(in vitro) 90-150μg/mL |
Flow cytometry 0.5-5μg/mL Neutralizing assay(in vitro) 90-150μg/mL |
Application Data
Neutralizing assay in vitro BBB cell model
The barrier activity was evaluated by adding these antibodies (R9 and M48) were added to the blood-brain barrier cynomolgus cell model (BBB kit, PharmaCo-cell).
| (1)Trans-epithelial/endothelial electrical resistance (TEER) | (2) Substance permanent |
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| These antibodies(R9 and M48) reduced TEER. The effects were higher than C-CPEmt. |
These antibodies(R9 and M48) increased permanent of fluorescein isothiocyanate–labeled dextran(4kDa).The effects were higher than C-CPEmt. |
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For research use or further manufacturing use only. Not for use in diagnostic procedures.
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