CultureSureR Y-27632
- for Cell Culture
- Specification Assay :
- 98.0+% (HPLC)
- Manufacturer :
- FUJIFILM Wako Pure Chemical Corporation
- Storage Condition :
- Keep at -20 degrees C.
- CAS RN® :
- 331752-47-7
- Molecular Formula :
- C14H21N3O・2HCl・H2O
- Molecular Weight :
- 338.27
- Structural Formula
- Label
- Packing
Comparison
|
Product Number
|
Package Size
|
Price
|
Inventory
|
|
---|---|---|---|---|---|
|
|
1mg
|
|
In stock in Japan |
|
|
|
5mg
|
|
In stock |
|
|
|
25mg
|
|
In stock in Japan |
|
|
|
100mg
|
|
In stock in Japan |
Document
Y-27632 Comparison Chart
ICH-Q7 | ISO9001 | Sterility test |
Viable count test |
Animal- derived- free |
Bacterial Endotoxin test |
Mycoplasma test |
Powder/ Liquid |
References* | |
---|---|---|---|---|---|---|---|---|---|
Y-27632 (GMP-compliant)** | ✓ | ✓ | - | ✓ | ✓ | ✓ | ✓ | Powder | - |
Y-27632, MF*** | - | ✓ | - | ✓ | ✓ | ✓ | ✓ | Powder | |
CultureSure® Y-27632 | - | ✓ | - | - | ✓ | ✓ | ✓ | Powder | |
CultureSure® 10mmol/L Y-27632 Solution, Animal-derived-free |
- | ✓ | ✓ | - | ✓ | ✓ | ✓ | Liquid Sterilized with filter |
* Click to move to the PubMed Central® search results.
** This product is complied with ICH-Q7 (GMP for APIs) as a raw material for commercial production of regenerative medicine products.
*** The manufacturing process and analytical procedure are validated.
Preparation
Solubility Data for Y-27632 dihydrochloride
C14H21N3O・2HCl・H2O = 338.27
Solvent | Conc. mg/mL | Conc. mmol/L | |
---|---|---|---|
Solubility | Water | 10mg/mL | 29.56 mmol/L |
Ethanol | 10mg/mL | 29.56 mmol/L |
Preparing Stock Solutions
C14H21N3O・2HCl・H2O = 338.27
Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
---|---|---|---|
1 mmol/L | 2.956 mL | 14.781 mL | 29.562 mL |
5 mmol/L | 0.591 mL | 2.956 mL | 5.912 mL |
10 mmol/L | 0.296 mL | 1.478 mL | 2.956 mL |
50 mmol/L | 0.059 mL | 0.296 mL | 0.591 mL |
References
- Watanabe, K., et al.: Nat. Biotechnol., 25, 681 (2007).
A ROCK inhibitor permits survival of dissociated human embryonic stem cells - Masashi, H., et al. : Genes Dev, 1, 36(1-2), 84–102 (2022).
Highly rigid H3.1/H3.2–H3K9me3 domains set a barrier for cell fate reprogramming in trophoblast stem cells - Yoshifumi, M., et al. : J Neurosci Res, 98(8), 1575–1587 (2020).).
Zonisamide promotes survival of human‐induced pluripotent stem cell‐derived dopaminergic neurons in the striatum of female rats - Kazutoshi, T., et al. : Cell Rep Methods, 2(2), 100155 (2022).
A stress-reduced passaging technique improves the viability of human pluripotent cells - Yu, K., et al. : eNeuro, 9(2), ENEURO.0442-21 (2022).
Characterization of Hypothalamic MCH Neuron Development in a 3D Differentiation System of Mouse Embryonic Stem Cells - Tomoya, U., et al. : Cell Rep Med, 2(6) 100298 (2021).
A muscle fatigue-like contractile decline was recapitulated using skeletal myotubes from Duchenne muscular dystrophy patient-derived iPSCs - Genki, N., et al. : eLife, 11, e77007 (2022).
Robotic search for optimal cell culture in regenerative medicine - Maobi, Z., et al. : Cell, 8(8), 933 (2019).
Estrogen and EGFR Pathways Regulate Notch Signaling in Opposing Directions for Multi-Ciliogenesis in the Fallopian Tube - Yu, K., et al. : Front Endocrinol (Lausanne), 13, 941166 (2022).
EpCAM Is a Surface Marker for Enriching Anterior Pituitary Cells From Human Hypothalamic-Pituitary Organoids - Sho, T., et al. : STAR Protoc, 3(2), 101360 (2022).
Purification of cardiomyocytes and neurons derived from human pluripotent stem cells by inhibition of de novo fatty acid synthesis - Takuro, T., et al. : Commun Biol, 5, 453, (2022).
Extremely low-frequency pulses of faint magnetic field induce mitophagy to rejuvenate mitochondria - Tomoki, Y., et al. : Mol Ther Methods Clin Dev, 10(22), 263–278 (2021).
Monolayer platform using human biopsy-derived duodenal organoids for pharmaceutical research - Bin, X., et al. : J Neurochem, 153(6), e14949 (2020).
Transplantation of iPS‐derived vascular endothelial cells improves white matter ischemic damage - Hiroyuki, N., et al. : Front Physiol, 10, 818 (2019).
Geometrical Patterning and Constituent Cell Heterogeneity Facilitate Electrical Conduction Disturbances in a Human Induced Pluripotent Stem Cell-Based Platform: An In vitro Disease Model of Atrial Arrhythmias - Yayoi, H., et al. : Front Pharmacol, 12, 680618 (2021).
High-Throughput Drug Screening System Based on Human Induced Pluripotent Stem Cell-Derived Atrial Myocytes ∼ A Novel Platform to Detect Cardiac Toxicity for Atrial Arrhythmias - Masahiko, A., et al. : Nat Commun, 12, 4507 (2021).
Therapeutic manipulation of IKBKAP mis-splicing with a small molecule to cure familial dysautonomia - Maobi, Z., et al. : Biomolecules, 10(9), 1270 (2020).
Fallopian Tube Basal Stem Cells Reproducing the Epithelial Sheets In Vitro—Stem Cell of Fallopian Epithelium - Junko, Y., et al. : iScience, 25(7), 104538 (2022).
StemPanTox: A fast and wide-target drug assessment system for tailor-made safety evaluations using personalized iPS cells - Keitaro, K., et al. : J Endocr Soc, 3(11), 1969–1978 (2019).
Pathogenesis of Anti–PIT-1 Antibody Syndrome: PIT-1 Presentation by HLA Class I on Anterior Pituitary Cells - Koichiro, O., et al. :Sci Rep, 8, 3615 (2018).
Vasopressin-secreting neurons derived from human embryonic stem cells through specific induction of dorsal hypothalamic progenitors - Kengo, K., et al. : Sci Rep, 11, 22476 (2021)
Requirement of direct contact between chondrocytes and macrophages for the maturation of regenerative cartilage - Hayato, F., et al. : J Endocr Soc, 3(11), 1969–1978 (2019).
Alpha‐synuclein dynamics in induced pluripotent stem cell‐derived dopaminergic neurons from a Parkinson’s disease patient (PARK4) with SNCA triplication - Yoji, K., et al. : Life Sci Alliance, 4(5), e202000974 (2021).
GATA transcription factors, SOX17 and TFAP2C, drive the human germ-cell specification program - Eri, N., et al. : Biochem Biophys Rep, 29, 101195 (2022).
Slow diffusion on the monolayer culture enhances auto/paracrine effects of Noggin in differentiation of human iPS cells induced by BMP - Kun, H., et al. : Sci Rep, 10, 2558 (2020).
Inhibition of cyclooxygenase-1 by nonsteroidal anti-inflammatory drugs demethylates MeR2 enhancer and promotes Mbnl1 transcription in myogenic cells - Yusuke, T., et al. : Int J Mol Sci, 20(6), 1331 (2019).
ROCK2 Regulates Monocyte Migration and Cell to Cell Adhesion in Vascular Endothelial Cells
Overview / Applications
Outline | Y-27632 is selective and potent ROCK inhibitor. Y-27632 plays various roles including contraction of vascular smooth muscle cells via ROCK signaling pathways. Furthermore, it suppresses the cell death of human embryonic stem cells (hESCs) and human induced Pluripotent Stem Cells (hiPSCs) at cell dispersion, and that it increases the survival rate of hESCs and hiPSCs during cryopreservation. |
---|---|
Precautions for Use | Packed on inactive gas |
Property
Appearance | White - pale yellow, crystalline powder - powder |
---|---|
Boiling Point | about 85 degrees C |
Specific Rotation | +2 - +10 degrees (D/20 degrees C)(c=0.5,CH3OH) |
Purity | Bacterial endotoxins : <0.25EU/mg |
Manufacturer Information
Alias
- (R)-(+)-trans-N-(4-pyridyl)-4-(1-aminoethyl)-cyclohexanecarboxamide Dihydrochloride Monohydrate
For research use or further manufacturing use only. Not for use in diagnostic procedures.
Product content may differ from the actual image due to minor specification changes etc.
If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.