Exosomes, from COLO201 cells, purified
In experiments involving extracellular vesicles (EVs), such as exosomes, using EVs confirmed to express marker proteins as positive controls allows verification of experimental success. Fujifilm Wako provides EVs isolated and purified from the cell culture supernatant of COLO201 cells using PS affinity method.
Product Overview
These products are extracellular vesicles (EVs) isolated and purified from the cell culture supernatant of COLO201 cells using the PS affinity method. They can be used as positive controls or calibrators in EV experiments.
Features
- High purity
- High stability
- Non-lyophilized
- CD9/CD63/CD81 positive
Specification
Properties: Solution
Formulation: EVs, TBS, EDTA, Polymer (stabilizer)
Protein concentration*: 10 μg/mL
* The protein concentration of each product was calculated from the signal values of the EV marker protein.
Exosomes, from COLO201 cells, purified(CD9) → CD9
Exosomes, from COLO201 cells, purified(CD63) → CD63
Exosomes, from COLO201 cells, purified(CD81) → CD81
Data
Particle Analysis by NTA
Using Nanoparticle Tracking Analysis (NTA) with NanoSight, particle size analysis was performed on “Exosomes, from COLO201 cells, purified(CD63)” and competitor’s products.
[Result]
Our product exhibited a more uniform particle size compared to competitors' product.
Structural observation by electron microscopy
The structure of “Exosomes, from COLO201 cells, purified(CD63)” was observed by electron microscopy after 15 cycles of freezing at -20 °C and thawing at room temperature.
Confirmation of EV marker protein expression by Western blotting
Expression of each EV marker protein
Non-reducing SDS-PAGE was performed for 10 ng/Lane of “Exosomes, from COLO201 cells, purified(CD63)”, and the EV marker proteins, CD9/CD63/CD81, were detected with antibodies.
Primary Antibody
Anti CD9, Monoclonal Antibody (1K) (Product No. 014-27763)
Anti CD63, Monoclonal Antibody (3-13) (Product No. 012-27063)
Anti CD81, Monoclonal Antibody (17B1) (Product No. 011-27773)
Secondary Antibody
HRP conjugated anti-mouse IgG
[Result]
Expression of each EV marker protein was confirmed.
Comparison with Other Competitors’ Products
“Exosomes, from COLO201 cells, purified(CD63)” and five EVs from other companies were compared to see whether CD63 signal could be detected. All were applied at a concentration of 10 ng/Lane based on the concentrations determined by each manufacturer.
Primary Antibody
Anti CD63, Monoclonal Antibody (3-13) (Product No. 012-27063)
Secondary Antibody
HRP conjugated anti-mouse IgG
[Result]
Only our product was able to detect CD63 signals under non-reducing conditions.
(1) Our Product
(2) EV from A549 cells
(3) EV from HEK293 cells
(4) EV from A549 cells
(5) EV from COLO1 cells
(6) EV from HCT116 cells
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Exosomes, from COLO201 cells, purified
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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